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Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons
Advances in single cell genomics and transcriptomics have shown that at tissue level there is complex cellular heterogeneity. To understand the effect of this inter-cell heterogeneity on metabolism it is essential to develop a single cell lipid profiling approach that allows the measurement of lipid...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644967/ https://www.ncbi.nlm.nih.gov/pubmed/33196026 http://dx.doi.org/10.1016/j.isci.2020.101703 |
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author | Snowden, Stuart G. Fernandes, Hugo J.R. Kent, Josh Foskolou, Stefanie Tate, Peri Field, Sarah F. Metzakopian, Emmanouil Koulman, Albert |
author_facet | Snowden, Stuart G. Fernandes, Hugo J.R. Kent, Josh Foskolou, Stefanie Tate, Peri Field, Sarah F. Metzakopian, Emmanouil Koulman, Albert |
author_sort | Snowden, Stuart G. |
collection | PubMed |
description | Advances in single cell genomics and transcriptomics have shown that at tissue level there is complex cellular heterogeneity. To understand the effect of this inter-cell heterogeneity on metabolism it is essential to develop a single cell lipid profiling approach that allows the measurement of lipids in large numbers of single cells from a population. This will provide a functional readout of cell activity and membrane structure. Using liquid extraction surface analysis coupled with high-resolution mass spectrometry we have developed a high-throughput method for untargeted single cell lipid profiling. This technological advance highlighted the importance of cellular heterogeneity in the functional metabolism of individual human dopamine neurons, suggesting that A53T alpha-synuclein (SNCA) mutant neurons have impaired membrane function. These results demonstrate that this single cell lipid profiling platform can provide robust data that will expand the frontiers in biomedical research. |
format | Online Article Text |
id | pubmed-7644967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-76449672020-11-13 Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons Snowden, Stuart G. Fernandes, Hugo J.R. Kent, Josh Foskolou, Stefanie Tate, Peri Field, Sarah F. Metzakopian, Emmanouil Koulman, Albert iScience Article Advances in single cell genomics and transcriptomics have shown that at tissue level there is complex cellular heterogeneity. To understand the effect of this inter-cell heterogeneity on metabolism it is essential to develop a single cell lipid profiling approach that allows the measurement of lipids in large numbers of single cells from a population. This will provide a functional readout of cell activity and membrane structure. Using liquid extraction surface analysis coupled with high-resolution mass spectrometry we have developed a high-throughput method for untargeted single cell lipid profiling. This technological advance highlighted the importance of cellular heterogeneity in the functional metabolism of individual human dopamine neurons, suggesting that A53T alpha-synuclein (SNCA) mutant neurons have impaired membrane function. These results demonstrate that this single cell lipid profiling platform can provide robust data that will expand the frontiers in biomedical research. Elsevier 2020-10-21 /pmc/articles/PMC7644967/ /pubmed/33196026 http://dx.doi.org/10.1016/j.isci.2020.101703 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Snowden, Stuart G. Fernandes, Hugo J.R. Kent, Josh Foskolou, Stefanie Tate, Peri Field, Sarah F. Metzakopian, Emmanouil Koulman, Albert Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title | Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title_full | Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title_fullStr | Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title_full_unstemmed | Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title_short | Development and Application of High-Throughput Single Cell Lipid Profiling: A Study of SNCA-A53T Human Dopamine Neurons |
title_sort | development and application of high-throughput single cell lipid profiling: a study of snca-a53t human dopamine neurons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644967/ https://www.ncbi.nlm.nih.gov/pubmed/33196026 http://dx.doi.org/10.1016/j.isci.2020.101703 |
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