Cargando…
Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells
Although mitochondrial functions are essential for cell survival, their critical roles in stem cell fate, including proliferation, differentiation, and senescence, remain elusive. Ginsenoside Rg3 exhibits various biological activities and reportedly increases mitochondrial biogenesis and respiration...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640456/ https://www.ncbi.nlm.nih.gov/pubmed/33148318 http://dx.doi.org/10.1186/s13287-020-01974-3 |
_version_ | 1783605751341645824 |
---|---|
author | Hong, Taeui Kim, Moon Young Da Ly, Dat Park, Su Jung Eom, Young Woo Park, Kyu-Sang Baik, Soon Koo |
author_facet | Hong, Taeui Kim, Moon Young Da Ly, Dat Park, Su Jung Eom, Young Woo Park, Kyu-Sang Baik, Soon Koo |
author_sort | Hong, Taeui |
collection | PubMed |
description | Although mitochondrial functions are essential for cell survival, their critical roles in stem cell fate, including proliferation, differentiation, and senescence, remain elusive. Ginsenoside Rg3 exhibits various biological activities and reportedly increases mitochondrial biogenesis and respiration. Herein, we observed that Rg3 increased proliferation and suppressed senescence of human bone marrow-derived mesenchymal stem cells. Osteogenic, but not adipogenic, differentiation was facilitated by Rg3 treatment. Rg3 suppressed reactive oxygen species production and upregulated mitochondrial biogenesis and antioxidant enzymes, including superoxide dismutase. Consistently, Rg3 strongly augmented basal and ATP synthesis-linked respiration with high spare respiratory capacity. Rg3 treatment elevated cytosolic Ca(2+) concentration contributing to mitochondrial activation. Reduction of intracellular or extracellular Ca(2+) levels strongly inhibited Rg3-induced activation of mitochondrial respiration and biogenesis. Taken together, Rg3 enhances capabilities of mitochondrial and antioxidant functions mainly through a Ca(2+)-dependent pathway, which improves the proliferation and differentiation potentials and prevents the senescence of human mesenchymal stem cells. |
format | Online Article Text |
id | pubmed-7640456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76404562020-11-04 Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells Hong, Taeui Kim, Moon Young Da Ly, Dat Park, Su Jung Eom, Young Woo Park, Kyu-Sang Baik, Soon Koo Stem Cell Res Ther Short Report Although mitochondrial functions are essential for cell survival, their critical roles in stem cell fate, including proliferation, differentiation, and senescence, remain elusive. Ginsenoside Rg3 exhibits various biological activities and reportedly increases mitochondrial biogenesis and respiration. Herein, we observed that Rg3 increased proliferation and suppressed senescence of human bone marrow-derived mesenchymal stem cells. Osteogenic, but not adipogenic, differentiation was facilitated by Rg3 treatment. Rg3 suppressed reactive oxygen species production and upregulated mitochondrial biogenesis and antioxidant enzymes, including superoxide dismutase. Consistently, Rg3 strongly augmented basal and ATP synthesis-linked respiration with high spare respiratory capacity. Rg3 treatment elevated cytosolic Ca(2+) concentration contributing to mitochondrial activation. Reduction of intracellular or extracellular Ca(2+) levels strongly inhibited Rg3-induced activation of mitochondrial respiration and biogenesis. Taken together, Rg3 enhances capabilities of mitochondrial and antioxidant functions mainly through a Ca(2+)-dependent pathway, which improves the proliferation and differentiation potentials and prevents the senescence of human mesenchymal stem cells. BioMed Central 2020-11-04 /pmc/articles/PMC7640456/ /pubmed/33148318 http://dx.doi.org/10.1186/s13287-020-01974-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Short Report Hong, Taeui Kim, Moon Young Da Ly, Dat Park, Su Jung Eom, Young Woo Park, Kyu-Sang Baik, Soon Koo Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title | Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title_full | Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title_fullStr | Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title_full_unstemmed | Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title_short | Ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in Rg3-treated human mesenchymal stem cells |
title_sort | ca(2+)-activated mitochondrial biogenesis and functions improve stem cell fate in rg3-treated human mesenchymal stem cells |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640456/ https://www.ncbi.nlm.nih.gov/pubmed/33148318 http://dx.doi.org/10.1186/s13287-020-01974-3 |
work_keys_str_mv | AT hongtaeui ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT kimmoonyoung ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT dalydat ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT parksujung ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT eomyoungwoo ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT parkkyusang ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells AT baiksoonkoo ca2activatedmitochondrialbiogenesisandfunctionsimprovestemcellfateinrg3treatedhumanmesenchymalstemcells |