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Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis
Canine diabetes mellitus (DM) affects 0.6% of the canine population and yet, its etiology is poorly understood. Most affected dogs are diagnosed as adults and are insulin-dependent. We compared pan-leukocyte and sympathetic innervation markers in pancreatic islets of adult dogs with spontaneous DM (...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645777/ https://www.ncbi.nlm.nih.gov/pubmed/33154408 http://dx.doi.org/10.1038/s41598-020-76091-5 |
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author | Gilor, Chen Pires, Jully Greathouse, Rachel Horn, Rebecca Huising, Mark O. Marks, Stanley L. Murphy, Brian Kol, Amir |
author_facet | Gilor, Chen Pires, Jully Greathouse, Rachel Horn, Rebecca Huising, Mark O. Marks, Stanley L. Murphy, Brian Kol, Amir |
author_sort | Gilor, Chen |
collection | PubMed |
description | Canine diabetes mellitus (DM) affects 0.6% of the canine population and yet, its etiology is poorly understood. Most affected dogs are diagnosed as adults and are insulin-dependent. We compared pan-leukocyte and sympathetic innervation markers in pancreatic islets of adult dogs with spontaneous DM (sDM), spontaneous pancreatitis (sPanc), both (sDMPanc), toxin-induced DM (iDM) and controls. We found evidence of decreased islet sympathetic innervation but no significant infiltration of islets with leukocytes in all disease groups. We show that loss of sympathetic innervation is ongoing in canine DM and does not necessarily precede it. We further found selective loss of islet-associated beta cells in dogs with sDM and sDMPanc, suggesting that collateral damage from inflammation in the exocrine pancreas is not a likely cause of DM in these dogs. The cause of this selective loss of beta cells needs to be further elucidated but overall, our findings are not supportive of an autoimmune process as a cause of sDM in adult dogs. The loss of sympathetic innervation in sPanc in dogs that do not suffer from DM links the disease in the exocrine pancreas to a pathological process in the endocrine pancreas, suggesting pancreatitis might be a potential precursor to DM. |
format | Online Article Text |
id | pubmed-7645777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-76457772020-11-06 Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis Gilor, Chen Pires, Jully Greathouse, Rachel Horn, Rebecca Huising, Mark O. Marks, Stanley L. Murphy, Brian Kol, Amir Sci Rep Article Canine diabetes mellitus (DM) affects 0.6% of the canine population and yet, its etiology is poorly understood. Most affected dogs are diagnosed as adults and are insulin-dependent. We compared pan-leukocyte and sympathetic innervation markers in pancreatic islets of adult dogs with spontaneous DM (sDM), spontaneous pancreatitis (sPanc), both (sDMPanc), toxin-induced DM (iDM) and controls. We found evidence of decreased islet sympathetic innervation but no significant infiltration of islets with leukocytes in all disease groups. We show that loss of sympathetic innervation is ongoing in canine DM and does not necessarily precede it. We further found selective loss of islet-associated beta cells in dogs with sDM and sDMPanc, suggesting that collateral damage from inflammation in the exocrine pancreas is not a likely cause of DM in these dogs. The cause of this selective loss of beta cells needs to be further elucidated but overall, our findings are not supportive of an autoimmune process as a cause of sDM in adult dogs. The loss of sympathetic innervation in sPanc in dogs that do not suffer from DM links the disease in the exocrine pancreas to a pathological process in the endocrine pancreas, suggesting pancreatitis might be a potential precursor to DM. Nature Publishing Group UK 2020-11-05 /pmc/articles/PMC7645777/ /pubmed/33154408 http://dx.doi.org/10.1038/s41598-020-76091-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gilor, Chen Pires, Jully Greathouse, Rachel Horn, Rebecca Huising, Mark O. Marks, Stanley L. Murphy, Brian Kol, Amir Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title | Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title_full | Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title_fullStr | Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title_full_unstemmed | Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title_short | Loss of sympathetic innervation to islets of Langerhans in canine diabetes and pancreatitis is not associated with insulitis |
title_sort | loss of sympathetic innervation to islets of langerhans in canine diabetes and pancreatitis is not associated with insulitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7645777/ https://www.ncbi.nlm.nih.gov/pubmed/33154408 http://dx.doi.org/10.1038/s41598-020-76091-5 |
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