Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes

The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH s...

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Autores principales: Capitani, Nagaja, Cassioli, Chiara, Ravichandran, Keerthana, Baldari, Cosima T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614623/
https://www.ncbi.nlm.nih.gov/pubmed/37106198
http://dx.doi.org/10.1007/978-1-0716-3135-5_27
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author Capitani, Nagaja
Cassioli, Chiara
Ravichandran, Keerthana
Baldari, Cosima T.
author_facet Capitani, Nagaja
Cassioli, Chiara
Ravichandran, Keerthana
Baldari, Cosima T.
author_sort Capitani, Nagaja
collection PubMed
description The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.
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spelling pubmed-76146232023-06-06 Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes Capitani, Nagaja Cassioli, Chiara Ravichandran, Keerthana Baldari, Cosima T. Methods Mol Biol Article The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment. 2023-01-01 /pmc/articles/PMC7614623/ /pubmed/37106198 http://dx.doi.org/10.1007/978-1-0716-3135-5_27 Text en https://creativecommons.org/licenses/by/4.0/This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made. The images or other third party material in this chapter are included in the chapter’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the chapter’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
spellingShingle Article
Capitani, Nagaja
Cassioli, Chiara
Ravichandran, Keerthana
Baldari, Cosima T.
Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title_full Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title_fullStr Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title_full_unstemmed Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title_short Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes
title_sort exploiting the rush system to study lytic granule biogenesis in cytotoxic t lymphocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7614623/
https://www.ncbi.nlm.nih.gov/pubmed/37106198
http://dx.doi.org/10.1007/978-1-0716-3135-5_27
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AT ravichandrankeerthana exploitingtherushsystemtostudylyticgranulebiogenesisincytotoxictlymphocytes
AT baldaricosimat exploitingtherushsystemtostudylyticgranulebiogenesisincytotoxictlymphocytes

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