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Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain

Open access post-mortem transcriptome atlases such as the Allen Human Brain Atlas (AHBA) can inform us about mRNA expression of numerous proteins of interest across the whole brain, while in vivo protein binding in the human brain can be quantified by means of neuroreceptor positron emission tomogra...

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Autores principales: Komorowski, Arkadiusz, Weidenauer, Ana, Murgaš, Matej, Sauerzopf, Ulrich, Wadsak, Wolfgang, Mitterhauser, Markus, Bauer, Martin, Hacker, Marcus, Praschak-Rieder, Nicole, Kasper, Siegfried, Lanzenberger, Rupert, Willeit, Matthäus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610745/
https://www.ncbi.nlm.nih.gov/pubmed/32818617
http://dx.doi.org/10.1016/j.neuroimage.2020.117270
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author Komorowski, Arkadiusz
Weidenauer, Ana
Murgaš, Matej
Sauerzopf, Ulrich
Wadsak, Wolfgang
Mitterhauser, Markus
Bauer, Martin
Hacker, Marcus
Praschak-Rieder, Nicole
Kasper, Siegfried
Lanzenberger, Rupert
Willeit, Matthäus
author_facet Komorowski, Arkadiusz
Weidenauer, Ana
Murgaš, Matej
Sauerzopf, Ulrich
Wadsak, Wolfgang
Mitterhauser, Markus
Bauer, Martin
Hacker, Marcus
Praschak-Rieder, Nicole
Kasper, Siegfried
Lanzenberger, Rupert
Willeit, Matthäus
author_sort Komorowski, Arkadiusz
collection PubMed
description Open access post-mortem transcriptome atlases such as the Allen Human Brain Atlas (AHBA) can inform us about mRNA expression of numerous proteins of interest across the whole brain, while in vivo protein binding in the human brain can be quantified by means of neuroreceptor positron emission tomography (PET). By combining both modalities, the association between regional gene expression and receptor distribution in the living brain can be approximated. Here, we compare the characteristics of D(2) and D(3) dopamine receptor distribution by applying the dopamine D(2/3) receptor agonist radioligand [(11)C]-(+)-PHNO and human gene expression data. Since [(11)C]-(+)-PHNO has a higher affinity for D(2) compared to D(2) receptors, we hypothesized that there is a stronger relationship between D(2/3) non-displaceable binding potentials (BP(ND)) and D(3) mRNA expression. To investigate the relationship between D(2/3) BP(ND) and mRNA expression of DRD2 and DRD3 we performed [(11)C]-(+)-PHNO PET scans in 27 healthy subjects (12 females) and extracted gene expression data from the AHBA. We also calculated D(2)/D(3) mRNA expression ratios to imitate the mixed D 2/3 signal of [(11)C]-(+)-PHNO. In accordance with our a priori hypothesis, a strong correlation between [(11)C]-(+)-PHNO and DRD3 expression was found. However, there was no significant correlation with DRD2 expression. Calculated D(2)/D(3) mRNA expression ratios also showed a positive correlation with [(11)C]-(+)-PHNO binding, reflecting the mixed D(2/3) signal of the radioligand. Our study supports the usefulness of combining gene expression data from open access brain atlases with in vivo imaging data in order to gain more detailed knowledge on neurotransmitter signaling.
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spelling pubmed-76107452021-12-01 Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain Komorowski, Arkadiusz Weidenauer, Ana Murgaš, Matej Sauerzopf, Ulrich Wadsak, Wolfgang Mitterhauser, Markus Bauer, Martin Hacker, Marcus Praschak-Rieder, Nicole Kasper, Siegfried Lanzenberger, Rupert Willeit, Matthäus Neuroimage Article Open access post-mortem transcriptome atlases such as the Allen Human Brain Atlas (AHBA) can inform us about mRNA expression of numerous proteins of interest across the whole brain, while in vivo protein binding in the human brain can be quantified by means of neuroreceptor positron emission tomography (PET). By combining both modalities, the association between regional gene expression and receptor distribution in the living brain can be approximated. Here, we compare the characteristics of D(2) and D(3) dopamine receptor distribution by applying the dopamine D(2/3) receptor agonist radioligand [(11)C]-(+)-PHNO and human gene expression data. Since [(11)C]-(+)-PHNO has a higher affinity for D(2) compared to D(2) receptors, we hypothesized that there is a stronger relationship between D(2/3) non-displaceable binding potentials (BP(ND)) and D(3) mRNA expression. To investigate the relationship between D(2/3) BP(ND) and mRNA expression of DRD2 and DRD3 we performed [(11)C]-(+)-PHNO PET scans in 27 healthy subjects (12 females) and extracted gene expression data from the AHBA. We also calculated D(2)/D(3) mRNA expression ratios to imitate the mixed D 2/3 signal of [(11)C]-(+)-PHNO. In accordance with our a priori hypothesis, a strong correlation between [(11)C]-(+)-PHNO and DRD3 expression was found. However, there was no significant correlation with DRD2 expression. Calculated D(2)/D(3) mRNA expression ratios also showed a positive correlation with [(11)C]-(+)-PHNO binding, reflecting the mixed D(2/3) signal of the radioligand. Our study supports the usefulness of combining gene expression data from open access brain atlases with in vivo imaging data in order to gain more detailed knowledge on neurotransmitter signaling. 2020-12-01 2020-08-17 /pmc/articles/PMC7610745/ /pubmed/32818617 http://dx.doi.org/10.1016/j.neuroimage.2020.117270 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Article
Komorowski, Arkadiusz
Weidenauer, Ana
Murgaš, Matej
Sauerzopf, Ulrich
Wadsak, Wolfgang
Mitterhauser, Markus
Bauer, Martin
Hacker, Marcus
Praschak-Rieder, Nicole
Kasper, Siegfried
Lanzenberger, Rupert
Willeit, Matthäus
Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title_full Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title_fullStr Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title_full_unstemmed Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title_short Association of dopamine D(2/3) receptor binding potential measured using PET and [(11)C]-(+)-PHNO with post-mortem DRD(2/3) gene expression in the human brain
title_sort association of dopamine d(2/3) receptor binding potential measured using pet and [(11)c]-(+)-phno with post-mortem drd(2/3) gene expression in the human brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610745/
https://www.ncbi.nlm.nih.gov/pubmed/32818617
http://dx.doi.org/10.1016/j.neuroimage.2020.117270
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