Cargando…

Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)

BACKGROUND: Tartary buckwheat has gained popularity in the food marketplace due to its abundant nutrients and high bioactive flavonoid content. However, its difficult dehulling process has severely restricted its food processing industry development. Rice-tartary buckwheat, a rare local variety, is...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Hong-You, Wu, Chao-Xin, Lv, Qiu-Yu, Shi, Tao-Xiong, Chen, Qi-Jiao, Chen, Qing-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640676/
https://www.ncbi.nlm.nih.gov/pubmed/33148168
http://dx.doi.org/10.1186/s12870-020-02715-7
_version_ 1783605796759666688
author Li, Hong-You
Wu, Chao-Xin
Lv, Qiu-Yu
Shi, Tao-Xiong
Chen, Qi-Jiao
Chen, Qing-Fu
author_facet Li, Hong-You
Wu, Chao-Xin
Lv, Qiu-Yu
Shi, Tao-Xiong
Chen, Qi-Jiao
Chen, Qing-Fu
author_sort Li, Hong-You
collection PubMed
description BACKGROUND: Tartary buckwheat has gained popularity in the food marketplace due to its abundant nutrients and high bioactive flavonoid content. However, its difficult dehulling process has severely restricted its food processing industry development. Rice-tartary buckwheat, a rare local variety, is very easily dehulled, but the cellular, physiological and molecular mechanisms responsible for this easy dehulling remains largely unclear. RESULTS: In this study, we integrated analyses of the comparative cellular, physiological, transcriptome, and gene coexpression network to insight into the reason that rice-tartary buckwheat is easy to dehull. Compared to normal tartary buckwheat, rice-tartary buckwheat has significantly brittler and thinner hull, and thinner cell wall in hull sclerenchyma cells. Furthermore, the cellulose, hemicellulose, and lignin contents of rice-tartary buckwheat hull were significantly lower than those in all or part of the tested normal tartary buckwheat cultivars, respectively, and the significant difference in cellulose and hemicellulose contents between rice-tartary buckwheat and normal tartary buckwheat began at 10 days after pollination (DAP). Comparative transcriptome analysis identified a total of 9250 differentially expressed genes (DEGs) between the rice- and normal-tartary buckwheat hulls at four different development stages. Weighted gene coexpression network analysis (WGCNA) of all DEGs identified a key module associated with the formation of the hull difference between rice- and normal-tartary buckwheat. In this specific module, many secondary cell wall (SCW) biosynthesis regulatory and structural genes, which involved in cellulose and hemicellulose biosynthesis, were identified as hub genes and displayed coexpression. These identified hub genes of SCW biosynthesis were significantly lower expression in rice-tartary buckwheat hull than in normal tartary buckwheat at the early hull development stages. Among them, the expression of 17 SCW biosynthesis relative-hub genes were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). CONCLUSIONS: Our results showed that the lower expression of SCW biosynthesis regulatory and structural genes in rice-tartary buckwheat hull in the early development stages contributes to its easy dehulling by reducing the content of cell wall chemical components, which further effects the cell wall thickness of hull sclerenchyma cells, and hull thickness and mechanical strength. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12870-020-02715-7.
format Online
Article
Text
id pubmed-7640676
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-76406762020-11-04 Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum) Li, Hong-You Wu, Chao-Xin Lv, Qiu-Yu Shi, Tao-Xiong Chen, Qi-Jiao Chen, Qing-Fu BMC Plant Biol Research Article BACKGROUND: Tartary buckwheat has gained popularity in the food marketplace due to its abundant nutrients and high bioactive flavonoid content. However, its difficult dehulling process has severely restricted its food processing industry development. Rice-tartary buckwheat, a rare local variety, is very easily dehulled, but the cellular, physiological and molecular mechanisms responsible for this easy dehulling remains largely unclear. RESULTS: In this study, we integrated analyses of the comparative cellular, physiological, transcriptome, and gene coexpression network to insight into the reason that rice-tartary buckwheat is easy to dehull. Compared to normal tartary buckwheat, rice-tartary buckwheat has significantly brittler and thinner hull, and thinner cell wall in hull sclerenchyma cells. Furthermore, the cellulose, hemicellulose, and lignin contents of rice-tartary buckwheat hull were significantly lower than those in all or part of the tested normal tartary buckwheat cultivars, respectively, and the significant difference in cellulose and hemicellulose contents between rice-tartary buckwheat and normal tartary buckwheat began at 10 days after pollination (DAP). Comparative transcriptome analysis identified a total of 9250 differentially expressed genes (DEGs) between the rice- and normal-tartary buckwheat hulls at four different development stages. Weighted gene coexpression network analysis (WGCNA) of all DEGs identified a key module associated with the formation of the hull difference between rice- and normal-tartary buckwheat. In this specific module, many secondary cell wall (SCW) biosynthesis regulatory and structural genes, which involved in cellulose and hemicellulose biosynthesis, were identified as hub genes and displayed coexpression. These identified hub genes of SCW biosynthesis were significantly lower expression in rice-tartary buckwheat hull than in normal tartary buckwheat at the early hull development stages. Among them, the expression of 17 SCW biosynthesis relative-hub genes were further verified by quantitative real-time polymerase chain reaction (qRT-PCR). CONCLUSIONS: Our results showed that the lower expression of SCW biosynthesis regulatory and structural genes in rice-tartary buckwheat hull in the early development stages contributes to its easy dehulling by reducing the content of cell wall chemical components, which further effects the cell wall thickness of hull sclerenchyma cells, and hull thickness and mechanical strength. SUPPLEMENTARY INFORMATION: Supplementary information accompanies this paper at 10.1186/s12870-020-02715-7. BioMed Central 2020-11-04 /pmc/articles/PMC7640676/ /pubmed/33148168 http://dx.doi.org/10.1186/s12870-020-02715-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Li, Hong-You
Wu, Chao-Xin
Lv, Qiu-Yu
Shi, Tao-Xiong
Chen, Qi-Jiao
Chen, Qing-Fu
Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title_full Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title_fullStr Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title_full_unstemmed Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title_short Comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (Fagopyrum Tararicum)
title_sort comparative cellular, physiological and transcriptome analyses reveal the potential easy dehulling mechanism of rice-tartary buckwheat (fagopyrum tararicum)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640676/
https://www.ncbi.nlm.nih.gov/pubmed/33148168
http://dx.doi.org/10.1186/s12870-020-02715-7
work_keys_str_mv AT lihongyou comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum
AT wuchaoxin comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum
AT lvqiuyu comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum
AT shitaoxiong comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum
AT chenqijiao comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum
AT chenqingfu comparativecellularphysiologicalandtranscriptomeanalysesrevealthepotentialeasydehullingmechanismofricetartarybuckwheatfagopyrumtararicum