Cargando…

1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese

This study was aimed to investigate whether 1-deoxynojirimycin (DNJ) affects the digestion system of young geese and assess whether mulberry leaf, which contains this substance, has disadvantages that compromise its value as poultry feed. One hundred and twenty-eight 12-day-old male Wanxi white gees...

Descripción completa

Detalles Bibliográficos
Autores principales: Hou, Qirui, Qian, Zhongyao, Wu, Ping, Shen, Manman, Li, Long, Zhao, Weiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647860/
https://www.ncbi.nlm.nih.gov/pubmed/33142503
http://dx.doi.org/10.1016/j.psj.2020.07.048
_version_ 1783606997631893504
author Hou, Qirui
Qian, Zhongyao
Wu, Ping
Shen, Manman
Li, Long
Zhao, Weiguo
author_facet Hou, Qirui
Qian, Zhongyao
Wu, Ping
Shen, Manman
Li, Long
Zhao, Weiguo
author_sort Hou, Qirui
collection PubMed
description This study was aimed to investigate whether 1-deoxynojirimycin (DNJ) affects the digestion system of young geese and assess whether mulberry leaf, which contains this substance, has disadvantages that compromise its value as poultry feed. One hundred and twenty-eight 12-day-old male Wanxi white geese were randomly assigned into 4 treatment groups. The control group was fed an ordinary diet without DNJ. The other groups namely L-DNJ, M-DNJ, and H-DNJ had their basic diets supplemented with 0.05 mg/g, 0.1 mg/g, and 0.15 mg/g DNJ, respectively. The geese were fed for 6 wk, and the apparent digestibility test was conducted in the last week. Intestinal parameters, digestive organs, and enzymes were determined. 16S rRNA gene sequencing was conducted for cecal flora composition. The results revealed that DNJ decreased body and liver weight and increased feed conversion ratio in comparison with the control (P < 0.05); however, it did not influence the weight and length of the intestine or the pancreas weight. The utilization of organic matter, metabolizable energy, ether extract, acid detergent fiber, and calcium in feed were reduced in the M-DNJ and L-DNJ groups compared with those in the control (P < 0.05); however, the utilization of crude protein was increased in all DNJ-treated groups (P < 0.01). In the H-DNJ group, the usage of soluble phosphorus was also increased (P < 0.05). High-dose DNJ increased the activity of trypsin in the pancreas but reduced those of amylase (P < 0.05) and lipase (P > 0.05) in the pancreas and duodenum. The intestinal villi were short, even impaired, in DNJ-treated groups. High-throughput sequencing data revealed that DNJ supplement reduced the α-diversity indices of the cecal microbiota. The principal component analysis further suggested a difference in community structure between the DNJ treatment groups and control. High-dose DNJ increased the relative abundance of Bacteroides, Escherichia-Shigella, and Butyricicoccus but reduced that of unclassified Ruminococcaceae compared with the control (P < 0.05). In conclusion, changes in the digestive system caused by DNJ seriously affected the metabolism of nutrients in geese and reduced their growth performance. Attention should be paid to the adverse effects of DNJ when using mulberry leaves as poultry feed.
format Online
Article
Text
id pubmed-7647860
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-76478602020-11-16 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese Hou, Qirui Qian, Zhongyao Wu, Ping Shen, Manman Li, Long Zhao, Weiguo Poult Sci Metabolism and Nutrition This study was aimed to investigate whether 1-deoxynojirimycin (DNJ) affects the digestion system of young geese and assess whether mulberry leaf, which contains this substance, has disadvantages that compromise its value as poultry feed. One hundred and twenty-eight 12-day-old male Wanxi white geese were randomly assigned into 4 treatment groups. The control group was fed an ordinary diet without DNJ. The other groups namely L-DNJ, M-DNJ, and H-DNJ had their basic diets supplemented with 0.05 mg/g, 0.1 mg/g, and 0.15 mg/g DNJ, respectively. The geese were fed for 6 wk, and the apparent digestibility test was conducted in the last week. Intestinal parameters, digestive organs, and enzymes were determined. 16S rRNA gene sequencing was conducted for cecal flora composition. The results revealed that DNJ decreased body and liver weight and increased feed conversion ratio in comparison with the control (P < 0.05); however, it did not influence the weight and length of the intestine or the pancreas weight. The utilization of organic matter, metabolizable energy, ether extract, acid detergent fiber, and calcium in feed were reduced in the M-DNJ and L-DNJ groups compared with those in the control (P < 0.05); however, the utilization of crude protein was increased in all DNJ-treated groups (P < 0.01). In the H-DNJ group, the usage of soluble phosphorus was also increased (P < 0.05). High-dose DNJ increased the activity of trypsin in the pancreas but reduced those of amylase (P < 0.05) and lipase (P > 0.05) in the pancreas and duodenum. The intestinal villi were short, even impaired, in DNJ-treated groups. High-throughput sequencing data revealed that DNJ supplement reduced the α-diversity indices of the cecal microbiota. The principal component analysis further suggested a difference in community structure between the DNJ treatment groups and control. High-dose DNJ increased the relative abundance of Bacteroides, Escherichia-Shigella, and Butyricicoccus but reduced that of unclassified Ruminococcaceae compared with the control (P < 0.05). In conclusion, changes in the digestive system caused by DNJ seriously affected the metabolism of nutrients in geese and reduced their growth performance. Attention should be paid to the adverse effects of DNJ when using mulberry leaves as poultry feed. Elsevier 2020-08-29 /pmc/articles/PMC7647860/ /pubmed/33142503 http://dx.doi.org/10.1016/j.psj.2020.07.048 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Metabolism and Nutrition
Hou, Qirui
Qian, Zhongyao
Wu, Ping
Shen, Manman
Li, Long
Zhao, Weiguo
1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title_full 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title_fullStr 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title_full_unstemmed 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title_short 1-Deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
title_sort 1-deoxynojirimycin from mulberry leaves changes gut digestion and microbiota composition in geese
topic Metabolism and Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647860/
https://www.ncbi.nlm.nih.gov/pubmed/33142503
http://dx.doi.org/10.1016/j.psj.2020.07.048
work_keys_str_mv AT houqirui 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese
AT qianzhongyao 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese
AT wuping 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese
AT shenmanman 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese
AT lilong 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese
AT zhaoweiguo 1deoxynojirimycinfrommulberryleaveschangesgutdigestionandmicrobiotacompositioningeese