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Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis

Platycladi Semen, which is derived from the dried ripe seed of Platycladus orientalis, has been used for the treatment of insomnia and constipation in China for 2000 years. However, it is susceptible to fungal and aflatoxin contamination under proper humidity and temperature during storage. Although...

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Autores principales: Yu, Jingsheng, Guo, Mengyue, Jiang, Wenjun, Yang, Meihua, Pang, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644961/
https://www.ncbi.nlm.nih.gov/pubmed/33193220
http://dx.doi.org/10.3389/fmicb.2020.582679
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author Yu, Jingsheng
Guo, Mengyue
Jiang, Wenjun
Yang, Meihua
Pang, Xiaohui
author_facet Yu, Jingsheng
Guo, Mengyue
Jiang, Wenjun
Yang, Meihua
Pang, Xiaohui
author_sort Yu, Jingsheng
collection PubMed
description Platycladi Semen, which is derived from the dried ripe seed of Platycladus orientalis, has been used for the treatment of insomnia and constipation in China for 2000 years. However, it is susceptible to fungal and aflatoxin contamination under proper humidity and temperature during storage. Although aflatoxin contamination in Platycladi Semen has been reported preliminarily, few studies have been conducted on fungal infection and aflatoxin contamination simultaneously. Thus, this work aims to provide an in-depth understanding of fungal contamination in Platycladi Semen, and information on aflatoxin contamination. We focused on a comparison of the difference in fungal diversity between aflatoxin-contaminated and aflatoxin-free Platycladi Semen samples. First, aflatoxin levels in 11 Platycladi Semen samples, which were collected from local herbal markets in Shandong, Anhui, and Hebei provinces throughout China, were determined by IAC-HPLC-FLD, and positive confirmation of detected samples was performed by LC-MS/MS. The samples were divided into two groups, based on production or non-production of aflatoxin. We then used the Illumina MiSeq PE250 platform, and targeted the internal transcribed spacer two sequences to analyze the diversity and composition of the fungal microbiome, as well as to assess the presence of potential mycotoxin-producing fungi. Results showed that five samples were contaminated with aflatoxins, one of which exceeded the legal limits of Chinese Pharmacopeia Commission (2015). At the phylum level, the Ascomycota was the most dominant in all tested samples, with a relative abundance of 83.04–99.46%. Aspergillus (27.88–97.28%), Xerochrysium (0–28.49%), and Xeromyces (0–22.24%) were the three predominant genera. Furthermore, differences in fungal composition between the aflatoxin-contaminated and aflatoxin-free groups, as well as between different provinces were observed. A total of 74 species were identified, and four potential mycotoxin-producing fungi were detected in all samples, namely Aspergillus flavus, Aspergillus fumigatus, Fusarium poae, and Penicillium steckii. In conclusion, we report the great diversity of fungi associated with Platycladi Semen, highlight the risk to consumers of ingesting potent aflatoxin, and provide a reference for the safe application and quality improvement of Platycladi Semen.
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spelling pubmed-76449612020-11-13 Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis Yu, Jingsheng Guo, Mengyue Jiang, Wenjun Yang, Meihua Pang, Xiaohui Front Microbiol Microbiology Platycladi Semen, which is derived from the dried ripe seed of Platycladus orientalis, has been used for the treatment of insomnia and constipation in China for 2000 years. However, it is susceptible to fungal and aflatoxin contamination under proper humidity and temperature during storage. Although aflatoxin contamination in Platycladi Semen has been reported preliminarily, few studies have been conducted on fungal infection and aflatoxin contamination simultaneously. Thus, this work aims to provide an in-depth understanding of fungal contamination in Platycladi Semen, and information on aflatoxin contamination. We focused on a comparison of the difference in fungal diversity between aflatoxin-contaminated and aflatoxin-free Platycladi Semen samples. First, aflatoxin levels in 11 Platycladi Semen samples, which were collected from local herbal markets in Shandong, Anhui, and Hebei provinces throughout China, were determined by IAC-HPLC-FLD, and positive confirmation of detected samples was performed by LC-MS/MS. The samples were divided into two groups, based on production or non-production of aflatoxin. We then used the Illumina MiSeq PE250 platform, and targeted the internal transcribed spacer two sequences to analyze the diversity and composition of the fungal microbiome, as well as to assess the presence of potential mycotoxin-producing fungi. Results showed that five samples were contaminated with aflatoxins, one of which exceeded the legal limits of Chinese Pharmacopeia Commission (2015). At the phylum level, the Ascomycota was the most dominant in all tested samples, with a relative abundance of 83.04–99.46%. Aspergillus (27.88–97.28%), Xerochrysium (0–28.49%), and Xeromyces (0–22.24%) were the three predominant genera. Furthermore, differences in fungal composition between the aflatoxin-contaminated and aflatoxin-free groups, as well as between different provinces were observed. A total of 74 species were identified, and four potential mycotoxin-producing fungi were detected in all samples, namely Aspergillus flavus, Aspergillus fumigatus, Fusarium poae, and Penicillium steckii. In conclusion, we report the great diversity of fungi associated with Platycladi Semen, highlight the risk to consumers of ingesting potent aflatoxin, and provide a reference for the safe application and quality improvement of Platycladi Semen. Frontiers Media S.A. 2020-10-23 /pmc/articles/PMC7644961/ /pubmed/33193220 http://dx.doi.org/10.3389/fmicb.2020.582679 Text en Copyright © 2020 Yu, Guo, Jiang, Yang and Pang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yu, Jingsheng
Guo, Mengyue
Jiang, Wenjun
Yang, Meihua
Pang, Xiaohui
Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title_full Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title_fullStr Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title_full_unstemmed Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title_short Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal Herb Platycladus orientalis
title_sort assessment of the microbiome and potential aflatoxin associated with the medicinal herb platycladus orientalis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644961/
https://www.ncbi.nlm.nih.gov/pubmed/33193220
http://dx.doi.org/10.3389/fmicb.2020.582679
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