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A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis

Occludin is a structural protein of tight junctions (TJ) in the blood–testis barrier (BTB). A 22-amino-acid peptide (22AA) in the second extracellular loop can reversibly regulate TJ, but its regulatory mechanism is unknown. In this study, a 22AA-induced TJ destruction animal model was constructed t...

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Autores principales: Zhu, Maoying, Lu, Juan, Shen, Jianyun, Fei, Lumin, Chen, Deyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646304/
https://www.ncbi.nlm.nih.gov/pubmed/33194394
http://dx.doi.org/10.7717/peerj.10147
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author Zhu, Maoying
Lu, Juan
Shen, Jianyun
Fei, Lumin
Chen, Deyu
author_facet Zhu, Maoying
Lu, Juan
Shen, Jianyun
Fei, Lumin
Chen, Deyu
author_sort Zhu, Maoying
collection PubMed
description Occludin is a structural protein of tight junctions (TJ) in the blood–testis barrier (BTB). A 22-amino-acid peptide (22AA) in the second extracellular loop can reversibly regulate TJ, but its regulatory mechanism is unknown. In this study, a 22AA-induced TJ destruction animal model was constructed to investigate the effect of 22AA on Sertoli cells (SCs) and spermatid counts and cell apoptosis at different time points using a multiplex immunofluorescence technique. The effect of 22AA on the location and distribution of occludin was analyzed via dual confocal fluorescence microscope. Western blotting was used to analyze dynamic changes in occludin expression. Real-time RT-PCR was used to analyze miR-122-5p expression changes. Sperm density counts and mating methods were used to analyze the effect of 22AA on fertility in mice. The results showed that 22AA promoted SC and spermatid apoptosis, downregulated occludin, upregulated miR-122-5p, and decreased sperm density and litter size before 27 days (27D). After 27D, the expression of occludin increased again, miR-122-5p expression decreased again, both sperm density and litter size returned to normal, apoptosis stopped, and spermatogenesis began to recover. Therefore, it can be concluded that 22AA can destroy TJ by downregulating occludin and inducing cell apoptosis. After 27D, TJ and spermatogenesis functions return to normal.
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spelling pubmed-76463042020-11-12 A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis Zhu, Maoying Lu, Juan Shen, Jianyun Fei, Lumin Chen, Deyu PeerJ Biochemistry Occludin is a structural protein of tight junctions (TJ) in the blood–testis barrier (BTB). A 22-amino-acid peptide (22AA) in the second extracellular loop can reversibly regulate TJ, but its regulatory mechanism is unknown. In this study, a 22AA-induced TJ destruction animal model was constructed to investigate the effect of 22AA on Sertoli cells (SCs) and spermatid counts and cell apoptosis at different time points using a multiplex immunofluorescence technique. The effect of 22AA on the location and distribution of occludin was analyzed via dual confocal fluorescence microscope. Western blotting was used to analyze dynamic changes in occludin expression. Real-time RT-PCR was used to analyze miR-122-5p expression changes. Sperm density counts and mating methods were used to analyze the effect of 22AA on fertility in mice. The results showed that 22AA promoted SC and spermatid apoptosis, downregulated occludin, upregulated miR-122-5p, and decreased sperm density and litter size before 27 days (27D). After 27D, the expression of occludin increased again, miR-122-5p expression decreased again, both sperm density and litter size returned to normal, apoptosis stopped, and spermatogenesis began to recover. Therefore, it can be concluded that 22AA can destroy TJ by downregulating occludin and inducing cell apoptosis. After 27D, TJ and spermatogenesis functions return to normal. PeerJ Inc. 2020-11-03 /pmc/articles/PMC7646304/ /pubmed/33194394 http://dx.doi.org/10.7717/peerj.10147 Text en ©2020 Zhu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Zhu, Maoying
Lu, Juan
Shen, Jianyun
Fei, Lumin
Chen, Deyu
A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title_full A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title_fullStr A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title_full_unstemmed A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title_short A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
title_sort 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646304/
https://www.ncbi.nlm.nih.gov/pubmed/33194394
http://dx.doi.org/10.7717/peerj.10147
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