Cargando…

Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer

Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Lihan, Rupji, Manali, Choudhary, Ishita, Osan, Remus, Kapoor, Shobhna, Zhang, Hong-Jie, Yang, Chunhua, Aneja, Ritu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644756/
https://www.ncbi.nlm.nih.gov/pubmed/33154433
http://dx.doi.org/10.1038/s41598-020-75707-0
_version_ 1783606519681515520
author Zhao, Lihan
Rupji, Manali
Choudhary, Ishita
Osan, Remus
Kapoor, Shobhna
Zhang, Hong-Jie
Yang, Chunhua
Aneja, Ritu
author_facet Zhao, Lihan
Rupji, Manali
Choudhary, Ishita
Osan, Remus
Kapoor, Shobhna
Zhang, Hong-Jie
Yang, Chunhua
Aneja, Ritu
author_sort Zhao, Lihan
collection PubMed
description Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC(50)) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC(50) values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts.
format Online
Article
Text
id pubmed-7644756
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-76447562020-11-06 Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer Zhao, Lihan Rupji, Manali Choudhary, Ishita Osan, Remus Kapoor, Shobhna Zhang, Hong-Jie Yang, Chunhua Aneja, Ritu Sci Rep Article Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC(50)) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC(50) values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts. Nature Publishing Group UK 2020-11-05 /pmc/articles/PMC7644756/ /pubmed/33154433 http://dx.doi.org/10.1038/s41598-020-75707-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhao, Lihan
Rupji, Manali
Choudhary, Ishita
Osan, Remus
Kapoor, Shobhna
Zhang, Hong-Jie
Yang, Chunhua
Aneja, Ritu
Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title_full Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title_fullStr Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title_full_unstemmed Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title_short Efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
title_sort efficacy based ginger fingerprinting reveals potential antiproliferative analytes for triple negative breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644756/
https://www.ncbi.nlm.nih.gov/pubmed/33154433
http://dx.doi.org/10.1038/s41598-020-75707-0
work_keys_str_mv AT zhaolihan efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT rupjimanali efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT choudharyishita efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT osanremus efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT kapoorshobhna efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT zhanghongjie efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT yangchunhua efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer
AT anejaritu efficacybasedgingerfingerprintingrevealspotentialantiproliferativeanalytesfortriplenegativebreastcancer