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Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride

Mercuric chloride (HgCl(2)) is a widely distributed environmental pollutant with multiorgan toxicity including immune organs such as spleen. Selenium (Se) is an essential trace element in animal nutrition and exerts biological activity to antagonize organ toxicity caused by heavy metals. The objecti...

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Autores principales: Fan, Rui-Feng, Liu, Jiang-Xiu, Yan, Yu-Xue, Wang, Lin, Wang, Zhen-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647867/
https://www.ncbi.nlm.nih.gov/pubmed/33142460
http://dx.doi.org/10.1016/j.psj.2020.08.031
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author Fan, Rui-Feng
Liu, Jiang-Xiu
Yan, Yu-Xue
Wang, Lin
Wang, Zhen-Yong
author_facet Fan, Rui-Feng
Liu, Jiang-Xiu
Yan, Yu-Xue
Wang, Lin
Wang, Zhen-Yong
author_sort Fan, Rui-Feng
collection PubMed
description Mercuric chloride (HgCl(2)) is a widely distributed environmental pollutant with multiorgan toxicity including immune organs such as spleen. Selenium (Se) is an essential trace element in animal nutrition and exerts biological activity to antagonize organ toxicity caused by heavy metals. The objective of this study was to explore the underlying mechanism of the protective effects of Se against spleen damage caused by HgCl(2) in chicken. Ninety male Hyline brown chicken were randomly divided into 3 groups namely Cont, HgCl(2), and HgCl(2)+Se group. Chicken were provided with the standard diet and nontreated water, standard diet and HgCl(2)-treated water (250 ppm), and sodium selenite-treated diet (10 ppm) plus HgCl(2)-treated water (250 ppm), respectively. After being fed for 7 wk, the spleen tissues were collected, and spleen index, the microstructure of the spleen, and the indicators of oxidative stress, inflammation, apoptosis as well as heat shock proteins (HSP) were detected. First, the results of spleen index and pathological examination confirmed that Se exerted an antagonistic effect on the spleen injury induced by HgCl(2). Second, Se ameliorated HgCl(2)-induced oxidative stress by decreasing the level of malondialdehyde and increasing the levels of glutathione, glutathione peroxidase, and total antioxidant capacity. Third, Se attenuated HgCl(2)-induced inflammation by decreasing the protein expression of nuclear factor kappa-B, inducible nitric oxide synthase, and cyclooxygenase-2, and the gene expression of interleukin (IL)-1β, IL-6, IL-8, IL-12β, IL-18 as well as tumor necrosis factor-α. Fourth, Se inhibited HgCl(2)-induced apoptosis by downregulating the protein expression of BCL2 antagonist/killer 1 and upregulating the protein expression of B-cell lymphoma-2. Finally, Se reversed HgCl(2)-triggered activation of HSP 60, 70, and 90. In conclusion, Se antagonized HgCl(2)-induced spleen damage in chicken, partially through the regulation of oxidative stress, inflammatory, and apoptotic signaling.
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spelling pubmed-76478672020-11-16 Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride Fan, Rui-Feng Liu, Jiang-Xiu Yan, Yu-Xue Wang, Lin Wang, Zhen-Yong Poult Sci Immunology, Health and Disease Mercuric chloride (HgCl(2)) is a widely distributed environmental pollutant with multiorgan toxicity including immune organs such as spleen. Selenium (Se) is an essential trace element in animal nutrition and exerts biological activity to antagonize organ toxicity caused by heavy metals. The objective of this study was to explore the underlying mechanism of the protective effects of Se against spleen damage caused by HgCl(2) in chicken. Ninety male Hyline brown chicken were randomly divided into 3 groups namely Cont, HgCl(2), and HgCl(2)+Se group. Chicken were provided with the standard diet and nontreated water, standard diet and HgCl(2)-treated water (250 ppm), and sodium selenite-treated diet (10 ppm) plus HgCl(2)-treated water (250 ppm), respectively. After being fed for 7 wk, the spleen tissues were collected, and spleen index, the microstructure of the spleen, and the indicators of oxidative stress, inflammation, apoptosis as well as heat shock proteins (HSP) were detected. First, the results of spleen index and pathological examination confirmed that Se exerted an antagonistic effect on the spleen injury induced by HgCl(2). Second, Se ameliorated HgCl(2)-induced oxidative stress by decreasing the level of malondialdehyde and increasing the levels of glutathione, glutathione peroxidase, and total antioxidant capacity. Third, Se attenuated HgCl(2)-induced inflammation by decreasing the protein expression of nuclear factor kappa-B, inducible nitric oxide synthase, and cyclooxygenase-2, and the gene expression of interleukin (IL)-1β, IL-6, IL-8, IL-12β, IL-18 as well as tumor necrosis factor-α. Fourth, Se inhibited HgCl(2)-induced apoptosis by downregulating the protein expression of BCL2 antagonist/killer 1 and upregulating the protein expression of B-cell lymphoma-2. Finally, Se reversed HgCl(2)-triggered activation of HSP 60, 70, and 90. In conclusion, Se antagonized HgCl(2)-induced spleen damage in chicken, partially through the regulation of oxidative stress, inflammatory, and apoptotic signaling. Elsevier 2020-08-31 /pmc/articles/PMC7647867/ /pubmed/33142460 http://dx.doi.org/10.1016/j.psj.2020.08.031 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology, Health and Disease
Fan, Rui-Feng
Liu, Jiang-Xiu
Yan, Yu-Xue
Wang, Lin
Wang, Zhen-Yong
Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title_full Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title_fullStr Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title_full_unstemmed Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title_short Selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
title_sort selenium relieves oxidative stress, inflammation, and apoptosis within spleen of chicken exposed to mercuric chloride
topic Immunology, Health and Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647867/
https://www.ncbi.nlm.nih.gov/pubmed/33142460
http://dx.doi.org/10.1016/j.psj.2020.08.031
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