METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue

Mammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m(4)C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m(4)Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse...

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Autores principales: Laptev, Ivan, Shvetsova, Ekaterina, Levitskii, Sergey, Serebryakova, Marina, Rubtsova, Maria, Zgoda, Victor, Bogdanov, Alexey, Kamenski, Piotr, Sergiev, Petr, Dontsova, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641309/
https://www.ncbi.nlm.nih.gov/pubmed/32573735
http://dx.doi.org/10.1093/nar/gkaa522
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author Laptev, Ivan
Shvetsova, Ekaterina
Levitskii, Sergey
Serebryakova, Marina
Rubtsova, Maria
Zgoda, Victor
Bogdanov, Alexey
Kamenski, Piotr
Sergiev, Petr
Dontsova, Olga
author_facet Laptev, Ivan
Shvetsova, Ekaterina
Levitskii, Sergey
Serebryakova, Marina
Rubtsova, Maria
Zgoda, Victor
Bogdanov, Alexey
Kamenski, Piotr
Sergiev, Petr
Dontsova, Olga
author_sort Laptev, Ivan
collection PubMed
description Mammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m(4)C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m(4)Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse METTL15 protein is responsible for the formation of m(4)C residue of the 12S rRNA. Inactivation of Mettl15 gene in murine cell line perturbs the composition of mitochondrial protein biosynthesis machinery. Identification of METTL15 interaction partners revealed that the likely substrate for this RNA methyltransferase is an assembly intermediate of the mitochondrial small ribosomal subunit containing an assembly factor RBFA.
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spelling pubmed-76413092020-11-10 METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue Laptev, Ivan Shvetsova, Ekaterina Levitskii, Sergey Serebryakova, Marina Rubtsova, Maria Zgoda, Victor Bogdanov, Alexey Kamenski, Piotr Sergiev, Petr Dontsova, Olga Nucleic Acids Res RNA and RNA-protein complexes Mammalian mitochondrial ribosomes contain a set of modified nucleotides, which is distinct from that of the cytosolic ribosomes. Nucleotide m(4)C840 of the murine mitochondrial 12S rRNA is equivalent to the dimethylated m(4)Cm1402 residue of Escherichia coli 16S rRNA. Here we demonstrate that mouse METTL15 protein is responsible for the formation of m(4)C residue of the 12S rRNA. Inactivation of Mettl15 gene in murine cell line perturbs the composition of mitochondrial protein biosynthesis machinery. Identification of METTL15 interaction partners revealed that the likely substrate for this RNA methyltransferase is an assembly intermediate of the mitochondrial small ribosomal subunit containing an assembly factor RBFA. Oxford University Press 2020-06-23 /pmc/articles/PMC7641309/ /pubmed/32573735 http://dx.doi.org/10.1093/nar/gkaa522 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA and RNA-protein complexes
Laptev, Ivan
Shvetsova, Ekaterina
Levitskii, Sergey
Serebryakova, Marina
Rubtsova, Maria
Zgoda, Victor
Bogdanov, Alexey
Kamenski, Piotr
Sergiev, Petr
Dontsova, Olga
METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title_full METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title_fullStr METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title_full_unstemmed METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title_short METTL15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)C840 12S rRNA residue
title_sort mettl15 interacts with the assembly intermediate of murine mitochondrial small ribosomal subunit to form m(4)c840 12s rrna residue
topic RNA and RNA-protein complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7641309/
https://www.ncbi.nlm.nih.gov/pubmed/32573735
http://dx.doi.org/10.1093/nar/gkaa522
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