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Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China

BACKGROUND: A carbapenem-resistant Escherichia coli (sequence type 5415) strain was isolated from a male patient through routine surveillance in 2018 in Guangzhou, China. MATERIALS AND METHODS: Bacteria were isolated from a sputum culture and identified by using the Vitek 2 compact system. The bla(N...

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Autores principales: Yin, Dongdong, Lin, Yanfeng, Li, Zhonghong, Ma, Hui, Lu, Lanfen, Wang, Kaiying, Yang, Lang, Du, Xinying, Li, Peng, Qi, Kezong, Song, Hongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646509/
https://www.ncbi.nlm.nih.gov/pubmed/33173318
http://dx.doi.org/10.2147/IDR.S277997
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author Yin, Dongdong
Lin, Yanfeng
Li, Zhonghong
Ma, Hui
Lu, Lanfen
Wang, Kaiying
Yang, Lang
Du, Xinying
Li, Peng
Qi, Kezong
Song, Hongbin
author_facet Yin, Dongdong
Lin, Yanfeng
Li, Zhonghong
Ma, Hui
Lu, Lanfen
Wang, Kaiying
Yang, Lang
Du, Xinying
Li, Peng
Qi, Kezong
Song, Hongbin
author_sort Yin, Dongdong
collection PubMed
description BACKGROUND: A carbapenem-resistant Escherichia coli (sequence type 5415) strain was isolated from a male patient through routine surveillance in 2018 in Guangzhou, China. MATERIALS AND METHODS: Bacteria were isolated from a sputum culture and identified by using the Vitek 2 compact system. The bla(NDM-5) gene was amplified and confirmed by sequencing. Antimicrobial susceptibility testing was determined by a Vitek 2 compact system. The bla(NDM-5) gene was located by Southern blotting. Whole-genome sequencing was carried out using both Illumina MiSeq and Oxford Nanopore MinION. RESULTS: S1-PFGE and Southern blotting showed that the bla(NDM-5) gene was located on a novel 66-kb IncFII [F2:A-:B-] plasmid. Conjugation assays revealed that the bla(NDM-5)-bearing plasmid was self-transferrable. Genomic sequencing and comparative analysis suggested that plasmid p2947-NDM5 likely originated from a combination of an IncFII-type backbone and the bla(NDM-5) flanking genetic elements. CONCLUSION: This is the first report of an ST5414 E. coli strain expressing an NDM-5 β-lactamase. This study highlights the genetic complexity of bla(NDM-5) carrying plasmids and the urgent need for continuous active monitoring.
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spelling pubmed-76465092020-11-09 Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China Yin, Dongdong Lin, Yanfeng Li, Zhonghong Ma, Hui Lu, Lanfen Wang, Kaiying Yang, Lang Du, Xinying Li, Peng Qi, Kezong Song, Hongbin Infect Drug Resist Original Research BACKGROUND: A carbapenem-resistant Escherichia coli (sequence type 5415) strain was isolated from a male patient through routine surveillance in 2018 in Guangzhou, China. MATERIALS AND METHODS: Bacteria were isolated from a sputum culture and identified by using the Vitek 2 compact system. The bla(NDM-5) gene was amplified and confirmed by sequencing. Antimicrobial susceptibility testing was determined by a Vitek 2 compact system. The bla(NDM-5) gene was located by Southern blotting. Whole-genome sequencing was carried out using both Illumina MiSeq and Oxford Nanopore MinION. RESULTS: S1-PFGE and Southern blotting showed that the bla(NDM-5) gene was located on a novel 66-kb IncFII [F2:A-:B-] plasmid. Conjugation assays revealed that the bla(NDM-5)-bearing plasmid was self-transferrable. Genomic sequencing and comparative analysis suggested that plasmid p2947-NDM5 likely originated from a combination of an IncFII-type backbone and the bla(NDM-5) flanking genetic elements. CONCLUSION: This is the first report of an ST5414 E. coli strain expressing an NDM-5 β-lactamase. This study highlights the genetic complexity of bla(NDM-5) carrying plasmids and the urgent need for continuous active monitoring. Dove 2020-11-02 /pmc/articles/PMC7646509/ /pubmed/33173318 http://dx.doi.org/10.2147/IDR.S277997 Text en © 2020 Yin et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yin, Dongdong
Lin, Yanfeng
Li, Zhonghong
Ma, Hui
Lu, Lanfen
Wang, Kaiying
Yang, Lang
Du, Xinying
Li, Peng
Qi, Kezong
Song, Hongbin
Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title_full Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title_fullStr Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title_full_unstemmed Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title_short Characterization of a Novel NDM-5-Harboring Plasmid from a Carbapenem-Resistant Escherichia coli Isolate from China
title_sort characterization of a novel ndm-5-harboring plasmid from a carbapenem-resistant escherichia coli isolate from china
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646509/
https://www.ncbi.nlm.nih.gov/pubmed/33173318
http://dx.doi.org/10.2147/IDR.S277997
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