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A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration

Immune cell infiltration is an important indicator of whether tumor patients will benefit from immunotherapy. Gastric cancer is one of the most common tumors in the world, and new indicators of immunotherapy are urgently needed. The aim of this study was to construct ceRNA networks in gastric cancer...

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Autores principales: Zhang, Kai, Zhang, Lei, Mi, Yang, Tang, YouCai, Ren, FeiFei, Liu, Bin, Zhang, Yi, Zheng, PengYuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648034/
https://www.ncbi.nlm.nih.gov/pubmed/32860283
http://dx.doi.org/10.1111/cas.14634
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author Zhang, Kai
Zhang, Lei
Mi, Yang
Tang, YouCai
Ren, FeiFei
Liu, Bin
Zhang, Yi
Zheng, PengYuan
author_facet Zhang, Kai
Zhang, Lei
Mi, Yang
Tang, YouCai
Ren, FeiFei
Liu, Bin
Zhang, Yi
Zheng, PengYuan
author_sort Zhang, Kai
collection PubMed
description Immune cell infiltration is an important indicator of whether tumor patients will benefit from immunotherapy. Gastric cancer is one of the most common tumors in the world, and new indicators of immunotherapy are urgently needed. The aim of this study was to construct ceRNA networks in gastric cancer with different degrees of immune cell infiltration. We analyzed the expression profiles of different gastric cancer with different degrees of immune cell infiltration retrieved from The Cancer Genome Atlas (TCGA) database and found differentially expressed lncRNAs, mRNAs, and miRNAs. A ceRNA regulatory network of gastric cancer with different degrees of immune cell infiltration was constructed using functional annotation, RNA‐RNA interaction prediction, correlation analysis, survival analysis, and other comprehensive bioinformatics methods. The interaction and correlation between ceRNAs were verified using experiments on tumor tissues and cell lines. Cell line experiments showed a potential RP11‐1094M14.8/miR‐1269a/CXCL9 axis that was consistent with the ceRNA theory. qRT‐PCR results showed that RP11‐1094M14.8 knockdown significantly reduced the expression of CXCL9, and RP11‐1094M14.8 overexpression had the opposite effect. The results of clinical analysis of gastric cancer samples showed that RP11‐1094M14.8 and CXCL9 were highly expressed in hot tumors, and CXCL9 was positively correlated with a better prognosis for patients. The constructed novel ceRNA network and the potential regulatory axis may provide a comprehensive understanding of the potential mechanisms of development in gastric cancer with different degrees of immune cell infiltration. The RP11‐1094M14.8/miR‐1269a/CXCL9 axis may serve as a potential immune‐therapeutic target for gastric cancer with different degrees of immune cell infiltration.
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spelling pubmed-76480342020-11-16 A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration Zhang, Kai Zhang, Lei Mi, Yang Tang, YouCai Ren, FeiFei Liu, Bin Zhang, Yi Zheng, PengYuan Cancer Sci Original Articles Immune cell infiltration is an important indicator of whether tumor patients will benefit from immunotherapy. Gastric cancer is one of the most common tumors in the world, and new indicators of immunotherapy are urgently needed. The aim of this study was to construct ceRNA networks in gastric cancer with different degrees of immune cell infiltration. We analyzed the expression profiles of different gastric cancer with different degrees of immune cell infiltration retrieved from The Cancer Genome Atlas (TCGA) database and found differentially expressed lncRNAs, mRNAs, and miRNAs. A ceRNA regulatory network of gastric cancer with different degrees of immune cell infiltration was constructed using functional annotation, RNA‐RNA interaction prediction, correlation analysis, survival analysis, and other comprehensive bioinformatics methods. The interaction and correlation between ceRNAs were verified using experiments on tumor tissues and cell lines. Cell line experiments showed a potential RP11‐1094M14.8/miR‐1269a/CXCL9 axis that was consistent with the ceRNA theory. qRT‐PCR results showed that RP11‐1094M14.8 knockdown significantly reduced the expression of CXCL9, and RP11‐1094M14.8 overexpression had the opposite effect. The results of clinical analysis of gastric cancer samples showed that RP11‐1094M14.8 and CXCL9 were highly expressed in hot tumors, and CXCL9 was positively correlated with a better prognosis for patients. The constructed novel ceRNA network and the potential regulatory axis may provide a comprehensive understanding of the potential mechanisms of development in gastric cancer with different degrees of immune cell infiltration. The RP11‐1094M14.8/miR‐1269a/CXCL9 axis may serve as a potential immune‐therapeutic target for gastric cancer with different degrees of immune cell infiltration. John Wiley and Sons Inc. 2020-09-11 2020-11 /pmc/articles/PMC7648034/ /pubmed/32860283 http://dx.doi.org/10.1111/cas.14634 Text en © 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Zhang, Kai
Zhang, Lei
Mi, Yang
Tang, YouCai
Ren, FeiFei
Liu, Bin
Zhang, Yi
Zheng, PengYuan
A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title_full A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title_fullStr A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title_full_unstemmed A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title_short A ceRNA network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
title_sort cerna network and a potential regulatory axis in gastric cancer with different degrees of immune cell infiltration
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648034/
https://www.ncbi.nlm.nih.gov/pubmed/32860283
http://dx.doi.org/10.1111/cas.14634
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