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Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer
Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, a...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640888/ https://www.ncbi.nlm.nih.gov/pubmed/33248470 http://dx.doi.org/10.1016/j.cell.2020.10.049 |
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author | Avanzato, Victoria A. Matson, M. Jeremiah Seifert, Stephanie N. Pryce, Rhys Williamson, Brandi N. Anzick, Sarah L. Barbian, Kent Judson, Seth D. Fischer, Elizabeth R. Martens, Craig Bowden, Thomas A. de Wit, Emmie Riedo, Francis X. Munster, Vincent J. |
author_facet | Avanzato, Victoria A. Matson, M. Jeremiah Seifert, Stephanie N. Pryce, Rhys Williamson, Brandi N. Anzick, Sarah L. Barbian, Kent Judson, Seth D. Fischer, Elizabeth R. Martens, Craig Bowden, Thomas A. de Wit, Emmie Riedo, Francis X. Munster, Vincent J. |
author_sort | Avanzato, Victoria A. |
collection | PubMed |
description | Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and subgenomic RNA up to 105 days, after initial diagnosis. The infection was not cleared after the first treatment with convalescent plasma, suggesting a limited effect on SARS-CoV-2 in the upper respiratory tract of this individual. Several weeks after a second convalescent plasma transfusion, SARS-CoV-2 RNA was no longer detected. We observed marked within-host genomic evolution of SARS-CoV-2 with continuous turnover of dominant viral variants. However, replication kinetics in Vero E6 cells and primary human alveolar epithelial tissues were not affected. Our data indicate that certain immunocompromised individuals may shed infectious virus longer than previously recognized. Detection of subgenomic RNA is recommended in persistently SARS-CoV-2-positive individuals as a proxy for shedding of infectious virus. |
format | Online Article Text |
id | pubmed-7640888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76408882020-11-05 Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer Avanzato, Victoria A. Matson, M. Jeremiah Seifert, Stephanie N. Pryce, Rhys Williamson, Brandi N. Anzick, Sarah L. Barbian, Kent Judson, Seth D. Fischer, Elizabeth R. Martens, Craig Bowden, Thomas A. de Wit, Emmie Riedo, Francis X. Munster, Vincent J. Cell Article Long-term severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding was observed from the upper respiratory tract of a female immunocompromised individual with chronic lymphocytic leukemia and acquired hypogammaglobulinemia. Shedding of infectious SARS-CoV-2 was observed up to 70 days, and of genomic and subgenomic RNA up to 105 days, after initial diagnosis. The infection was not cleared after the first treatment with convalescent plasma, suggesting a limited effect on SARS-CoV-2 in the upper respiratory tract of this individual. Several weeks after a second convalescent plasma transfusion, SARS-CoV-2 RNA was no longer detected. We observed marked within-host genomic evolution of SARS-CoV-2 with continuous turnover of dominant viral variants. However, replication kinetics in Vero E6 cells and primary human alveolar epithelial tissues were not affected. Our data indicate that certain immunocompromised individuals may shed infectious virus longer than previously recognized. Detection of subgenomic RNA is recommended in persistently SARS-CoV-2-positive individuals as a proxy for shedding of infectious virus. Cell Press 2020-12-23 2020-11-04 /pmc/articles/PMC7640888/ /pubmed/33248470 http://dx.doi.org/10.1016/j.cell.2020.10.049 Text en Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Avanzato, Victoria A. Matson, M. Jeremiah Seifert, Stephanie N. Pryce, Rhys Williamson, Brandi N. Anzick, Sarah L. Barbian, Kent Judson, Seth D. Fischer, Elizabeth R. Martens, Craig Bowden, Thomas A. de Wit, Emmie Riedo, Francis X. Munster, Vincent J. Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title | Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title_full | Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title_fullStr | Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title_full_unstemmed | Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title_short | Case Study: Prolonged Infectious SARS-CoV-2 Shedding from an Asymptomatic Immunocompromised Individual with Cancer |
title_sort | case study: prolonged infectious sars-cov-2 shedding from an asymptomatic immunocompromised individual with cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640888/ https://www.ncbi.nlm.nih.gov/pubmed/33248470 http://dx.doi.org/10.1016/j.cell.2020.10.049 |
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