Cargando…
In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity
BACKGROUND: Human cardiac stem cells expressing the W8B2 marker (W8B2(+) CSCs) were recently identified and proposed as a new model of multipotent CSCs capable of differentiating into smooth muscle cells, endothelial cells and immature myocytes. Nevertheless, no characterization of ion channel or ca...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646077/ https://www.ncbi.nlm.nih.gov/pubmed/33292162 http://dx.doi.org/10.1186/s11658-020-00242-9 |
_version_ | 1783606727760936960 |
---|---|
author | Ayad, Oualid Al Sayed, Zeina R. Sebille, Stéphane Magaud, Christophe Chapotte-Baldacci, Charles-Albert Jayle, Christophe Faivre, Jean-François Gaborit, Nathalie Chatelier, Aurélien Bois, Patrick |
author_facet | Ayad, Oualid Al Sayed, Zeina R. Sebille, Stéphane Magaud, Christophe Chapotte-Baldacci, Charles-Albert Jayle, Christophe Faivre, Jean-François Gaborit, Nathalie Chatelier, Aurélien Bois, Patrick |
author_sort | Ayad, Oualid |
collection | PubMed |
description | BACKGROUND: Human cardiac stem cells expressing the W8B2 marker (W8B2(+) CSCs) were recently identified and proposed as a new model of multipotent CSCs capable of differentiating into smooth muscle cells, endothelial cells and immature myocytes. Nevertheless, no characterization of ion channel or calcium activity during the differentiation of these stem cells has been reported. METHODS: The objectives of this study were thus to analyze (using the TaqMan Low-Density Array technique) the gene profile of W8B2(+) CSCs pertaining to the regulation of ion channels, transporters and other players involved in the calcium homeostasis of these cells. We also analyzed spontaneous calcium activity (via the GCaMP calcium probe) during the in vitro differentiation of W8B2(+) CSCs into cardiac myocytes. RESULTS: Our results show an entirely different electrophysiological genomic profile between W8B2(+) CSCs before and after differentiation. Some specific nodal genes, such as Tbx3, HCN, ICaT, L, KV, and NCX, are overexpressed after this differentiation. In addition, we reveal spontaneous calcium activity or a calcium clock whose kinetics change during the differentiation process. A pharmacological study carried out on differentiated W8B2(+) CSCs showed that the NCX exchanger and IP3 stores play a fundamental role in the generation of these calcium oscillations. CONCLUSIONS: Taken together, the present results provide important information on ion channel expression and intrinsic calcium dynamics during the differentiation process of stem cells expressing the W8B2 marker. |
format | Online Article Text |
id | pubmed-7646077 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76460772020-11-06 In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity Ayad, Oualid Al Sayed, Zeina R. Sebille, Stéphane Magaud, Christophe Chapotte-Baldacci, Charles-Albert Jayle, Christophe Faivre, Jean-François Gaborit, Nathalie Chatelier, Aurélien Bois, Patrick Cell Mol Biol Lett Research Letter BACKGROUND: Human cardiac stem cells expressing the W8B2 marker (W8B2(+) CSCs) were recently identified and proposed as a new model of multipotent CSCs capable of differentiating into smooth muscle cells, endothelial cells and immature myocytes. Nevertheless, no characterization of ion channel or calcium activity during the differentiation of these stem cells has been reported. METHODS: The objectives of this study were thus to analyze (using the TaqMan Low-Density Array technique) the gene profile of W8B2(+) CSCs pertaining to the regulation of ion channels, transporters and other players involved in the calcium homeostasis of these cells. We also analyzed spontaneous calcium activity (via the GCaMP calcium probe) during the in vitro differentiation of W8B2(+) CSCs into cardiac myocytes. RESULTS: Our results show an entirely different electrophysiological genomic profile between W8B2(+) CSCs before and after differentiation. Some specific nodal genes, such as Tbx3, HCN, ICaT, L, KV, and NCX, are overexpressed after this differentiation. In addition, we reveal spontaneous calcium activity or a calcium clock whose kinetics change during the differentiation process. A pharmacological study carried out on differentiated W8B2(+) CSCs showed that the NCX exchanger and IP3 stores play a fundamental role in the generation of these calcium oscillations. CONCLUSIONS: Taken together, the present results provide important information on ion channel expression and intrinsic calcium dynamics during the differentiation process of stem cells expressing the W8B2 marker. BioMed Central 2020-11-05 /pmc/articles/PMC7646077/ /pubmed/33292162 http://dx.doi.org/10.1186/s11658-020-00242-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Letter Ayad, Oualid Al Sayed, Zeina R. Sebille, Stéphane Magaud, Christophe Chapotte-Baldacci, Charles-Albert Jayle, Christophe Faivre, Jean-François Gaborit, Nathalie Chatelier, Aurélien Bois, Patrick In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title | In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title_full | In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title_fullStr | In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title_full_unstemmed | In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title_short | In vitro differentiation of W8B2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
title_sort | in vitro differentiation of w8b2(+) human cardiac stem cells: gene expression of ionic channels and spontaneous calcium activity |
topic | Research Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646077/ https://www.ncbi.nlm.nih.gov/pubmed/33292162 http://dx.doi.org/10.1186/s11658-020-00242-9 |
work_keys_str_mv | AT ayadoualid invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT alsayedzeinar invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT sebillestephane invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT magaudchristophe invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT chapottebaldaccicharlesalbert invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT jaylechristophe invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT faivrejeanfrancois invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT gaboritnathalie invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT chatelieraurelien invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity AT boispatrick invitrodifferentiationofw8b2humancardiacstemcellsgeneexpressionofionicchannelsandspontaneouscalciumactivity |