Cargando…

Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice

Periodontitis is the second most common oral disease affecting tooth-supporting structures. The tissue damage is mainly initiated by the excessive secretion of proinflammatory cytokines by immune cells. Macrophages are a type of antigen-presenting cells that influence the adaptive immunity function....

Descripción completa

Detalles Bibliográficos
Autores principales: Miao, Yibin, He, Liuting, Qi, Xiaoyu, Lin, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645063/
https://www.ncbi.nlm.nih.gov/pubmed/33195441
http://dx.doi.org/10.3389/fmolb.2020.603817
_version_ 1783606587609317376
author Miao, Yibin
He, Liuting
Qi, Xiaoyu
Lin, Xiaoping
author_facet Miao, Yibin
He, Liuting
Qi, Xiaoyu
Lin, Xiaoping
author_sort Miao, Yibin
collection PubMed
description Periodontitis is the second most common oral disease affecting tooth-supporting structures. The tissue damage is mainly initiated by the excessive secretion of proinflammatory cytokines by immune cells. Macrophages are a type of antigen-presenting cells that influence the adaptive immunity function. We used a unique set of cytokines, i.e., a combination of IL-4, IL-13, and IL-10, to stimulate macrophages into a subset of M2 polarization cells that express much higher levels of ARG-1, CD206, and PDL-2 genes. The cells’ anti-inflammatory potential was tested with mixed-lymphocyte reaction assay, which showed that this subset of macrophages could increase IL-2 secretion and suppress IL-17, IL-6, and TNF-α secretion by splenocytes. The gram-negative bacterial species Porphyromonas gingivalis was used to initiate an inflammatory process in murine periodontal tissues. In the meantime, cell injection therapy was used to dampen the excessive immune reaction and suppress osteoclast differentiation during periodontitis. Maxilla was collected and analyzed for osteoclast formation. The results indicated that mice in the cell injection group exhibited less osteoclast activity within the periodontal ligament region than in the periodontitis group. Moreover, the injection of M2 macrophages sustained the regulatory population ratio. Therefore, the M2 macrophages induced under the stimulation of IL-4, IL-13, and IL-10 combined had tremendous immune modulation ability. Injecting these cells into local periodontal tissue could effectively alleviate the symptom of periodontitis.
format Online
Article
Text
id pubmed-7645063
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-76450632020-11-13 Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice Miao, Yibin He, Liuting Qi, Xiaoyu Lin, Xiaoping Front Mol Biosci Molecular Biosciences Periodontitis is the second most common oral disease affecting tooth-supporting structures. The tissue damage is mainly initiated by the excessive secretion of proinflammatory cytokines by immune cells. Macrophages are a type of antigen-presenting cells that influence the adaptive immunity function. We used a unique set of cytokines, i.e., a combination of IL-4, IL-13, and IL-10, to stimulate macrophages into a subset of M2 polarization cells that express much higher levels of ARG-1, CD206, and PDL-2 genes. The cells’ anti-inflammatory potential was tested with mixed-lymphocyte reaction assay, which showed that this subset of macrophages could increase IL-2 secretion and suppress IL-17, IL-6, and TNF-α secretion by splenocytes. The gram-negative bacterial species Porphyromonas gingivalis was used to initiate an inflammatory process in murine periodontal tissues. In the meantime, cell injection therapy was used to dampen the excessive immune reaction and suppress osteoclast differentiation during periodontitis. Maxilla was collected and analyzed for osteoclast formation. The results indicated that mice in the cell injection group exhibited less osteoclast activity within the periodontal ligament region than in the periodontitis group. Moreover, the injection of M2 macrophages sustained the regulatory population ratio. Therefore, the M2 macrophages induced under the stimulation of IL-4, IL-13, and IL-10 combined had tremendous immune modulation ability. Injecting these cells into local periodontal tissue could effectively alleviate the symptom of periodontitis. Frontiers Media S.A. 2020-10-23 /pmc/articles/PMC7645063/ /pubmed/33195441 http://dx.doi.org/10.3389/fmolb.2020.603817 Text en Copyright © 2020 Miao, He, Qi and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Miao, Yibin
He, Liuting
Qi, Xiaoyu
Lin, Xiaoping
Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title_full Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title_fullStr Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title_full_unstemmed Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title_short Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice
title_sort injecting immunosuppressive m2 macrophages alleviates the symptoms of periodontitis in mice
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645063/
https://www.ncbi.nlm.nih.gov/pubmed/33195441
http://dx.doi.org/10.3389/fmolb.2020.603817
work_keys_str_mv AT miaoyibin injectingimmunosuppressivem2macrophagesalleviatesthesymptomsofperiodontitisinmice
AT heliuting injectingimmunosuppressivem2macrophagesalleviatesthesymptomsofperiodontitisinmice
AT qixiaoyu injectingimmunosuppressivem2macrophagesalleviatesthesymptomsofperiodontitisinmice
AT linxiaoping injectingimmunosuppressivem2macrophagesalleviatesthesymptomsofperiodontitisinmice