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Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response
Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gen...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644771/ https://www.ncbi.nlm.nih.gov/pubmed/33154461 http://dx.doi.org/10.1038/s41598-020-75682-6 |
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author | Bensoussan, Nicolas Dixit, Sameer Tabara, Midori Letwin, David Milojevic, Maja Antonacci, Michele Jin, Pengyu Arai, Yuka Bruinsma, Kristie Suzuki, Takeshi Fukuhara, Toshiyuki Zhurov, Vladimir Geibel, Sven Nauen, Ralf Grbic, Miodrag Grbic, Vojislava |
author_facet | Bensoussan, Nicolas Dixit, Sameer Tabara, Midori Letwin, David Milojevic, Maja Antonacci, Michele Jin, Pengyu Arai, Yuka Bruinsma, Kristie Suzuki, Takeshi Fukuhara, Toshiyuki Zhurov, Vladimir Geibel, Sven Nauen, Ralf Grbic, Miodrag Grbic, Vojislava |
author_sort | Bensoussan, Nicolas |
collection | PubMed |
description | Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways. |
format | Online Article Text |
id | pubmed-7644771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76447712020-11-06 Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response Bensoussan, Nicolas Dixit, Sameer Tabara, Midori Letwin, David Milojevic, Maja Antonacci, Michele Jin, Pengyu Arai, Yuka Bruinsma, Kristie Suzuki, Takeshi Fukuhara, Toshiyuki Zhurov, Vladimir Geibel, Sven Nauen, Ralf Grbic, Miodrag Grbic, Vojislava Sci Rep Article Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAi-based pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways. Nature Publishing Group UK 2020-11-05 /pmc/articles/PMC7644771/ /pubmed/33154461 http://dx.doi.org/10.1038/s41598-020-75682-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Bensoussan, Nicolas Dixit, Sameer Tabara, Midori Letwin, David Milojevic, Maja Antonacci, Michele Jin, Pengyu Arai, Yuka Bruinsma, Kristie Suzuki, Takeshi Fukuhara, Toshiyuki Zhurov, Vladimir Geibel, Sven Nauen, Ralf Grbic, Miodrag Grbic, Vojislava Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title | Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title_full | Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title_fullStr | Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title_full_unstemmed | Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title_short | Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response |
title_sort | environmental rna interference in two-spotted spider mite, tetranychus urticae, reveals dsrna processing requirements for efficient rnai response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644771/ https://www.ncbi.nlm.nih.gov/pubmed/33154461 http://dx.doi.org/10.1038/s41598-020-75682-6 |
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