Cargando…

Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China

The Liu’s skink, Plestiodon liui, is endemic to China. In this study, a nearly complete mitochondrial genome (mitogenome) (17,643 bp in length) of P. liui from Junzhang Mountain, Wuxi, Jiangsu province, China, was determined by next-generation sequencing. Similar to the typical mtDNA of vertebrates,...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Na, Cai, Bo, Chen, Minli, Guo, Xianguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646586/
https://www.ncbi.nlm.nih.gov/pubmed/33367047
http://dx.doi.org/10.1080/23802359.2020.1832594
_version_ 1783606820287283200
author Wu, Na
Cai, Bo
Chen, Minli
Guo, Xianguang
author_facet Wu, Na
Cai, Bo
Chen, Minli
Guo, Xianguang
author_sort Wu, Na
collection PubMed
description The Liu’s skink, Plestiodon liui, is endemic to China. In this study, a nearly complete mitochondrial genome (mitogenome) (17,643 bp in length) of P. liui from Junzhang Mountain, Wuxi, Jiangsu province, China, was determined by next-generation sequencing. Similar to the typical mtDNA of vertebrates, it contained two ribosomal RNA genes, 13 protein-coding genes (PCGs), 22 transfer RNA genes, and one control region (CR). With exception to the CR, all of the 37 genes were completely recovered. The PCGs were used to perform Bayesian phylogenetic analyses together with other scincid lizards with mitogenome data in GenBank. The resulting phylogenetic tree supported the monophyly of the P. capito group, and suggested that P. liui is the sister taxon to P. capito plus P. tunganus. The mitogenome of P. liui will provide fundamental data for the exploration of the mitogenome evolution in skinks.
format Online
Article
Text
id pubmed-7646586
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-76465862020-12-22 Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China Wu, Na Cai, Bo Chen, Minli Guo, Xianguang Mitochondrial DNA B Resour Mitogenome Announcement The Liu’s skink, Plestiodon liui, is endemic to China. In this study, a nearly complete mitochondrial genome (mitogenome) (17,643 bp in length) of P. liui from Junzhang Mountain, Wuxi, Jiangsu province, China, was determined by next-generation sequencing. Similar to the typical mtDNA of vertebrates, it contained two ribosomal RNA genes, 13 protein-coding genes (PCGs), 22 transfer RNA genes, and one control region (CR). With exception to the CR, all of the 37 genes were completely recovered. The PCGs were used to perform Bayesian phylogenetic analyses together with other scincid lizards with mitogenome data in GenBank. The resulting phylogenetic tree supported the monophyly of the P. capito group, and suggested that P. liui is the sister taxon to P. capito plus P. tunganus. The mitogenome of P. liui will provide fundamental data for the exploration of the mitogenome evolution in skinks. Taylor & Francis 2020-11-03 /pmc/articles/PMC7646586/ /pubmed/33367047 http://dx.doi.org/10.1080/23802359.2020.1832594 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mitogenome Announcement
Wu, Na
Cai, Bo
Chen, Minli
Guo, Xianguang
Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title_full Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title_fullStr Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title_full_unstemmed Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title_short Next-generation sequencing yields a nearly complete mitochondrial genome of Plestiodon liui (Reptilia, Squamata, Scincidae) endemic to China
title_sort next-generation sequencing yields a nearly complete mitochondrial genome of plestiodon liui (reptilia, squamata, scincidae) endemic to china
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646586/
https://www.ncbi.nlm.nih.gov/pubmed/33367047
http://dx.doi.org/10.1080/23802359.2020.1832594
work_keys_str_mv AT wuna nextgenerationsequencingyieldsanearlycompletemitochondrialgenomeofplestiodonliuireptiliasquamatascincidaeendemictochina
AT caibo nextgenerationsequencingyieldsanearlycompletemitochondrialgenomeofplestiodonliuireptiliasquamatascincidaeendemictochina
AT chenminli nextgenerationsequencingyieldsanearlycompletemitochondrialgenomeofplestiodonliuireptiliasquamatascincidaeendemictochina
AT guoxianguang nextgenerationsequencingyieldsanearlycompletemitochondrialgenomeofplestiodonliuireptiliasquamatascincidaeendemictochina