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MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches

Tiny Earth (TE) is a popular international citizen science program aimed at improving public awareness on the growing antimicrobial resistance problem of which MicroMundo Albacete is a Spanish node. With a protocol that is focused on the isolation of antibiotics-producing actinomycetes from soil, 70...

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Autores principales: Alvarado, María, Clemente-Casares, Pilar, Moreno, Diego A., de Groot, Piet W. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643607/
https://www.ncbi.nlm.nih.gov/pubmed/33193197
http://dx.doi.org/10.3389/fmicb.2020.577550
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author Alvarado, María
Clemente-Casares, Pilar
Moreno, Diego A.
de Groot, Piet W. J.
author_facet Alvarado, María
Clemente-Casares, Pilar
Moreno, Diego A.
de Groot, Piet W. J.
author_sort Alvarado, María
collection PubMed
description Tiny Earth (TE) is a popular international citizen science program aimed at improving public awareness on the growing antimicrobial resistance problem of which MicroMundo Albacete is a Spanish node. With a protocol that is focused on the isolation of antibiotics-producing actinomycetes from soil, 70% of the high school students in MicroMundo Albacete 2020 isolated colonies with antagonistic activity against Gram-positive tester bacteria. However, no activity was found against Gram-negative bacteria. Here, we further adapted the protocol toward a more targeted screening that also enables isolation of antagonistic bacteria against Gram negatives using two different reverse-antibiosis approaches involving a spraying technique or flipping soil sample disks upside down. Exploiting the soil samples from MicroMundo Albacete 2020, the new approaches yielded isolation of actinomycete bacteria with antagonistic activity against Gram-negative as well as Gram-positive tester bacteria. We propose that (educational) science programs which aim to search for antibiotic-producing bacteria may implement these approaches in their protocol to promote a successful and stimulating outcome of the experiment for the participating students.
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spelling pubmed-76436072020-11-13 MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches Alvarado, María Clemente-Casares, Pilar Moreno, Diego A. de Groot, Piet W. J. Front Microbiol Microbiology Tiny Earth (TE) is a popular international citizen science program aimed at improving public awareness on the growing antimicrobial resistance problem of which MicroMundo Albacete is a Spanish node. With a protocol that is focused on the isolation of antibiotics-producing actinomycetes from soil, 70% of the high school students in MicroMundo Albacete 2020 isolated colonies with antagonistic activity against Gram-positive tester bacteria. However, no activity was found against Gram-negative bacteria. Here, we further adapted the protocol toward a more targeted screening that also enables isolation of antagonistic bacteria against Gram negatives using two different reverse-antibiosis approaches involving a spraying technique or flipping soil sample disks upside down. Exploiting the soil samples from MicroMundo Albacete 2020, the new approaches yielded isolation of actinomycete bacteria with antagonistic activity against Gram-negative as well as Gram-positive tester bacteria. We propose that (educational) science programs which aim to search for antibiotic-producing bacteria may implement these approaches in their protocol to promote a successful and stimulating outcome of the experiment for the participating students. Frontiers Media S.A. 2020-10-15 /pmc/articles/PMC7643607/ /pubmed/33193197 http://dx.doi.org/10.3389/fmicb.2020.577550 Text en Copyright © 2020 Alvarado, Clemente-Casares, Moreno and de Groot. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Alvarado, María
Clemente-Casares, Pilar
Moreno, Diego A.
de Groot, Piet W. J.
MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title_full MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title_fullStr MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title_full_unstemmed MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title_short MicroMundo Upside Down: Targeted Searching for Antibiotics-Producing Bacteria From Soil With Reverse Antibiosis Approaches
title_sort micromundo upside down: targeted searching for antibiotics-producing bacteria from soil with reverse antibiosis approaches
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643607/
https://www.ncbi.nlm.nih.gov/pubmed/33193197
http://dx.doi.org/10.3389/fmicb.2020.577550
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