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Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues

BACKGROUND: Testicular Adrenal Rest Tumors (TART) are a common complication of unknown cellular origin in patients with Congenital Adrenal Hyperplasia (CAH). These benign tumors have both adrenal and testicular characteristics and are hypothesized to either derive from cells of adrenal origin from t...

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Autores principales: Schröder, Mariska A.M., Sweep, Fred C.G.J., van Herwaarden, Antonius E., Mitchell, Rod T., Eliveld, Jitske, van Pelt, Ans M. M., Rowan, Alan E., Korbie, Darren, Stikkelbroeck, Nike M.M.L., Claahsen - van der Grinten, Hedi L., Span, Paul N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613903/
https://www.ncbi.nlm.nih.gov/pubmed/36047744
http://dx.doi.org/10.1530/EJE-22-0143
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author Schröder, Mariska A.M.
Sweep, Fred C.G.J.
van Herwaarden, Antonius E.
Mitchell, Rod T.
Eliveld, Jitske
van Pelt, Ans M. M.
Rowan, Alan E.
Korbie, Darren
Stikkelbroeck, Nike M.M.L.
Claahsen - van der Grinten, Hedi L.
Span, Paul N.
author_facet Schröder, Mariska A.M.
Sweep, Fred C.G.J.
van Herwaarden, Antonius E.
Mitchell, Rod T.
Eliveld, Jitske
van Pelt, Ans M. M.
Rowan, Alan E.
Korbie, Darren
Stikkelbroeck, Nike M.M.L.
Claahsen - van der Grinten, Hedi L.
Span, Paul N.
author_sort Schröder, Mariska A.M.
collection PubMed
description BACKGROUND: Testicular Adrenal Rest Tumors (TART) are a common complication of unknown cellular origin in patients with Congenital Adrenal Hyperplasia (CAH). These benign tumors have both adrenal and testicular characteristics and are hypothesized to either derive from cells of adrenal origin from the fetal adrenogonadal primordium or by atypical differentiation of adult Leydig-progenitor cells. OBJECTIVE: This study aims to unravel the identity and etiology of TART. METHODS: Co-expression of adrenal-specific CYP11B1 and Leydig cell-specific HSD17B3 in TART was studied using immunohistochemistry. We studied the possibility of TART being derived from atypical differentiation of adult Leydig-progenitor cells by the quantification of adrenal-specific enzyme expression upon ACTH-like stimulation of ex vivo cultured PDGFRA-positive cells. By comparing the transcriptome of TART (n=16) with the transcriptome of fetal adrenal (n=13), fetal testis (n=5), adult adrenal (n=11) and adult testis (n=10) tissues, we explored the identity of TART. RESULTS: We demonstrate co-expression of adrenal-specific CYP11B1 and testis-specific HSD17B3 in TART cells, indicating the existence of a distinct TART cell exhibiting both adrenal and testicular characteristics. Ex vivo cultured adult Leydig-progenitor cells did not express the ACTH-receptor MC2R but did express CYP11B1 upon stimulation. Unsupervised clustering of transcriptome data showed that TART was most similar to adult adrenal tissue, followed by adult testis tissue, and least similar to either fetal tissue. CONCLUSION: Our data suggest that TART is induced -most likely via activation of a cAMP/PKA dependent receptor-from a progenitor cell into a unique mature adrenal-like cell type, sometimes exhibiting both adrenal and testicular features.
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spelling pubmed-76139032022-12-09 Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues Schröder, Mariska A.M. Sweep, Fred C.G.J. van Herwaarden, Antonius E. Mitchell, Rod T. Eliveld, Jitske van Pelt, Ans M. M. Rowan, Alan E. Korbie, Darren Stikkelbroeck, Nike M.M.L. Claahsen - van der Grinten, Hedi L. Span, Paul N. Eur J Endocrinol Article BACKGROUND: Testicular Adrenal Rest Tumors (TART) are a common complication of unknown cellular origin in patients with Congenital Adrenal Hyperplasia (CAH). These benign tumors have both adrenal and testicular characteristics and are hypothesized to either derive from cells of adrenal origin from the fetal adrenogonadal primordium or by atypical differentiation of adult Leydig-progenitor cells. OBJECTIVE: This study aims to unravel the identity and etiology of TART. METHODS: Co-expression of adrenal-specific CYP11B1 and Leydig cell-specific HSD17B3 in TART was studied using immunohistochemistry. We studied the possibility of TART being derived from atypical differentiation of adult Leydig-progenitor cells by the quantification of adrenal-specific enzyme expression upon ACTH-like stimulation of ex vivo cultured PDGFRA-positive cells. By comparing the transcriptome of TART (n=16) with the transcriptome of fetal adrenal (n=13), fetal testis (n=5), adult adrenal (n=11) and adult testis (n=10) tissues, we explored the identity of TART. RESULTS: We demonstrate co-expression of adrenal-specific CYP11B1 and testis-specific HSD17B3 in TART cells, indicating the existence of a distinct TART cell exhibiting both adrenal and testicular characteristics. Ex vivo cultured adult Leydig-progenitor cells did not express the ACTH-receptor MC2R but did express CYP11B1 upon stimulation. Unsupervised clustering of transcriptome data showed that TART was most similar to adult adrenal tissue, followed by adult testis tissue, and least similar to either fetal tissue. CONCLUSION: Our data suggest that TART is induced -most likely via activation of a cAMP/PKA dependent receptor-from a progenitor cell into a unique mature adrenal-like cell type, sometimes exhibiting both adrenal and testicular features. 2022-11-01 2022-09-29 /pmc/articles/PMC7613903/ /pubmed/36047744 http://dx.doi.org/10.1530/EJE-22-0143 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
spellingShingle Article
Schröder, Mariska A.M.
Sweep, Fred C.G.J.
van Herwaarden, Antonius E.
Mitchell, Rod T.
Eliveld, Jitske
van Pelt, Ans M. M.
Rowan, Alan E.
Korbie, Darren
Stikkelbroeck, Nike M.M.L.
Claahsen - van der Grinten, Hedi L.
Span, Paul N.
Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title_full Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title_fullStr Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title_full_unstemmed Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title_short Transcriptional comparison of Testicular Adrenal Rest Tumors with fetal and adult tissues
title_sort transcriptional comparison of testicular adrenal rest tumors with fetal and adult tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613903/
https://www.ncbi.nlm.nih.gov/pubmed/36047744
http://dx.doi.org/10.1530/EJE-22-0143
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