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Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod sp...

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Autores principales: Gittens, Rolando A., Almanza, Alejandro, Bennett, Kelly L., Mejía, Luis C., Sanchez-Galan, Javier E., Merchan, Fernando, Kern, Jonathan, Miller, Matthew J., Esser, Helen J., Hwang, Robert, Dong, May, De León, Luis F., Álvarez, Eric, Loaiza, Jose R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647123/
https://www.ncbi.nlm.nih.gov/pubmed/33108372
http://dx.doi.org/10.1371/journal.pntd.0008849
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author Gittens, Rolando A.
Almanza, Alejandro
Bennett, Kelly L.
Mejía, Luis C.
Sanchez-Galan, Javier E.
Merchan, Fernando
Kern, Jonathan
Miller, Matthew J.
Esser, Helen J.
Hwang, Robert
Dong, May
De León, Luis F.
Álvarez, Eric
Loaiza, Jose R.
author_facet Gittens, Rolando A.
Almanza, Alejandro
Bennett, Kelly L.
Mejía, Luis C.
Sanchez-Galan, Javier E.
Merchan, Fernando
Kern, Jonathan
Miller, Matthew J.
Esser, Helen J.
Hwang, Robert
Dong, May
De León, Luis F.
Álvarez, Eric
Loaiza, Jose R.
author_sort Gittens, Rolando A.
collection PubMed
description Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens.
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spelling pubmed-76471232020-11-16 Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library Gittens, Rolando A. Almanza, Alejandro Bennett, Kelly L. Mejía, Luis C. Sanchez-Galan, Javier E. Merchan, Fernando Kern, Jonathan Miller, Matthew J. Esser, Helen J. Hwang, Robert Dong, May De León, Luis F. Álvarez, Eric Loaiza, Jose R. PLoS Negl Trop Dis Research Article Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens. Public Library of Science 2020-10-27 /pmc/articles/PMC7647123/ /pubmed/33108372 http://dx.doi.org/10.1371/journal.pntd.0008849 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Gittens, Rolando A.
Almanza, Alejandro
Bennett, Kelly L.
Mejía, Luis C.
Sanchez-Galan, Javier E.
Merchan, Fernando
Kern, Jonathan
Miller, Matthew J.
Esser, Helen J.
Hwang, Robert
Dong, May
De León, Luis F.
Álvarez, Eric
Loaiza, Jose R.
Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title_full Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title_fullStr Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title_full_unstemmed Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title_short Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
title_sort proteomic fingerprinting of neotropical hard tick species (acari: ixodidae) using a self-curated mass spectra reference library
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647123/
https://www.ncbi.nlm.nih.gov/pubmed/33108372
http://dx.doi.org/10.1371/journal.pntd.0008849
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