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Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy
[Image: see text] Fusion of biological membranes is fundamental in various physiological events. The fusion process involves several intermediate stages with energy barriers that are tightly dependent on the mechanical and physical properties of the system, one of which is membrane tension. As previ...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7615193/ https://www.ncbi.nlm.nih.gov/pubmed/37669531 http://dx.doi.org/10.1021/acsnano.3c04293 |
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author | Shendrik, Petr Golani, Gonen Dharan, Raviv Schwarz, Ulrich S. Sorkin, Raya |
author_facet | Shendrik, Petr Golani, Gonen Dharan, Raviv Schwarz, Ulrich S. Sorkin, Raya |
author_sort | Shendrik, Petr |
collection | PubMed |
description | [Image: see text] Fusion of biological membranes is fundamental in various physiological events. The fusion process involves several intermediate stages with energy barriers that are tightly dependent on the mechanical and physical properties of the system, one of which is membrane tension. As previously established, the late stages of fusion, including hemifusion diaphragm and pore expansions, are favored by membrane tension. However, a current understanding of how the energy barrier of earlier fusion stages is affected by membrane tension is lacking. Here, we apply a newly developed experimental approach combining micropipette-aspirated giant unilamellar vesicles and optically trapped membrane-coated beads, revealing that membrane tension inhibits lipid mixing. We show that lipid mixing is 6 times slower under a tension of 0.12 mN/m compared with tension-free membranes. Furthermore, using continuum elastic theory, we calculate the dependence of the hemifusion stalk formation energy on membrane tension and intermembrane distance and find the increase in the corresponding energy barrier to be 1.6 k(B)T in our setting, which can explain the increase in lipid mixing time delay. Finally, we show that tension can be a significant factor in the stalk energy if the pre-fusion intermembrane distance is on the order of several nanometers, while for membranes that are tightly docked, tension has a negligible effect. |
format | Online Article Text |
id | pubmed-7615193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-76151932023-10-11 Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy Shendrik, Petr Golani, Gonen Dharan, Raviv Schwarz, Ulrich S. Sorkin, Raya ACS Nano [Image: see text] Fusion of biological membranes is fundamental in various physiological events. The fusion process involves several intermediate stages with energy barriers that are tightly dependent on the mechanical and physical properties of the system, one of which is membrane tension. As previously established, the late stages of fusion, including hemifusion diaphragm and pore expansions, are favored by membrane tension. However, a current understanding of how the energy barrier of earlier fusion stages is affected by membrane tension is lacking. Here, we apply a newly developed experimental approach combining micropipette-aspirated giant unilamellar vesicles and optically trapped membrane-coated beads, revealing that membrane tension inhibits lipid mixing. We show that lipid mixing is 6 times slower under a tension of 0.12 mN/m compared with tension-free membranes. Furthermore, using continuum elastic theory, we calculate the dependence of the hemifusion stalk formation energy on membrane tension and intermembrane distance and find the increase in the corresponding energy barrier to be 1.6 k(B)T in our setting, which can explain the increase in lipid mixing time delay. Finally, we show that tension can be a significant factor in the stalk energy if the pre-fusion intermembrane distance is on the order of several nanometers, while for membranes that are tightly docked, tension has a negligible effect. American Chemical Society 2023-09-05 /pmc/articles/PMC7615193/ /pubmed/37669531 http://dx.doi.org/10.1021/acsnano.3c04293 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Shendrik, Petr Golani, Gonen Dharan, Raviv Schwarz, Ulrich S. Sorkin, Raya Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy |
title | Membrane
Tension Inhibits Lipid Mixing by Increasing
the Hemifusion Stalk Energy |
title_full | Membrane
Tension Inhibits Lipid Mixing by Increasing
the Hemifusion Stalk Energy |
title_fullStr | Membrane
Tension Inhibits Lipid Mixing by Increasing
the Hemifusion Stalk Energy |
title_full_unstemmed | Membrane
Tension Inhibits Lipid Mixing by Increasing
the Hemifusion Stalk Energy |
title_short | Membrane
Tension Inhibits Lipid Mixing by Increasing
the Hemifusion Stalk Energy |
title_sort | membrane
tension inhibits lipid mixing by increasing
the hemifusion stalk energy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7615193/ https://www.ncbi.nlm.nih.gov/pubmed/37669531 http://dx.doi.org/10.1021/acsnano.3c04293 |
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