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Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts

The exact mechanisms underlying hypertrophic scarring is yet to be fully understood. However, excessive collagen deposition by fibroblasts has been demonstrated to result in hypertrophic scar formation, and collagen synthesis in dermal fibroblasts is regulated by the transforming growth factor-β1/Sm...

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Autores principales: Fan, Chen, El Andaloussi, Samir, Lehto, Taavi, Kong, Kiat Whye, Seow, Yiqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646840/
https://www.ncbi.nlm.nih.gov/pubmed/33173952
http://dx.doi.org/10.3892/mmr.2020.11549
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author Fan, Chen
El Andaloussi, Samir
Lehto, Taavi
Kong, Kiat Whye
Seow, Yiqi
author_facet Fan, Chen
El Andaloussi, Samir
Lehto, Taavi
Kong, Kiat Whye
Seow, Yiqi
author_sort Fan, Chen
collection PubMed
description The exact mechanisms underlying hypertrophic scarring is yet to be fully understood. However, excessive collagen deposition by fibroblasts has been demonstrated to result in hypertrophic scar formation, and collagen synthesis in dermal fibroblasts is regulated by the transforming growth factor-β1/Smad signaling pathway. In view of this, a Smad-binding decoy was designed and its effects on hypertrophic scar-derived human skin fibroblasts was evaluated. The results of the present study revealed that the Smad decoy attenuates the total amount of collagen, collagen I and Smad2/3 expression in scar fibroblasts. Data from RNA sequencing indicated that the Smad decoy induced more than 4-fold change in 178 genes, primarily associated with to the extracellular matrix, compared with the untreated control. In addition, results from quantitative real-time polymerase chain reaction further confirmed that the Smad decoy significantly attenuated the expression of extracellular matrix-related genes, including COL1A1, COL1A2 and COL3A1. Furthermore, the Smad decoy reduced transforming growth factor-β1-induced collagen deposition in scar fibroblasts. Data generated from the present study provide evidence supporting the use of the Smad decoy as a potential hypertrophic scar treatment.
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spelling pubmed-76468402020-11-10 Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts Fan, Chen El Andaloussi, Samir Lehto, Taavi Kong, Kiat Whye Seow, Yiqi Mol Med Rep Articles The exact mechanisms underlying hypertrophic scarring is yet to be fully understood. However, excessive collagen deposition by fibroblasts has been demonstrated to result in hypertrophic scar formation, and collagen synthesis in dermal fibroblasts is regulated by the transforming growth factor-β1/Smad signaling pathway. In view of this, a Smad-binding decoy was designed and its effects on hypertrophic scar-derived human skin fibroblasts was evaluated. The results of the present study revealed that the Smad decoy attenuates the total amount of collagen, collagen I and Smad2/3 expression in scar fibroblasts. Data from RNA sequencing indicated that the Smad decoy induced more than 4-fold change in 178 genes, primarily associated with to the extracellular matrix, compared with the untreated control. In addition, results from quantitative real-time polymerase chain reaction further confirmed that the Smad decoy significantly attenuated the expression of extracellular matrix-related genes, including COL1A1, COL1A2 and COL3A1. Furthermore, the Smad decoy reduced transforming growth factor-β1-induced collagen deposition in scar fibroblasts. Data generated from the present study provide evidence supporting the use of the Smad decoy as a potential hypertrophic scar treatment. D.A. Spandidos 2020-12 2020-09-29 /pmc/articles/PMC7646840/ /pubmed/33173952 http://dx.doi.org/10.3892/mmr.2020.11549 Text en Copyright: © Fan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Fan, Chen
El Andaloussi, Samir
Lehto, Taavi
Kong, Kiat Whye
Seow, Yiqi
Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title_full Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title_fullStr Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title_full_unstemmed Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title_short Smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
title_sort smad-binding decoy reduces extracellular matrix expression in human hypertrophic scar fibroblasts
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646840/
https://www.ncbi.nlm.nih.gov/pubmed/33173952
http://dx.doi.org/10.3892/mmr.2020.11549
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