Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome

Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various...

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Autores principales: Leung, Adelaine Kwun-Wai, Kondo, Yasushi, Krummel, Daniel A. Pomeranz, Li, Jade, Price, Stephen R., van Roon, Anne-Marie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7612351/
https://www.ncbi.nlm.nih.gov/pubmed/35154816
http://dx.doi.org/10.3390/cryst11080948
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author Leung, Adelaine Kwun-Wai
Kondo, Yasushi
Krummel, Daniel A. Pomeranz
Li, Jade
Price, Stephen R.
van Roon, Anne-Marie M.
author_facet Leung, Adelaine Kwun-Wai
Kondo, Yasushi
Krummel, Daniel A. Pomeranz
Li, Jade
Price, Stephen R.
van Roon, Anne-Marie M.
author_sort Leung, Adelaine Kwun-Wai
collection PubMed
description Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper.
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spelling pubmed-76123512022-02-10 Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome Leung, Adelaine Kwun-Wai Kondo, Yasushi Krummel, Daniel A. Pomeranz Li, Jade Price, Stephen R. van Roon, Anne-Marie M. Crystals (Basel) Article Cryo-electron microscopy has greatly advanced our understanding of how the spliceosome cycles through different conformational states to conduct the chemical reactions that remove introns from pre-mRNA transcripts. The Cryo-EM structures were built upon decades of crystallographic studies of various spliceosomal RNA-protein complexes. In this review we give an overview of the crystal structures solved in the Nagai group, utilizing many of the strategies to design crystal packing as described in the accompanying paper. 2021-08-15 /pmc/articles/PMC7612351/ /pubmed/35154816 http://dx.doi.org/10.3390/cryst11080948 Text en https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Leung, Adelaine Kwun-Wai
Kondo, Yasushi
Krummel, Daniel A. Pomeranz
Li, Jade
Price, Stephen R.
van Roon, Anne-Marie M.
Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title_full Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title_fullStr Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title_full_unstemmed Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title_short Engineering Crystal Packing in RNA-Protein Complexes II: A Historical Perspective from the Structural Studies of the Spliceosome
title_sort engineering crystal packing in rna-protein complexes ii: a historical perspective from the structural studies of the spliceosome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7612351/
https://www.ncbi.nlm.nih.gov/pubmed/35154816
http://dx.doi.org/10.3390/cryst11080948
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