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Intracellular chloride regulation mediates local sleep pressure in the cortex

Extended wakefulness is associated with reduced performance and the build-up of sleep pressure. In the cortex, this manifests as changes in network activity. These changes show local variation depending on the waking experience, and their underlying mechanisms represent targets for overcoming the ef...

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Autores principales: Alfonsa, Hannah, Burman, Richard J., Brodersen, Paul J. N., Newey, Sarah E., Mahfooz, Kashif, Yamagata, Tomoko, Panayi, Marios C., Bannerman, David M., Vyazovskiy, Vladyslav V., Akerman, Colin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614036/
https://www.ncbi.nlm.nih.gov/pubmed/36510112
http://dx.doi.org/10.1038/s41593-022-01214-2
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author Alfonsa, Hannah
Burman, Richard J.
Brodersen, Paul J. N.
Newey, Sarah E.
Mahfooz, Kashif
Yamagata, Tomoko
Panayi, Marios C.
Bannerman, David M.
Vyazovskiy, Vladyslav V.
Akerman, Colin J.
author_facet Alfonsa, Hannah
Burman, Richard J.
Brodersen, Paul J. N.
Newey, Sarah E.
Mahfooz, Kashif
Yamagata, Tomoko
Panayi, Marios C.
Bannerman, David M.
Vyazovskiy, Vladyslav V.
Akerman, Colin J.
author_sort Alfonsa, Hannah
collection PubMed
description Extended wakefulness is associated with reduced performance and the build-up of sleep pressure. In the cortex, this manifests as changes in network activity. These changes show local variation depending on the waking experience, and their underlying mechanisms represent targets for overcoming the effects of tiredness. Here we reveal a central role for intracellular chloride regulation, which sets the strength of postsynaptic inhibition via GABA(A) receptors in cortical pyramidal neurons. Wakefulness results in depolarizing shifts in the equilibrium potential for GABA(A) receptors, reflecting local activity-dependent processes during waking, and involving changes in chloride cotransporter activity. These changes underlie electrophysiological and behavioral markers of local sleep pressure within cortex — including the levels of slow-wave activity during non-rapid eye movement sleep — as well as low-frequency oscillatory activity and reduced performance levels in the sleep-deprived awake state. These findings identify chloride regulation as a crucial link between sleep–wake history, cortical activity and behavior.
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spelling pubmed-76140362023-06-12 Intracellular chloride regulation mediates local sleep pressure in the cortex Alfonsa, Hannah Burman, Richard J. Brodersen, Paul J. N. Newey, Sarah E. Mahfooz, Kashif Yamagata, Tomoko Panayi, Marios C. Bannerman, David M. Vyazovskiy, Vladyslav V. Akerman, Colin J. Nat Neurosci Article Extended wakefulness is associated with reduced performance and the build-up of sleep pressure. In the cortex, this manifests as changes in network activity. These changes show local variation depending on the waking experience, and their underlying mechanisms represent targets for overcoming the effects of tiredness. Here we reveal a central role for intracellular chloride regulation, which sets the strength of postsynaptic inhibition via GABA(A) receptors in cortical pyramidal neurons. Wakefulness results in depolarizing shifts in the equilibrium potential for GABA(A) receptors, reflecting local activity-dependent processes during waking, and involving changes in chloride cotransporter activity. These changes underlie electrophysiological and behavioral markers of local sleep pressure within cortex — including the levels of slow-wave activity during non-rapid eye movement sleep — as well as low-frequency oscillatory activity and reduced performance levels in the sleep-deprived awake state. These findings identify chloride regulation as a crucial link between sleep–wake history, cortical activity and behavior. 2023-01 2022-12-12 /pmc/articles/PMC7614036/ /pubmed/36510112 http://dx.doi.org/10.1038/s41593-022-01214-2 Text en https://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
spellingShingle Article
Alfonsa, Hannah
Burman, Richard J.
Brodersen, Paul J. N.
Newey, Sarah E.
Mahfooz, Kashif
Yamagata, Tomoko
Panayi, Marios C.
Bannerman, David M.
Vyazovskiy, Vladyslav V.
Akerman, Colin J.
Intracellular chloride regulation mediates local sleep pressure in the cortex
title Intracellular chloride regulation mediates local sleep pressure in the cortex
title_full Intracellular chloride regulation mediates local sleep pressure in the cortex
title_fullStr Intracellular chloride regulation mediates local sleep pressure in the cortex
title_full_unstemmed Intracellular chloride regulation mediates local sleep pressure in the cortex
title_short Intracellular chloride regulation mediates local sleep pressure in the cortex
title_sort intracellular chloride regulation mediates local sleep pressure in the cortex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614036/
https://www.ncbi.nlm.nih.gov/pubmed/36510112
http://dx.doi.org/10.1038/s41593-022-01214-2
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