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Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod sp...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647123/ https://www.ncbi.nlm.nih.gov/pubmed/33108372 http://dx.doi.org/10.1371/journal.pntd.0008849 |
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author | Gittens, Rolando A. Almanza, Alejandro Bennett, Kelly L. Mejía, Luis C. Sanchez-Galan, Javier E. Merchan, Fernando Kern, Jonathan Miller, Matthew J. Esser, Helen J. Hwang, Robert Dong, May De León, Luis F. Álvarez, Eric Loaiza, Jose R. |
author_facet | Gittens, Rolando A. Almanza, Alejandro Bennett, Kelly L. Mejía, Luis C. Sanchez-Galan, Javier E. Merchan, Fernando Kern, Jonathan Miller, Matthew J. Esser, Helen J. Hwang, Robert Dong, May De León, Luis F. Álvarez, Eric Loaiza, Jose R. |
author_sort | Gittens, Rolando A. |
collection | PubMed |
description | Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens. |
format | Online Article Text |
id | pubmed-7647123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-76471232020-11-16 Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library Gittens, Rolando A. Almanza, Alejandro Bennett, Kelly L. Mejía, Luis C. Sanchez-Galan, Javier E. Merchan, Fernando Kern, Jonathan Miller, Matthew J. Esser, Helen J. Hwang, Robert Dong, May De León, Luis F. Álvarez, Eric Loaiza, Jose R. PLoS Negl Trop Dis Research Article Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry is an analytical method that detects macromolecules that can be used for proteomic fingerprinting and taxonomic identification in arthropods. The conventional MALDI approach uses fresh laboratory-reared arthropod specimens to build a reference mass spectra library with high-quality standards required to achieve reliable identification. However, this may not be possible to accomplish in some arthropod groups that are difficult to rear under laboratory conditions, or for which only alcohol preserved samples are available. Here, we generated MALDI mass spectra of highly abundant proteins from the legs of 18 Neotropical species of adult field-collected hard ticks, several of which had not been analyzed by mass spectrometry before. We then used their mass spectra as fingerprints to identify each tick species by applying machine learning and pattern recognition algorithms that combined unsupervised and supervised clustering approaches. Both Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) classification algorithms were able to identify spectra from different tick species, with LDA achieving the best performance when applied to field-collected specimens that did have an existing entry in a reference library of arthropod protein spectra. These findings contribute to the growing literature that ascertains mass spectrometry as a rapid and effective method to complement other well-established techniques for taxonomic identification of disease vectors, which is the first step to predict and manage arthropod-borne pathogens. Public Library of Science 2020-10-27 /pmc/articles/PMC7647123/ /pubmed/33108372 http://dx.doi.org/10.1371/journal.pntd.0008849 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Gittens, Rolando A. Almanza, Alejandro Bennett, Kelly L. Mejía, Luis C. Sanchez-Galan, Javier E. Merchan, Fernando Kern, Jonathan Miller, Matthew J. Esser, Helen J. Hwang, Robert Dong, May De León, Luis F. Álvarez, Eric Loaiza, Jose R. Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title | Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title_full | Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title_fullStr | Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title_full_unstemmed | Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title_short | Proteomic fingerprinting of Neotropical hard tick species (Acari: Ixodidae) using a self-curated mass spectra reference library |
title_sort | proteomic fingerprinting of neotropical hard tick species (acari: ixodidae) using a self-curated mass spectra reference library |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647123/ https://www.ncbi.nlm.nih.gov/pubmed/33108372 http://dx.doi.org/10.1371/journal.pntd.0008849 |
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