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Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms

Linezolid is an oxazolidinone class antibiotic used for treatment infections caused by various multidrug-resistant gram-positive pathogens including enterococci. However, recently, linezolid-resistant isolates in animals are considered as a human health hazard. In a broiler operation system, antimic...

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Autores principales: Yoon, Sunghyun, Kim, Yeong Bin, Seo, Kwang Won, Ha, Jong Su, Noh, Eun Bi, Lee, Young Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647823/
https://www.ncbi.nlm.nih.gov/pubmed/33142524
http://dx.doi.org/10.1016/j.psj.2020.06.087
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author Yoon, Sunghyun
Kim, Yeong Bin
Seo, Kwang Won
Ha, Jong Su
Noh, Eun Bi
Lee, Young Ju
author_facet Yoon, Sunghyun
Kim, Yeong Bin
Seo, Kwang Won
Ha, Jong Su
Noh, Eun Bi
Lee, Young Ju
author_sort Yoon, Sunghyun
collection PubMed
description Linezolid is an oxazolidinone class antibiotic used for treatment infections caused by various multidrug-resistant gram-positive pathogens including enterococci. However, recently, linezolid-resistant isolates in animals are considered as a human health hazard. In a broiler operation system, antimicrobial resistance can be transferred to the environment and commercial broiler via the fecal–oral route. Therefore, this study was conducted to investigate the prevalence and characteristics of linezolid-resistant Enterococcus faecalis (E. faecalis) from broiler parent stock in a broiler operation system. Among 297 E. faecalis isolates from 85 flocks in 8 broiler breeder farms, the prevalence of chloramphenicol- and linezolid-resistant isolates was 0 to 12.1% and 0 to 8.0%, respectively; however, there were no significant differences between farms. Therefore, a total of 14 (4.7%) chloramphenicol- and/or linezolid-resistant E. faecalis showed resistance to 7 or more antimicrobial classes. The drug-resistance gene optrA, which can confer resistance to linezolid, tedizolid, and phenicols, was found in 8 (2.69%) isolates, and 7 (2.36%) of the 8 optrA-positive isolates co-carried the phenicol exporter gene fexA. However, E. faecalis isolates from 3 of 8 broiler breeder farms only carried the optrA and/or fexA genes. As linezolid is one of the last antimicrobial treatments of choice for multidrug-resistant gram-positive pathogens including E. faecalis, the presence of antibiotic-resistant E. faecalis in broiler breeder farms should be monitored to prevent the introduction of linezolid-resistant strains to the food chain.
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spelling pubmed-76478232020-11-13 Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms Yoon, Sunghyun Kim, Yeong Bin Seo, Kwang Won Ha, Jong Su Noh, Eun Bi Lee, Young Ju Poult Sci Microbiology and Food Safety Linezolid is an oxazolidinone class antibiotic used for treatment infections caused by various multidrug-resistant gram-positive pathogens including enterococci. However, recently, linezolid-resistant isolates in animals are considered as a human health hazard. In a broiler operation system, antimicrobial resistance can be transferred to the environment and commercial broiler via the fecal–oral route. Therefore, this study was conducted to investigate the prevalence and characteristics of linezolid-resistant Enterococcus faecalis (E. faecalis) from broiler parent stock in a broiler operation system. Among 297 E. faecalis isolates from 85 flocks in 8 broiler breeder farms, the prevalence of chloramphenicol- and linezolid-resistant isolates was 0 to 12.1% and 0 to 8.0%, respectively; however, there were no significant differences between farms. Therefore, a total of 14 (4.7%) chloramphenicol- and/or linezolid-resistant E. faecalis showed resistance to 7 or more antimicrobial classes. The drug-resistance gene optrA, which can confer resistance to linezolid, tedizolid, and phenicols, was found in 8 (2.69%) isolates, and 7 (2.36%) of the 8 optrA-positive isolates co-carried the phenicol exporter gene fexA. However, E. faecalis isolates from 3 of 8 broiler breeder farms only carried the optrA and/or fexA genes. As linezolid is one of the last antimicrobial treatments of choice for multidrug-resistant gram-positive pathogens including E. faecalis, the presence of antibiotic-resistant E. faecalis in broiler breeder farms should be monitored to prevent the introduction of linezolid-resistant strains to the food chain. Elsevier 2020-08-15 /pmc/articles/PMC7647823/ /pubmed/33142524 http://dx.doi.org/10.1016/j.psj.2020.06.087 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology and Food Safety
Yoon, Sunghyun
Kim, Yeong Bin
Seo, Kwang Won
Ha, Jong Su
Noh, Eun Bi
Lee, Young Ju
Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title_full Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title_fullStr Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title_full_unstemmed Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title_short Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms
title_sort characteristics of linezolid-resistant enterococcus faecalis isolates from broiler breeder farms
topic Microbiology and Food Safety
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647823/
https://www.ncbi.nlm.nih.gov/pubmed/33142524
http://dx.doi.org/10.1016/j.psj.2020.06.087
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