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Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity
Multidimensional single cell-analyses of T cells have fueled the debate about either extensive plasticity or “mixed” priming of T helper cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant o...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7611097/ https://www.ncbi.nlm.nih.gov/pubmed/34099917 http://dx.doi.org/10.1038/s41590-021-00948-8 |
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author | Hiltensperger, Michael Beltrán, Eduardo Kant, Ravi Tyystjärvi, Sofia Lepennetier, Gildas Moreno, Helena Domínguez Bauer, Isabel J. Grassmann, Simon Jarosch, Sebastian Schober, Kilian Buchholz, Veit R. Kenet, Selin Gasperi, Christiane Öllinger, Rupert Rad, Roland Muschaweckh, Andreas Sie, Christopher Aly, Lilian Knier, Benjamin Garg, Garima Afzali, Ali M. Gerdes, Lisa Ann Kümpfel, Tania Franzenburg, Sören Kawakami, Naoto Hemmer, Bernhard Busch, Dirk H. Misgeld, Thomas Dornmair, Klaus Korn, Thomas |
author_facet | Hiltensperger, Michael Beltrán, Eduardo Kant, Ravi Tyystjärvi, Sofia Lepennetier, Gildas Moreno, Helena Domínguez Bauer, Isabel J. Grassmann, Simon Jarosch, Sebastian Schober, Kilian Buchholz, Veit R. Kenet, Selin Gasperi, Christiane Öllinger, Rupert Rad, Roland Muschaweckh, Andreas Sie, Christopher Aly, Lilian Knier, Benjamin Garg, Garima Afzali, Ali M. Gerdes, Lisa Ann Kümpfel, Tania Franzenburg, Sören Kawakami, Naoto Hemmer, Bernhard Busch, Dirk H. Misgeld, Thomas Dornmair, Klaus Korn, Thomas |
author_sort | Hiltensperger, Michael |
collection | PubMed |
description | Multidimensional single cell-analyses of T cells have fueled the debate about either extensive plasticity or “mixed” priming of T helper cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model of multiple sclerosis are distinct. i-T cells were Cxcr6(+) and m-T cells expressed P2rx7. Notably, m-T cells infiltrated white matter while i-T cells were also recruited to grey matter. Therefore, we propose that the definition of T helper cell subsets by their site of priming might guide an advanced understanding of T helper cell biology in health and disease. |
format | Online Article Text |
id | pubmed-7611097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-76110972021-12-07 Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity Hiltensperger, Michael Beltrán, Eduardo Kant, Ravi Tyystjärvi, Sofia Lepennetier, Gildas Moreno, Helena Domínguez Bauer, Isabel J. Grassmann, Simon Jarosch, Sebastian Schober, Kilian Buchholz, Veit R. Kenet, Selin Gasperi, Christiane Öllinger, Rupert Rad, Roland Muschaweckh, Andreas Sie, Christopher Aly, Lilian Knier, Benjamin Garg, Garima Afzali, Ali M. Gerdes, Lisa Ann Kümpfel, Tania Franzenburg, Sören Kawakami, Naoto Hemmer, Bernhard Busch, Dirk H. Misgeld, Thomas Dornmair, Klaus Korn, Thomas Nat Immunol Article Multidimensional single cell-analyses of T cells have fueled the debate about either extensive plasticity or “mixed” priming of T helper cell subsets in vivo. Here, we developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model of multiple sclerosis are distinct. i-T cells were Cxcr6(+) and m-T cells expressed P2rx7. Notably, m-T cells infiltrated white matter while i-T cells were also recruited to grey matter. Therefore, we propose that the definition of T helper cell subsets by their site of priming might guide an advanced understanding of T helper cell biology in health and disease. 2021-07-01 2021-06-07 /pmc/articles/PMC7611097/ /pubmed/34099917 http://dx.doi.org/10.1038/s41590-021-00948-8 Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Hiltensperger, Michael Beltrán, Eduardo Kant, Ravi Tyystjärvi, Sofia Lepennetier, Gildas Moreno, Helena Domínguez Bauer, Isabel J. Grassmann, Simon Jarosch, Sebastian Schober, Kilian Buchholz, Veit R. Kenet, Selin Gasperi, Christiane Öllinger, Rupert Rad, Roland Muschaweckh, Andreas Sie, Christopher Aly, Lilian Knier, Benjamin Garg, Garima Afzali, Ali M. Gerdes, Lisa Ann Kümpfel, Tania Franzenburg, Sören Kawakami, Naoto Hemmer, Bernhard Busch, Dirk H. Misgeld, Thomas Dornmair, Klaus Korn, Thomas Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title | Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title_full | Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title_fullStr | Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title_full_unstemmed | Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title_short | Skin and gut imprinted T helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
title_sort | skin and gut imprinted t helper cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7611097/ https://www.ncbi.nlm.nih.gov/pubmed/34099917 http://dx.doi.org/10.1038/s41590-021-00948-8 |
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