G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2

The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is one of seven human coronaviruses. G-quadruplexes are intrinsic obstacles to genome replication. Whether G-quadruplexes are present in human coronaviruses is unk...

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Detalles Bibliográficos
Autores principales: Cui, Haoran, Zhang, Leiliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644843/
https://www.ncbi.nlm.nih.gov/pubmed/33193156
http://dx.doi.org/10.3389/fmicb.2020.567317
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author Cui, Haoran
Zhang, Leiliang
author_facet Cui, Haoran
Zhang, Leiliang
author_sort Cui, Haoran
collection PubMed
description The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is one of seven human coronaviruses. G-quadruplexes are intrinsic obstacles to genome replication. Whether G-quadruplexes are present in human coronaviruses is unknown. In the current study, we have predicted that all seven human coronaviruses harbor G-quadruplex sequences. Conserved G-quadruplex sequences in SARS-CoV and SARS-CoV-2 were analyzed and verified by circular dichroism (CD) spectroscopy and Thioflavin T fluorescence assay. Similar to SARS-CoV, SARS-CoV-2 encodes an nsP3 protein, which is predicted to associate with G-quadruplexes. Targeting G-quadruplex sequences in the SARS-CoV-2 genome by G-quadruplex ligands could be a new way to conquer COVID-19.
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spelling pubmed-76448432020-11-13 G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2 Cui, Haoran Zhang, Leiliang Front Microbiol Microbiology The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is one of seven human coronaviruses. G-quadruplexes are intrinsic obstacles to genome replication. Whether G-quadruplexes are present in human coronaviruses is unknown. In the current study, we have predicted that all seven human coronaviruses harbor G-quadruplex sequences. Conserved G-quadruplex sequences in SARS-CoV and SARS-CoV-2 were analyzed and verified by circular dichroism (CD) spectroscopy and Thioflavin T fluorescence assay. Similar to SARS-CoV, SARS-CoV-2 encodes an nsP3 protein, which is predicted to associate with G-quadruplexes. Targeting G-quadruplex sequences in the SARS-CoV-2 genome by G-quadruplex ligands could be a new way to conquer COVID-19. Frontiers Media S.A. 2020-10-23 /pmc/articles/PMC7644843/ /pubmed/33193156 http://dx.doi.org/10.3389/fmicb.2020.567317 Text en Copyright © 2020 Cui and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cui, Haoran
Zhang, Leiliang
G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title_full G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title_fullStr G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title_full_unstemmed G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title_short G-Quadruplexes Are Present in Human Coronaviruses Including SARS-CoV-2
title_sort g-quadruplexes are present in human coronaviruses including sars-cov-2
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644843/
https://www.ncbi.nlm.nih.gov/pubmed/33193156
http://dx.doi.org/10.3389/fmicb.2020.567317
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