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Increasing Complexity of the N-Glycome During Caenorhabditis Development
Caenorhabditis elegans is a frequently employed genetic model organism and has been the object of a wide range of developmental, genetic, proteomic, and glycomic studies. Here, using an off-line MALDI-TOF-MS approach, we have analyzed the N-glycans of mixed embryos and liquid- or plate-grown L4 larv...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Biochemistry and Molecular Biology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614267/ https://www.ncbi.nlm.nih.gov/pubmed/36717059 http://dx.doi.org/10.1016/j.mcpro.2023.100505 |
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author | Wilson, Iain B.H. Yan, Shi Jin, Chunsheng Dutkiewicz, Zuzanna Rendić, Dubravko Palmberger, Dieter Schnabel, Ralf Paschinger, Katharina |
author_facet | Wilson, Iain B.H. Yan, Shi Jin, Chunsheng Dutkiewicz, Zuzanna Rendić, Dubravko Palmberger, Dieter Schnabel, Ralf Paschinger, Katharina |
author_sort | Wilson, Iain B.H. |
collection | PubMed |
description | Caenorhabditis elegans is a frequently employed genetic model organism and has been the object of a wide range of developmental, genetic, proteomic, and glycomic studies. Here, using an off-line MALDI-TOF-MS approach, we have analyzed the N-glycans of mixed embryos and liquid- or plate-grown L4 larvae. Of the over 200 different annotatable N-glycan structures, variations between the stages as well as the mode of cultivation were observed. While the embryonal N-glycome appears less complicated overall, the liquid- and plate-grown larvae differ especially in terms of methylation of bisecting fucose, α-galactosylation of mannose, and di-β-galactosylation of core α1,6-fucose. Furthermore, we analyzed the O-glycans by LC–electrospray ionization–MS following β-elimination; especially the embryonal O-glycomes included a set of phosphorylcholine-modified structures, previously not shown to exist in nematodes. However, the set of glycan structures cannot be clearly correlated with levels of glycosyltransferase transcripts in developmental RNA-Seq datasets, but there is an indication for coordinated expression of clusters of potential glycosylation-relevant genes. Thus, there are still questions to be answered in terms of how and why a simple nematode synthesizes such a diverse glycome. |
format | Online Article Text |
id | pubmed-7614267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-76142672023-03-02 Increasing Complexity of the N-Glycome During Caenorhabditis Development Wilson, Iain B.H. Yan, Shi Jin, Chunsheng Dutkiewicz, Zuzanna Rendić, Dubravko Palmberger, Dieter Schnabel, Ralf Paschinger, Katharina Mol Cell Proteomics Research Caenorhabditis elegans is a frequently employed genetic model organism and has been the object of a wide range of developmental, genetic, proteomic, and glycomic studies. Here, using an off-line MALDI-TOF-MS approach, we have analyzed the N-glycans of mixed embryos and liquid- or plate-grown L4 larvae. Of the over 200 different annotatable N-glycan structures, variations between the stages as well as the mode of cultivation were observed. While the embryonal N-glycome appears less complicated overall, the liquid- and plate-grown larvae differ especially in terms of methylation of bisecting fucose, α-galactosylation of mannose, and di-β-galactosylation of core α1,6-fucose. Furthermore, we analyzed the O-glycans by LC–electrospray ionization–MS following β-elimination; especially the embryonal O-glycomes included a set of phosphorylcholine-modified structures, previously not shown to exist in nematodes. However, the set of glycan structures cannot be clearly correlated with levels of glycosyltransferase transcripts in developmental RNA-Seq datasets, but there is an indication for coordinated expression of clusters of potential glycosylation-relevant genes. Thus, there are still questions to be answered in terms of how and why a simple nematode synthesizes such a diverse glycome. American Society for Biochemistry and Molecular Biology 2023-01-28 /pmc/articles/PMC7614267/ /pubmed/36717059 http://dx.doi.org/10.1016/j.mcpro.2023.100505 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Wilson, Iain B.H. Yan, Shi Jin, Chunsheng Dutkiewicz, Zuzanna Rendić, Dubravko Palmberger, Dieter Schnabel, Ralf Paschinger, Katharina Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title | Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title_full | Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title_fullStr | Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title_full_unstemmed | Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title_short | Increasing Complexity of the N-Glycome During Caenorhabditis Development |
title_sort | increasing complexity of the n-glycome during caenorhabditis development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614267/ https://www.ncbi.nlm.nih.gov/pubmed/36717059 http://dx.doi.org/10.1016/j.mcpro.2023.100505 |
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