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Specific detection of form IA RubisCO genes in chemoautotrophic bacteria
The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin–Benson–Bassham cycle for CO(2) fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting c...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610800/ https://www.ncbi.nlm.nih.gov/pubmed/29797590 http://dx.doi.org/10.1002/jobm.201800136 |
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author | Allreider, Albin Bogensperger, Teresa |
author_facet | Allreider, Albin Bogensperger, Teresa |
author_sort | Allreider, Albin |
collection | PubMed |
description | The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin–Benson–Bassham cycle for CO(2) fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting chemoautotrophs are hampered in habitats dominated by phototrophs. Here, we report the development of a gene marker that specifically detects form IA RubisCO genes in bacteria, excluding photoautotrophic representatives. The high specificity of the PCR assay was confirmed by sequence analysis of DNA obtained from the photic zone of six lakes, were chemoautotrophs are outnumbered by Cyanobacteria also using form IA RubisCO for CO(2) assimilation. |
format | Online Article Text |
id | pubmed-7610800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-76108002021-05-18 Specific detection of form IA RubisCO genes in chemoautotrophic bacteria Allreider, Albin Bogensperger, Teresa J Basic Microbiol Article The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin–Benson–Bassham cycle for CO(2) fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting chemoautotrophs are hampered in habitats dominated by phototrophs. Here, we report the development of a gene marker that specifically detects form IA RubisCO genes in bacteria, excluding photoautotrophic representatives. The high specificity of the PCR assay was confirmed by sequence analysis of DNA obtained from the photic zone of six lakes, were chemoautotrophs are outnumbered by Cyanobacteria also using form IA RubisCO for CO(2) assimilation. 2018-08-01 2018-05-24 /pmc/articles/PMC7610800/ /pubmed/29797590 http://dx.doi.org/10.1002/jobm.201800136 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the Creative Commons Attribution (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Allreider, Albin Bogensperger, Teresa Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title | Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title_full | Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title_fullStr | Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title_full_unstemmed | Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title_short | Specific detection of form IA RubisCO genes in chemoautotrophic bacteria |
title_sort | specific detection of form ia rubisco genes in chemoautotrophic bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610800/ https://www.ncbi.nlm.nih.gov/pubmed/29797590 http://dx.doi.org/10.1002/jobm.201800136 |
work_keys_str_mv | AT allreideralbin specificdetectionofformiarubiscogenesinchemoautotrophicbacteria AT bogenspergerteresa specificdetectionofformiarubiscogenesinchemoautotrophicbacteria |