Cargando…
Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to diffe...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644756/ https://www.ncbi.nlm.nih.gov/pubmed/33154433 http://dx.doi.org/10.1038/s41598-020-75707-0 |
_version_ | 1783606519681515520 |
---|---|
author | Zhao, Lihan Rupji, Manali Choudhary, Ishita Osan, Remus Kapoor, Shobhna Zhang, Hong-Jie Yang, Chunhua Aneja, Ritu |
author_facet | Zhao, Lihan Rupji, Manali Choudhary, Ishita Osan, Remus Kapoor, Shobhna Zhang, Hong-Jie Yang, Chunhua Aneja, Ritu |
author_sort | Zhao, Lihan |
collection | PubMed |
description | Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC(50)) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC(50) values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts. |
format | Online Article Text |
id | pubmed-7644756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76447562020-11-06 Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer Zhao, Lihan Rupji, Manali Choudhary, Ishita Osan, Remus Kapoor, Shobhna Zhang, Hong-Jie Yang, Chunhua Aneja, Ritu Sci Rep Article Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC(50)) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC(50) values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts. Nature Publishing Group UK 2020-11-05 /pmc/articles/PMC7644756/ /pubmed/33154433 http://dx.doi.org/10.1038/s41598-020-75707-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhao, Lihan Rupji, Manali Choudhary, Ishita Osan, Remus Kapoor, Shobhna Zhang, Hong-Jie Yang, Chunhua Aneja, Ritu Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title | Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title_full | Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title_fullStr | Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title_full_unstemmed | Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title_short | Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
title_sort | efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644756/ https://www.ncbi.nlm.nih.gov/pubmed/33154433 http://dx.doi.org/10.1038/s41598-020-75707-0 |
work_keys_str_mv | AT zhaolihan efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT rupjimanali efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT choudharyishita efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT osanremus efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT kapoorshobhna efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT zhanghongjie efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT yangchunhua efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer AT anejaritu efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer |