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Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil

The human T-lymphotropic virus (HTLV) is part of the group of retroviruses that share similar routes of transmission to the human immunodeficiency virus (HIV). Coinfection of these viruses can affect the clinical course of both infections, and reports have shown a quicker progression to AIDS and the...

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Autores principales: Alencar, Samira Peixoto, Souza, Marlinda de Carvalho, Fonseca, Ricardo Roberto de Souza, Menezes, Cláudia Ribeiro, Azevedo, Vânia Nakauth, Ribeiro, Andre Luis Ribeiro, Lima, Sandra Souza, Laurentino, Rogério Valois, Barbosa, Maria dos Anjos de Abreu Pina, Freitas, Felipe Bonfim, Oliveira-Filho, Aldemir Branco, Machado, Luiz Fernando Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642294/
https://www.ncbi.nlm.nih.gov/pubmed/33193170
http://dx.doi.org/10.3389/fmicb.2020.572381
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author Alencar, Samira Peixoto
Souza, Marlinda de Carvalho
Fonseca, Ricardo Roberto de Souza
Menezes, Cláudia Ribeiro
Azevedo, Vânia Nakauth
Ribeiro, Andre Luis Ribeiro
Lima, Sandra Souza
Laurentino, Rogério Valois
Barbosa, Maria dos Anjos de Abreu Pina
Freitas, Felipe Bonfim
Oliveira-Filho, Aldemir Branco
Machado, Luiz Fernando Almeida
author_facet Alencar, Samira Peixoto
Souza, Marlinda de Carvalho
Fonseca, Ricardo Roberto de Souza
Menezes, Cláudia Ribeiro
Azevedo, Vânia Nakauth
Ribeiro, Andre Luis Ribeiro
Lima, Sandra Souza
Laurentino, Rogério Valois
Barbosa, Maria dos Anjos de Abreu Pina
Freitas, Felipe Bonfim
Oliveira-Filho, Aldemir Branco
Machado, Luiz Fernando Almeida
author_sort Alencar, Samira Peixoto
collection PubMed
description The human T-lymphotropic virus (HTLV) is part of the group of retroviruses that share similar routes of transmission to the human immunodeficiency virus (HIV). Coinfection of these viruses can affect the clinical course of both infections, and reports have shown a quicker progression to AIDS and the development of HIV-related opportunistic infections. The current study investigated the demographic characteristics, prevalence, and the subtypes of HTLV among people living with HIV/AIDS (PLWHA) in the State of Pará, Northern Brazil. Blood samples were obtained from patients who were attending a reference unit that provides medical assistance to HIV-infected individuals in the State of Pará, Brazil, during the period of May 2016 to June 2017. Plasma samples were screened by ELISA tests to detect antibodies anti-HTLV-1/2. DNA and viral types were identified by real-time polymerase chain reaction (qPCR). All samples with viral DNA were submitted to nested PCR and nucleotide sequencing. The overall coinfection rate was 1.4% (5/368), and all samples were from subtype HTLV-1a. No cases of HTLV-2 infection were detected. The prevalence of HTLV-1 was higher in females (80%), individuals between 31 and 50 years of age, heterosexual, unmarried, with low monthly income, with secondary educational level or higher, sporadic condom usage, limited number of sexual partners, and no history of sexually transmitted infections. All samples from HTLV-1-infected patients were identified as strains belonging to the subtype 1a (Cosmopolitan), subgroup A (Transcontinental). This study identified that the prevalence of HIV/HTLV coinfection has dropped from 8 to 1.3% in the current investigation. There was a shift of HTLV subtype from a predominance of HTLV-2 infection in the past to an actual exclusively HTLV-1a. There was no significant association between economic, sociodemographic, and behavioral characteristics in HIV/HTLV coinfection.
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spelling pubmed-76422942020-11-13 Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil Alencar, Samira Peixoto Souza, Marlinda de Carvalho Fonseca, Ricardo Roberto de Souza Menezes, Cláudia Ribeiro Azevedo, Vânia Nakauth Ribeiro, Andre Luis Ribeiro Lima, Sandra Souza Laurentino, Rogério Valois Barbosa, Maria dos Anjos de Abreu Pina Freitas, Felipe Bonfim Oliveira-Filho, Aldemir Branco Machado, Luiz Fernando Almeida Front Microbiol Microbiology The human T-lymphotropic virus (HTLV) is part of the group of retroviruses that share similar routes of transmission to the human immunodeficiency virus (HIV). Coinfection of these viruses can affect the clinical course of both infections, and reports have shown a quicker progression to AIDS and the development of HIV-related opportunistic infections. The current study investigated the demographic characteristics, prevalence, and the subtypes of HTLV among people living with HIV/AIDS (PLWHA) in the State of Pará, Northern Brazil. Blood samples were obtained from patients who were attending a reference unit that provides medical assistance to HIV-infected individuals in the State of Pará, Brazil, during the period of May 2016 to June 2017. Plasma samples were screened by ELISA tests to detect antibodies anti-HTLV-1/2. DNA and viral types were identified by real-time polymerase chain reaction (qPCR). All samples with viral DNA were submitted to nested PCR and nucleotide sequencing. The overall coinfection rate was 1.4% (5/368), and all samples were from subtype HTLV-1a. No cases of HTLV-2 infection were detected. The prevalence of HTLV-1 was higher in females (80%), individuals between 31 and 50 years of age, heterosexual, unmarried, with low monthly income, with secondary educational level or higher, sporadic condom usage, limited number of sexual partners, and no history of sexually transmitted infections. All samples from HTLV-1-infected patients were identified as strains belonging to the subtype 1a (Cosmopolitan), subgroup A (Transcontinental). This study identified that the prevalence of HIV/HTLV coinfection has dropped from 8 to 1.3% in the current investigation. There was a shift of HTLV subtype from a predominance of HTLV-2 infection in the past to an actual exclusively HTLV-1a. There was no significant association between economic, sociodemographic, and behavioral characteristics in HIV/HTLV coinfection. Frontiers Media S.A. 2020-10-22 /pmc/articles/PMC7642294/ /pubmed/33193170 http://dx.doi.org/10.3389/fmicb.2020.572381 Text en Copyright © 2020 Alencar, Souza, Fonseca, Menezes, Azevedo, Ribeiro, Lima, Laurentino, Barbosa, Freitas, Oliveira-Filho and Machado. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Alencar, Samira Peixoto
Souza, Marlinda de Carvalho
Fonseca, Ricardo Roberto de Souza
Menezes, Cláudia Ribeiro
Azevedo, Vânia Nakauth
Ribeiro, Andre Luis Ribeiro
Lima, Sandra Souza
Laurentino, Rogério Valois
Barbosa, Maria dos Anjos de Abreu Pina
Freitas, Felipe Bonfim
Oliveira-Filho, Aldemir Branco
Machado, Luiz Fernando Almeida
Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title_full Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title_fullStr Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title_full_unstemmed Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title_short Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil
title_sort prevalence and molecular epidemiology of human t-lymphotropic virus (htlv) infection in people living with hiv/aids in the pará state, amazon region of brazil
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642294/
https://www.ncbi.nlm.nih.gov/pubmed/33193170
http://dx.doi.org/10.3389/fmicb.2020.572381
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