Cargando…

Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia

Early-onset preeclampsia (EOPE) is a serious threat to maternal and foetal health. The present study aimed to identify potential biomarkers and targets for the treatment of EOPE. Expression profiles of placenta from patients with EOPE and healthy controls (GSE103542, GSE74341 and GSE44711) were down...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Hao, Xue, Lu, Lv, Yan, Yu, Xiang, Zheng, Yiwei, Miao, Zhijing, Ding, Hongjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646902/
https://www.ncbi.nlm.nih.gov/pubmed/33173953
http://dx.doi.org/10.3892/mmr.2020.11551
_version_ 1783606863274704896
author Zhang, Hao
Xue, Lu
Lv, Yan
Yu, Xiang
Zheng, Yiwei
Miao, Zhijing
Ding, Hongjuan
author_facet Zhang, Hao
Xue, Lu
Lv, Yan
Yu, Xiang
Zheng, Yiwei
Miao, Zhijing
Ding, Hongjuan
author_sort Zhang, Hao
collection PubMed
description Early-onset preeclampsia (EOPE) is a serious threat to maternal and foetal health. The present study aimed to identify potential biomarkers and targets for the treatment of EOPE. Expression profiles of placenta from patients with EOPE and healthy controls (GSE103542, GSE74341 and GSE44711) were downloaded from the Gene Expression Omnibus database. Integrated analysis revealed 246 genes and 28 microRNAs (miRNAs) that were differentially expressed between patients with EOPE and healthy controls. Differentially expressed genes (DEGs) were primarily enriched in ‘biological processes’, such as ‘cell adhesion’, ‘female pregnancy’, ‘extracellular matrix organization’ and ‘response to hypoxia’. Significant pathways associated with DEGs primarily included ‘focal adhesion’, ‘ECM-receptor interaction’, ‘PI3K-Akt signaling’ and ‘ovarian steroidogenesis’. A Protein-Protein Interaction network of DEGs was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins online database, and epidermal growth factor receptor, collagen α-1(I) chain, secreted phosphoprotein 1, leptin (LEP), collagen α-2(I) chain (COL1A2), plasminogen activator inhibitor 1 (SERPINE1), Thy-1 membrane glycoprotein, bone morphogenetic protein 4, vascular cell adhesion protein 1 and matrix metallopeptidase 1 were identified as hub genes. The alterations of hsa-miR-937, hsa-miR-148b*, hsa-miR-3907, hsa-miR-367*, COL1A2, LEP and SERPINE1 in placenta were validated using our local samples. Our research showed that the expression of hsa-miR-937, hsa-miR-1486*, hsa-miR-3907, hsa-miR-367* and hub genes in the placenta were closely associated with the pathophysiology of EOPE. hsa-miR-937, hsa-miR-1486*, hsa-miR-3907, hsa-miR-367* and hub genes could serve as biomarkers for diagnosis and as potential targets for the treatment of EOPE.
format Online
Article
Text
id pubmed-7646902
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-76469022020-11-13 Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia Zhang, Hao Xue, Lu Lv, Yan Yu, Xiang Zheng, Yiwei Miao, Zhijing Ding, Hongjuan Mol Med Rep Articles Early-onset preeclampsia (EOPE) is a serious threat to maternal and foetal health. The present study aimed to identify potential biomarkers and targets for the treatment of EOPE. Expression profiles of placenta from patients with EOPE and healthy controls (GSE103542, GSE74341 and GSE44711) were downloaded from the Gene Expression Omnibus database. Integrated analysis revealed 246 genes and 28 microRNAs (miRNAs) that were differentially expressed between patients with EOPE and healthy controls. Differentially expressed genes (DEGs) were primarily enriched in ‘biological processes’, such as ‘cell adhesion’, ‘female pregnancy’, ‘extracellular matrix organization’ and ‘response to hypoxia’. Significant pathways associated with DEGs primarily included ‘focal adhesion’, ‘ECM-receptor interaction’, ‘PI3K-Akt signaling’ and ‘ovarian steroidogenesis’. A Protein-Protein Interaction network of DEGs was constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins online database, and epidermal growth factor receptor, collagen α-1(I) chain, secreted phosphoprotein 1, leptin (LEP), collagen α-2(I) chain (COL1A2), plasminogen activator inhibitor 1 (SERPINE1), Thy-1 membrane glycoprotein, bone morphogenetic protein 4, vascular cell adhesion protein 1 and matrix metallopeptidase 1 were identified as hub genes. The alterations of hsa-miR-937, hsa-miR-148b*, hsa-miR-3907, hsa-miR-367*, COL1A2, LEP and SERPINE1 in placenta were validated using our local samples. Our research showed that the expression of hsa-miR-937, hsa-miR-1486*, hsa-miR-3907, hsa-miR-367* and hub genes in the placenta were closely associated with the pathophysiology of EOPE. hsa-miR-937, hsa-miR-1486*, hsa-miR-3907, hsa-miR-367* and hub genes could serve as biomarkers for diagnosis and as potential targets for the treatment of EOPE. D.A. Spandidos 2020-12 2020-09-30 /pmc/articles/PMC7646902/ /pubmed/33173953 http://dx.doi.org/10.3892/mmr.2020.11551 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Hao
Xue, Lu
Lv, Yan
Yu, Xiang
Zheng, Yiwei
Miao, Zhijing
Ding, Hongjuan
Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title_full Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title_fullStr Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title_full_unstemmed Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title_short Integrated microarray analysis of key genes and a miRNA-mRNA regulatory network of early-onset preeclampsia
title_sort integrated microarray analysis of key genes and a mirna-mrna regulatory network of early-onset preeclampsia
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7646902/
https://www.ncbi.nlm.nih.gov/pubmed/33173953
http://dx.doi.org/10.3892/mmr.2020.11551
work_keys_str_mv AT zhanghao integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT xuelu integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT lvyan integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT yuxiang integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT zhengyiwei integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT miaozhijing integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia
AT dinghongjuan integratedmicroarrayanalysisofkeygenesandamirnamrnaregulatorynetworkofearlyonsetpreeclampsia