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Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID

The epidermis of Caenorhabditis elegans is an essential tissue for survival as it contributes to the formation of the cuticle barrier, as well as facilitates developmental progression and animal growth. Most of the epidermis consists of the hyp7 hypodermal syncytium, the nuclei of which are largely...

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Autores principales: Katsanos, Dimitris, Ferrando-Marco, Mar, Razzaq, Iqrah, Aughey, Gabriel, Southall, Tony, Barkoulas, Michalis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613258/
https://www.ncbi.nlm.nih.gov/pubmed/34397094
http://dx.doi.org/10.1242/dev.199452
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author Katsanos, Dimitris
Ferrando-Marco, Mar
Razzaq, Iqrah
Aughey, Gabriel
Southall, Tony
Barkoulas, Michalis
author_facet Katsanos, Dimitris
Ferrando-Marco, Mar
Razzaq, Iqrah
Aughey, Gabriel
Southall, Tony
Barkoulas, Michalis
author_sort Katsanos, Dimitris
collection PubMed
description The epidermis of Caenorhabditis elegans is an essential tissue for survival as it contributes to the formation of the cuticle barrier, as well as facilitates developmental progression and animal growth. Most of the epidermis consists of the hyp7 hypodermal syncytium, the nuclei of which are largely generated by the seam cells that exhibit stem cell-like behaviour during development. How the seam cell progenitors differ transcriptionally from the differentiated hypodermis is poorly understood. Here, we introduce Targeted DamID (TaDa) in C. elegans as a method for identifying genes expressed within a tissue of interest without cell isolation. We show that TaDa signal enrichment profiles can be used to identify genes transcribed in the epidermis and use this method to resolve differences in gene expression between the seam cells and the hypodermis. We finally predict and functionally validate new transcription and chromatin factors acting in seam cell development. These findings provide insights into cell-type-specific gene expression profiles likely associated with epidermal cell fate patterning.
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spelling pubmed-76132582022-08-09 Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID Katsanos, Dimitris Ferrando-Marco, Mar Razzaq, Iqrah Aughey, Gabriel Southall, Tony Barkoulas, Michalis Development Article The epidermis of Caenorhabditis elegans is an essential tissue for survival as it contributes to the formation of the cuticle barrier, as well as facilitates developmental progression and animal growth. Most of the epidermis consists of the hyp7 hypodermal syncytium, the nuclei of which are largely generated by the seam cells that exhibit stem cell-like behaviour during development. How the seam cell progenitors differ transcriptionally from the differentiated hypodermis is poorly understood. Here, we introduce Targeted DamID (TaDa) in C. elegans as a method for identifying genes expressed within a tissue of interest without cell isolation. We show that TaDa signal enrichment profiles can be used to identify genes transcribed in the epidermis and use this method to resolve differences in gene expression between the seam cells and the hypodermis. We finally predict and functionally validate new transcription and chromatin factors acting in seam cell development. These findings provide insights into cell-type-specific gene expression profiles likely associated with epidermal cell fate patterning. 2021-09-03 /pmc/articles/PMC7613258/ /pubmed/34397094 http://dx.doi.org/10.1242/dev.199452 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
spellingShingle Article
Katsanos, Dimitris
Ferrando-Marco, Mar
Razzaq, Iqrah
Aughey, Gabriel
Southall, Tony
Barkoulas, Michalis
Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title_full Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title_fullStr Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title_full_unstemmed Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title_short Gene expression profiling of epidermal cell types in C. elegans using Targeted-DamID
title_sort gene expression profiling of epidermal cell types in c. elegans using targeted-damid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613258/
https://www.ncbi.nlm.nih.gov/pubmed/34397094
http://dx.doi.org/10.1242/dev.199452
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