Plants with genetically encoded autoluminescence

Autoluminescent plants that express a bacterial bioluminescence gene cluster(1) have not been widely adopted due to requisite expression in plastids and low light output. Alternatively, we have engineered tobacco lines expressing a fungal bioluminescent system(2), which converts caffeic acid present...

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Detalles Bibliográficos
Autores principales: Mitiouchkina, Tatiana, Mishin, Alexander S., Somermeyer, Louisa Gonzalez, Markina, Nadezhda M., Chepurnyh, Tatiana V., Guglya, Elena B., Karataeva, Tatiana A., Palkina, Kseniia A., Shakhova, Ekaterina S., Fakhranurova, Liliia I., Chekova, Sofia V., Tsarkova, Aleksandra S., Golubev, Yaroslav V., Negrebetsky, Vadim V., Dolgushin, Sergey A., Shalaev, Pavel V., Shlykov, Dmitry, Melnik, Olesya A., Shipunova, Victoria O., Deyev, Sergey M., Bubyrev, Andrey I., Pushin, Alexander S., Choob, Vladimir V., Dolgov, Sergey V., Kondrashov, Fyodor A., Yampolsky, Ilia V., Sarkisyan, Karen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610436/
https://www.ncbi.nlm.nih.gov/pubmed/32341562
http://dx.doi.org/10.1038/s41587-020-0500-9
Descripción
Sumario:Autoluminescent plants that express a bacterial bioluminescence gene cluster(1) have not been widely adopted due to requisite expression in plastids and low light output. Alternatively, we have engineered tobacco lines expressing a fungal bioluminescent system(2), which converts caffeic acid present in all plants into luciferin, and report self-sustained luminescence easily visible to the naked eye. Our findings might underpin development of a suite of imaging tools for plants.

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