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Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4

The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, and membrane or organelle dynamics. However, its filamentous properties and functions inside the mammalian cell nucleus are less well explored. We previously described transien...

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Autores principales: Krippner, Sylvia, Winkelmeier, Jannik, Knerr, Julian, Brandt, Dominique T, Virant, David, Schwan, Carsten, Endesfelder, Ulrike, Grosse, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645226/
https://www.ncbi.nlm.nih.gov/pubmed/32959960
http://dx.doi.org/10.15252/embr.202050758
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author Krippner, Sylvia
Winkelmeier, Jannik
Knerr, Julian
Brandt, Dominique T
Virant, David
Schwan, Carsten
Endesfelder, Ulrike
Grosse, Robert
author_facet Krippner, Sylvia
Winkelmeier, Jannik
Knerr, Julian
Brandt, Dominique T
Virant, David
Schwan, Carsten
Endesfelder, Ulrike
Grosse, Robert
author_sort Krippner, Sylvia
collection PubMed
description The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, and membrane or organelle dynamics. However, its filamentous properties and functions inside the mammalian cell nucleus are less well explored. We previously described transient actin assembly at mitotic exit that promotes nuclear expansion during chromatin decondensation. Here, we identify non‐muscle α‐actinin 4 (ACTN4) as a critical regulator to facilitate F‐actin reorganization and bundling during postmitotic nuclear expansion. ACTN4 binds to nuclear actin filament structures, and ACTN4 clusters associate with nuclear F‐actin in a highly dynamic fashion. ACTN4 but not ACTN1 is required for proper postmitotic nuclear volume expansion, mediated by its actin‐binding domain. Using super‐resolution imaging to quantify actin filament numbers and widths in individual nuclei, we find that ACTN4 is necessary for postmitotic nuclear actin reorganization and actin filament bundling. Our findings uncover a nuclear cytoskeletal function for ACTN4 to control nuclear size and chromatin organization during mitotic cell division.
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spelling pubmed-76452262020-11-13 Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4 Krippner, Sylvia Winkelmeier, Jannik Knerr, Julian Brandt, Dominique T Virant, David Schwan, Carsten Endesfelder, Ulrike Grosse, Robert EMBO Rep Reports The actin cytoskeleton operates in a multitude of cellular processes including cell shape and migration, mechanoregulation, and membrane or organelle dynamics. However, its filamentous properties and functions inside the mammalian cell nucleus are less well explored. We previously described transient actin assembly at mitotic exit that promotes nuclear expansion during chromatin decondensation. Here, we identify non‐muscle α‐actinin 4 (ACTN4) as a critical regulator to facilitate F‐actin reorganization and bundling during postmitotic nuclear expansion. ACTN4 binds to nuclear actin filament structures, and ACTN4 clusters associate with nuclear F‐actin in a highly dynamic fashion. ACTN4 but not ACTN1 is required for proper postmitotic nuclear volume expansion, mediated by its actin‐binding domain. Using super‐resolution imaging to quantify actin filament numbers and widths in individual nuclei, we find that ACTN4 is necessary for postmitotic nuclear actin reorganization and actin filament bundling. Our findings uncover a nuclear cytoskeletal function for ACTN4 to control nuclear size and chromatin organization during mitotic cell division. John Wiley and Sons Inc. 2020-09-22 2020-11-05 /pmc/articles/PMC7645226/ /pubmed/32959960 http://dx.doi.org/10.15252/embr.202050758 Text en © 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Reports
Krippner, Sylvia
Winkelmeier, Jannik
Knerr, Julian
Brandt, Dominique T
Virant, David
Schwan, Carsten
Endesfelder, Ulrike
Grosse, Robert
Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title_full Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title_fullStr Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title_full_unstemmed Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title_short Postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
title_sort postmitotic expansion of cell nuclei requires nuclear actin filament bundling by α‐actinin 4
topic Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645226/
https://www.ncbi.nlm.nih.gov/pubmed/32959960
http://dx.doi.org/10.15252/embr.202050758
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