Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation
BACKGROUND AND AIMS: Intestinal microbiota dysbiosis is implicated in Crohn’s disease [CD] and may play an important role in triggering postoperative disease recurrence [POR]. We prospectively studied faecal and mucosal microbial recolonisation following ileocaecal resection to identify the predicti...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648170/ https://www.ncbi.nlm.nih.gov/pubmed/32333762 http://dx.doi.org/10.1093/ecco-jcc/jjaa081 |
_version_ | 1783607059321716736 |
---|---|
author | Machiels, Kathleen Pozuelo del Río, Marta Martinez-De la Torre, Adrian Xie, Zixuan Pascal Andreu, Victòria Sabino, João Santiago, Alba Campos, David Wolthuis, Albert D’Hoore, André De Hertogh, Gert Ferrante, Marc Manichanh, Chaysavanh Vermeire, Séverine |
author_facet | Machiels, Kathleen Pozuelo del Río, Marta Martinez-De la Torre, Adrian Xie, Zixuan Pascal Andreu, Victòria Sabino, João Santiago, Alba Campos, David Wolthuis, Albert D’Hoore, André De Hertogh, Gert Ferrante, Marc Manichanh, Chaysavanh Vermeire, Séverine |
author_sort | Machiels, Kathleen |
collection | PubMed |
description | BACKGROUND AND AIMS: Intestinal microbiota dysbiosis is implicated in Crohn’s disease [CD] and may play an important role in triggering postoperative disease recurrence [POR]. We prospectively studied faecal and mucosal microbial recolonisation following ileocaecal resection to identify the predictive value of recurrence-related microbiota. METHODS: Mucosal and/or faecal samples from 121 CD patients undergoing ileocaecal resection were collected at predefined time points before and after surgery. Ileal biopsies were collected from 39 healthy controls. POR was defined by a Rutgeerts score ≥i2b. The microbiota was evaluated by 16S rRNA sequencing. Prediction analysis was performed using C5.0 and Random Forest algorithms. RESULTS: The mucosa-associated microbiota in CD patients was characterised by a depletion of butyrate-producing species (false discovery rate [FDR] <0.01) and enrichment of Proteobacteria [FDR = 0.009] and Akkermansia spp. [FDR = 0.02]. Following resection, a mucosal enrichment of Lachnospiraceae [FDR <0.001] was seen in all patients but in POR patients, also Fusobacteriaceae [FDR <0.001] increased compared with baseline. Patients without POR showed a decrease of Streptococcaceae [FDR = 0.003] and Actinomycineae [FDR = 0.06]. The mucosa-associated microbiota profile had good discriminative power to predict POR, and was superior to clinical risk factors. At Month 6, patients experiencing POR had a higher abundance of taxa belonging to Negativicutes [FDR = 0.04] and Fusobacteria [FDR = 0.04] compared with patients without POR. CONCLUSIONS: Microbiota recolonisation after ileocaecal resection is different between recurrence and non-recurrence patients, with Fusobacteria as the most prominent player driving early POR. These bacteria involved in the early recolonisation and POR represent a promising therapeutic strategy in the prevention of disease recurrence. |
format | Online Article Text |
id | pubmed-7648170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-76481702020-11-12 Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation Machiels, Kathleen Pozuelo del Río, Marta Martinez-De la Torre, Adrian Xie, Zixuan Pascal Andreu, Victòria Sabino, João Santiago, Alba Campos, David Wolthuis, Albert D’Hoore, André De Hertogh, Gert Ferrante, Marc Manichanh, Chaysavanh Vermeire, Séverine J Crohns Colitis Original Articles BACKGROUND AND AIMS: Intestinal microbiota dysbiosis is implicated in Crohn’s disease [CD] and may play an important role in triggering postoperative disease recurrence [POR]. We prospectively studied faecal and mucosal microbial recolonisation following ileocaecal resection to identify the predictive value of recurrence-related microbiota. METHODS: Mucosal and/or faecal samples from 121 CD patients undergoing ileocaecal resection were collected at predefined time points before and after surgery. Ileal biopsies were collected from 39 healthy controls. POR was defined by a Rutgeerts score ≥i2b. The microbiota was evaluated by 16S rRNA sequencing. Prediction analysis was performed using C5.0 and Random Forest algorithms. RESULTS: The mucosa-associated microbiota in CD patients was characterised by a depletion of butyrate-producing species (false discovery rate [FDR] <0.01) and enrichment of Proteobacteria [FDR = 0.009] and Akkermansia spp. [FDR = 0.02]. Following resection, a mucosal enrichment of Lachnospiraceae [FDR <0.001] was seen in all patients but in POR patients, also Fusobacteriaceae [FDR <0.001] increased compared with baseline. Patients without POR showed a decrease of Streptococcaceae [FDR = 0.003] and Actinomycineae [FDR = 0.06]. The mucosa-associated microbiota profile had good discriminative power to predict POR, and was superior to clinical risk factors. At Month 6, patients experiencing POR had a higher abundance of taxa belonging to Negativicutes [FDR = 0.04] and Fusobacteria [FDR = 0.04] compared with patients without POR. CONCLUSIONS: Microbiota recolonisation after ileocaecal resection is different between recurrence and non-recurrence patients, with Fusobacteria as the most prominent player driving early POR. These bacteria involved in the early recolonisation and POR represent a promising therapeutic strategy in the prevention of disease recurrence. Oxford University Press 2020-04-25 /pmc/articles/PMC7648170/ /pubmed/32333762 http://dx.doi.org/10.1093/ecco-jcc/jjaa081 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of European Crohn’s and Colitis Organisation. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Original Articles Machiels, Kathleen Pozuelo del Río, Marta Martinez-De la Torre, Adrian Xie, Zixuan Pascal Andreu, Victòria Sabino, João Santiago, Alba Campos, David Wolthuis, Albert D’Hoore, André De Hertogh, Gert Ferrante, Marc Manichanh, Chaysavanh Vermeire, Séverine Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title | Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title_full | Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title_fullStr | Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title_full_unstemmed | Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title_short | Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation |
title_sort | early postoperative endoscopic recurrence in crohn’s disease is characterised by distinct microbiota recolonisation |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648170/ https://www.ncbi.nlm.nih.gov/pubmed/32333762 http://dx.doi.org/10.1093/ecco-jcc/jjaa081 |
work_keys_str_mv | AT machielskathleen earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT pozuelodelriomarta earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT martinezdelatorreadrian earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT xiezixuan earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT pascalandreuvictoria earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT sabinojoao earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT santiagoalba earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT camposdavid earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT wolthuisalbert earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT dhooreandre earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT dehertoghgert earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT ferrantemarc earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT manichanhchaysavanh earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation AT vermeireseverine earlypostoperativeendoscopicrecurrenceincrohnsdiseaseischaracterisedbydistinctmicrobiotarecolonisation |