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Advances in methods to analyse cardiolipin and their clinical applications

Cardiolipin (CL) is a mitochondria-exclusive phospholipid, primarily localised within the inner mitochondrial membrane, that plays an essential role in mitochondrial architecture and function. Aberrant CL content, structure, and localisation have all been linked to impaired mitochondrial activity an...

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Autores principales: Bautista, Javier S., Falabella, Micol, Flannery, Padraig J., Hanna, Michael G., Heales, Simon J.R., Pope, Simon A.S., Pitceathly, Robert D.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614147/
https://www.ncbi.nlm.nih.gov/pubmed/36751553
http://dx.doi.org/10.1016/j.trac.2022.116808
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author Bautista, Javier S.
Falabella, Micol
Flannery, Padraig J.
Hanna, Michael G.
Heales, Simon J.R.
Pope, Simon A.S.
Pitceathly, Robert D.S.
author_facet Bautista, Javier S.
Falabella, Micol
Flannery, Padraig J.
Hanna, Michael G.
Heales, Simon J.R.
Pope, Simon A.S.
Pitceathly, Robert D.S.
author_sort Bautista, Javier S.
collection PubMed
description Cardiolipin (CL) is a mitochondria-exclusive phospholipid, primarily localised within the inner mitochondrial membrane, that plays an essential role in mitochondrial architecture and function. Aberrant CL content, structure, and localisation have all been linked to impaired mitochondrial activity and are observed in the pathophysiology of cancer and neurological, cardiovascular, and metabolic disorders. The detection, quantification, and localisation of CL species is a valuable tool to investigate mitochondrial dysfunction and the pathophysiological mechanisms underpinning several human disorders. CL is measured using liquid chromatography, usually combined with mass spectrometry, mass spectrometry imaging, shotgun lipidomics, ion mobility spectrometry, fluorometry, and radiolabelling. This review summarises available methods to analyse CL, with a particular focus on modern mass spectrometry, and evaluates their advantages and limitations. We provide guidance aimed at selecting the most appropriate technique, or combination of techniques, when analysing CL in different model systems, and highlight the clinical contexts in which measuring CL is relevant.
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spelling pubmed-76141472023-02-06 Advances in methods to analyse cardiolipin and their clinical applications Bautista, Javier S. Falabella, Micol Flannery, Padraig J. Hanna, Michael G. Heales, Simon J.R. Pope, Simon A.S. Pitceathly, Robert D.S. Trends Analyt Chem Article Cardiolipin (CL) is a mitochondria-exclusive phospholipid, primarily localised within the inner mitochondrial membrane, that plays an essential role in mitochondrial architecture and function. Aberrant CL content, structure, and localisation have all been linked to impaired mitochondrial activity and are observed in the pathophysiology of cancer and neurological, cardiovascular, and metabolic disorders. The detection, quantification, and localisation of CL species is a valuable tool to investigate mitochondrial dysfunction and the pathophysiological mechanisms underpinning several human disorders. CL is measured using liquid chromatography, usually combined with mass spectrometry, mass spectrometry imaging, shotgun lipidomics, ion mobility spectrometry, fluorometry, and radiolabelling. This review summarises available methods to analyse CL, with a particular focus on modern mass spectrometry, and evaluates their advantages and limitations. We provide guidance aimed at selecting the most appropriate technique, or combination of techniques, when analysing CL in different model systems, and highlight the clinical contexts in which measuring CL is relevant. 2022-12 /pmc/articles/PMC7614147/ /pubmed/36751553 http://dx.doi.org/10.1016/j.trac.2022.116808 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
spellingShingle Article
Bautista, Javier S.
Falabella, Micol
Flannery, Padraig J.
Hanna, Michael G.
Heales, Simon J.R.
Pope, Simon A.S.
Pitceathly, Robert D.S.
Advances in methods to analyse cardiolipin and their clinical applications
title Advances in methods to analyse cardiolipin and their clinical applications
title_full Advances in methods to analyse cardiolipin and their clinical applications
title_fullStr Advances in methods to analyse cardiolipin and their clinical applications
title_full_unstemmed Advances in methods to analyse cardiolipin and their clinical applications
title_short Advances in methods to analyse cardiolipin and their clinical applications
title_sort advances in methods to analyse cardiolipin and their clinical applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614147/
https://www.ncbi.nlm.nih.gov/pubmed/36751553
http://dx.doi.org/10.1016/j.trac.2022.116808
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