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Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity

Multidimensional single cell-analyses of T cells have fueled the debate about either extensive plasticity or “mixed” priming of T helper cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant o...

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Detalles Bibliográficos
Autores principales: Hiltensperger, Michael, Beltrán, Eduardo, Kant, Ravi, Tyystjärvi, Sofia, Lepennetier, Gildas, Moreno, Helena Domínguez, Bauer, Isabel J., Grassmann, Simon, Jarosch, Sebastian, Schober, Kilian, Buchholz, Veit R., Kenet, Selin, Gasperi, Christiane, Öllinger, Rupert, Rad, Roland, Muschaweckh, Andreas, Sie, Christopher, Aly, Lilian, Knier, Benjamin, Garg, Garima, Afzali, Ali M., Gerdes, Lisa Ann, Kümpfel, Tania, Franzenburg, Sören, Kawakami, Naoto, Hemmer, Bernhard, Busch, Dirk H., Misgeld, Thomas, Dornmair, Klaus, Korn, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7611097/
https://www.ncbi.nlm.nih.gov/pubmed/34099917
http://dx.doi.org/10.1038/s41590-021-00948-8
Descripción
Sumario:Multidimensional single cell-analyses of T cells have fueled the debate about either extensive plasticity or “mixed” priming of T helper cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model of multiple sclerosis are distinct. i-T cells were Cxcr6(+) and m-T cells expressed P2rx7. Notably, m-T cells infiltrated white matter while i-T cells were also recruited to grey matter. Therefore, we propose that the definition of T helper cell subsets by their site of priming might guide an advanced understanding of T helper cell biology in health and disease.