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Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability
The objective of this study was to ascertain the cellular and functional parameters as well as ROS related changes in sperm from bulls with varied sperm freezability phenotypes. Using principal component analysis (PCA), the variables were reduced to two principal components, of which PC1 explained 4...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644894/ https://www.ncbi.nlm.nih.gov/pubmed/33195596 http://dx.doi.org/10.3389/fvets.2020.581137 |
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author | Hitit, Mustafa Ugur, Muhammet Rasit Dinh, Thu Tran Nhat Sajeev, Dishnu Kaya, Abdullah Topper, Einko Tan, Wei Memili, Erdogan |
author_facet | Hitit, Mustafa Ugur, Muhammet Rasit Dinh, Thu Tran Nhat Sajeev, Dishnu Kaya, Abdullah Topper, Einko Tan, Wei Memili, Erdogan |
author_sort | Hitit, Mustafa |
collection | PubMed |
description | The objective of this study was to ascertain the cellular and functional parameters as well as ROS related changes in sperm from bulls with varied sperm freezability phenotypes. Using principal component analysis (PCA), the variables were reduced to two principal components, of which PC1 explained 48% of the variance, and PC2 explained 24% of the variance, and clustered animals into two distinct groups of good freezability (GF) and poor freezability (PF). In ROS associated pathophysiology, there were more dead superoxide anion positive (Dead SO+) sperm in GF bulls than those in PF (15.72 and 12.00%; P = 0.024), and that Dead SO+ and live hydrogen positive cells (live H(2)O(2)+) were positively correlated with freezability, respectively (R(2) = 0.55, P < 0.0130) and (r(s) = 0.63, P = 0.0498). Related to sperm functional integrity, sperm from PF bulls had greater dead intact acrosome (DIAC) than those from GF bulls (26.29 and 16.10%; P = 0.028) whereas sperm from GF bulls tended to have greater live intact acrosome (LIAC) than those from PF bulls (64.47 and 50.05%; P = 0.084). Sperm with dead reacted acrosome (DRAC) in PF bulls were greater compared to those in GF (19.27 and 11.48%; P = 0.007). While DIAC (R(2) = 0.56, P = 0.0124) and DRAC (R(2) = 0.57, P < 0.0111) were negatively correlated with freezability phenotype, LIAC (R(2) = 0.36, P = 0.0628) was positively correlated. Protamine deficiency (PRM) was similar between sperm from GF and PF bulls (7.20 and 0.64%; P = 0.206) and (r(s) = 0.70, P = 0.0251) was correlated with freezability. Sperm characteristics associated with cryotolerance are important for advancing both fundamental andrology and assisted reproductive technologies across mammals. |
format | Online Article Text |
id | pubmed-7644894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76448942020-11-13 Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability Hitit, Mustafa Ugur, Muhammet Rasit Dinh, Thu Tran Nhat Sajeev, Dishnu Kaya, Abdullah Topper, Einko Tan, Wei Memili, Erdogan Front Vet Sci Veterinary Science The objective of this study was to ascertain the cellular and functional parameters as well as ROS related changes in sperm from bulls with varied sperm freezability phenotypes. Using principal component analysis (PCA), the variables were reduced to two principal components, of which PC1 explained 48% of the variance, and PC2 explained 24% of the variance, and clustered animals into two distinct groups of good freezability (GF) and poor freezability (PF). In ROS associated pathophysiology, there were more dead superoxide anion positive (Dead SO+) sperm in GF bulls than those in PF (15.72 and 12.00%; P = 0.024), and that Dead SO+ and live hydrogen positive cells (live H(2)O(2)+) were positively correlated with freezability, respectively (R(2) = 0.55, P < 0.0130) and (r(s) = 0.63, P = 0.0498). Related to sperm functional integrity, sperm from PF bulls had greater dead intact acrosome (DIAC) than those from GF bulls (26.29 and 16.10%; P = 0.028) whereas sperm from GF bulls tended to have greater live intact acrosome (LIAC) than those from PF bulls (64.47 and 50.05%; P = 0.084). Sperm with dead reacted acrosome (DRAC) in PF bulls were greater compared to those in GF (19.27 and 11.48%; P = 0.007). While DIAC (R(2) = 0.56, P = 0.0124) and DRAC (R(2) = 0.57, P < 0.0111) were negatively correlated with freezability phenotype, LIAC (R(2) = 0.36, P = 0.0628) was positively correlated. Protamine deficiency (PRM) was similar between sperm from GF and PF bulls (7.20 and 0.64%; P = 0.206) and (r(s) = 0.70, P = 0.0251) was correlated with freezability. Sperm characteristics associated with cryotolerance are important for advancing both fundamental andrology and assisted reproductive technologies across mammals. Frontiers Media S.A. 2020-10-23 /pmc/articles/PMC7644894/ /pubmed/33195596 http://dx.doi.org/10.3389/fvets.2020.581137 Text en Copyright © 2020 Hitit, Ugur, Dinh, Sajeev, Kaya, Topper, Tan and Memili. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Hitit, Mustafa Ugur, Muhammet Rasit Dinh, Thu Tran Nhat Sajeev, Dishnu Kaya, Abdullah Topper, Einko Tan, Wei Memili, Erdogan Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title | Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title_full | Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title_fullStr | Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title_full_unstemmed | Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title_short | Cellular and Functional Physiopathology of Bull Sperm With Altered Sperm Freezability |
title_sort | cellular and functional physiopathology of bull sperm with altered sperm freezability |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644894/ https://www.ncbi.nlm.nih.gov/pubmed/33195596 http://dx.doi.org/10.3389/fvets.2020.581137 |
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