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The persistence potential of transferable plasmids

Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid’s ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the conditions unde...

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Detalles Bibliográficos
Autores principales: Wang, Teng, You, Lingchong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642394/
https://www.ncbi.nlm.nih.gov/pubmed/33149119
http://dx.doi.org/10.1038/s41467-020-19368-7
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author Wang, Teng
You, Lingchong
author_facet Wang, Teng
You, Lingchong
author_sort Wang, Teng
collection PubMed
description Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid’s ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the conditions underlying plasmid persistence are poorly understood. Here, we describe a plasmid-centric framework that makes it computationally feasible to analyze gene flow in complex communities. Using this framework, we derive the ‘persistence potential’: a general, heuristic metric that predicts the persistence and abundance of any plasmids. We validate the metric with engineered microbial consortia transferring mobilizable plasmids and with quantitative data available in the literature. We believe that our framework and the resulting metric will facilitate a quantitative understanding of natural microbial communities and the engineering of microbial consortia.
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spelling pubmed-76423942020-11-10 The persistence potential of transferable plasmids Wang, Teng You, Lingchong Nat Commun Article Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid’s ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the conditions underlying plasmid persistence are poorly understood. Here, we describe a plasmid-centric framework that makes it computationally feasible to analyze gene flow in complex communities. Using this framework, we derive the ‘persistence potential’: a general, heuristic metric that predicts the persistence and abundance of any plasmids. We validate the metric with engineered microbial consortia transferring mobilizable plasmids and with quantitative data available in the literature. We believe that our framework and the resulting metric will facilitate a quantitative understanding of natural microbial communities and the engineering of microbial consortia. Nature Publishing Group UK 2020-11-04 /pmc/articles/PMC7642394/ /pubmed/33149119 http://dx.doi.org/10.1038/s41467-020-19368-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Teng
You, Lingchong
The persistence potential of transferable plasmids
title The persistence potential of transferable plasmids
title_full The persistence potential of transferable plasmids
title_fullStr The persistence potential of transferable plasmids
title_full_unstemmed The persistence potential of transferable plasmids
title_short The persistence potential of transferable plasmids
title_sort persistence potential of transferable plasmids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7642394/
https://www.ncbi.nlm.nih.gov/pubmed/33149119
http://dx.doi.org/10.1038/s41467-020-19368-7
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