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ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans
As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored h...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645159/ https://www.ncbi.nlm.nih.gov/pubmed/33192610 http://dx.doi.org/10.3389/fphys.2020.593841 |
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author | Bertolone, Lorenzo Shin, Hye K. Stefanoni, Davide Baek, Jin Hyen Gao, Yamei Morrison, Evan J. Nemkov, Travis Thomas, Tiffany Francis, Richard O. Hod, Eldad A. Zimring, James C. Yoshida, Tatsuro Karafin, Matthew Schwartz, Joseph Hudson, Krystalyn E. Spitalnik, Steven L. Buehler, Paul W. D’Alessandro, Angelo |
author_facet | Bertolone, Lorenzo Shin, Hye K. Stefanoni, Davide Baek, Jin Hyen Gao, Yamei Morrison, Evan J. Nemkov, Travis Thomas, Tiffany Francis, Richard O. Hod, Eldad A. Zimring, James C. Yoshida, Tatsuro Karafin, Matthew Schwartz, Joseph Hudson, Krystalyn E. Spitalnik, Steven L. Buehler, Paul W. D’Alessandro, Angelo |
author_sort | Bertolone, Lorenzo |
collection | PubMed |
description | As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored human (n = 21, 10 males, 11 females), olive baboon (n = 20), and rhesus macaque (n = 20) red blood cells at baseline and upon 42 days of storage under blood bank conditions. The results indicated similarities and differences across species, which ultimately resulted in a differential propensity to undergo morphological alterations and lyse as a function of the duration of refrigerated storage. Focusing on purine oxidation, carboxylic acid, fatty acid, and arginine metabolism further highlighted species-specific metabolic wiring. For example, through a combination of steady state measurements and (13)C(6)(15)N(4)-arginine tracing experiments, we report an increase in arginine catabolism into ornithine in humans, suggestive of species-specific arginase 1 activity and nitric oxide synthesis—an observation that may impact the translatability of cardiovascular disease studies carried out in non-human primates (NHPs). Finally, we correlated metabolic measurements to storage-induced morphological alterations via scanning electron microscopy and hemolysis, which were significantly lower in human red cells compared to both NHPs. |
format | Online Article Text |
id | pubmed-7645159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-76451592020-11-13 ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans Bertolone, Lorenzo Shin, Hye K. Stefanoni, Davide Baek, Jin Hyen Gao, Yamei Morrison, Evan J. Nemkov, Travis Thomas, Tiffany Francis, Richard O. Hod, Eldad A. Zimring, James C. Yoshida, Tatsuro Karafin, Matthew Schwartz, Joseph Hudson, Krystalyn E. Spitalnik, Steven L. Buehler, Paul W. D’Alessandro, Angelo Front Physiol Physiology As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored human (n = 21, 10 males, 11 females), olive baboon (n = 20), and rhesus macaque (n = 20) red blood cells at baseline and upon 42 days of storage under blood bank conditions. The results indicated similarities and differences across species, which ultimately resulted in a differential propensity to undergo morphological alterations and lyse as a function of the duration of refrigerated storage. Focusing on purine oxidation, carboxylic acid, fatty acid, and arginine metabolism further highlighted species-specific metabolic wiring. For example, through a combination of steady state measurements and (13)C(6)(15)N(4)-arginine tracing experiments, we report an increase in arginine catabolism into ornithine in humans, suggestive of species-specific arginase 1 activity and nitric oxide synthesis—an observation that may impact the translatability of cardiovascular disease studies carried out in non-human primates (NHPs). Finally, we correlated metabolic measurements to storage-induced morphological alterations via scanning electron microscopy and hemolysis, which were significantly lower in human red cells compared to both NHPs. Frontiers Media S.A. 2020-10-23 /pmc/articles/PMC7645159/ /pubmed/33192610 http://dx.doi.org/10.3389/fphys.2020.593841 Text en Copyright © 2020 Bertolone, Shin, Stefanoni, Baek, Gao, Morrison, Nemkov, Thomas, Francis, Hod, Zimring, Yoshida, Karafin, Schwartz, Hudson, Spitalnik, Buehler and D’Alessandro. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Bertolone, Lorenzo Shin, Hye K. Stefanoni, Davide Baek, Jin Hyen Gao, Yamei Morrison, Evan J. Nemkov, Travis Thomas, Tiffany Francis, Richard O. Hod, Eldad A. Zimring, James C. Yoshida, Tatsuro Karafin, Matthew Schwartz, Joseph Hudson, Krystalyn E. Spitalnik, Steven L. Buehler, Paul W. D’Alessandro, Angelo ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title | ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title_full | ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title_fullStr | ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title_full_unstemmed | ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title_short | ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans |
title_sort | zoomics: comparative metabolomics of red blood cells from old world monkeys and humans |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645159/ https://www.ncbi.nlm.nih.gov/pubmed/33192610 http://dx.doi.org/10.3389/fphys.2020.593841 |
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