Cargando…

Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies

OBJECTIVES: The aim of this study is to establish a sterilization method for cellulose nanofibers (CNFs) dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs, and to provide useful information for sample preparati...

Descripción completa

Detalles Bibliográficos
Autores principales: Sai, Takafumi, Maru, Junko, Obara, Sawae, Endoh, Shigehisa, Kajihara, Hideo, Fujita, Katsuhide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648261/
https://www.ncbi.nlm.nih.gov/pubmed/33159502
http://dx.doi.org/10.1002/1348-9585.12176
_version_ 1783607079965032448
author Sai, Takafumi
Maru, Junko
Obara, Sawae
Endoh, Shigehisa
Kajihara, Hideo
Fujita, Katsuhide
author_facet Sai, Takafumi
Maru, Junko
Obara, Sawae
Endoh, Shigehisa
Kajihara, Hideo
Fujita, Katsuhide
author_sort Sai, Takafumi
collection PubMed
description OBJECTIVES: The aim of this study is to establish a sterilization method for cellulose nanofibers (CNFs) dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs, and to provide useful information for sample preparation in future toxicity study of CNFs. METHODS: Various preservatives were added to the phosphorylated CNF dispersions, endotoxin level and the numbers of bacteria and fungi in the CNF dispersion were analyzed. The pH values and viscosity of sterilized CNF dispersions were compared with those of control and autoclaved CNF dispersions. RESULTS: Phosphorylated CNF dispersions at a concentration of 2.0 mg/mL or lower and the addition of 10 µg/mL benzalkonium chloride alone or 250 µg/mL methyl parahydroxybenzoate and 250 µg/mL propyl parahydroxybenzoate in combination can sterilize CNF dispersions without changing the physical and chemical properties of CNFs. CONCLUSIONS: We developed sterilization method for CNF dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs. This sterilization method for CNFs dispersions can be applied to the safety assessment of CNF with different physicochemical properties in the future.
format Online
Article
Text
id pubmed-7648261
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-76482612020-11-16 Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies Sai, Takafumi Maru, Junko Obara, Sawae Endoh, Shigehisa Kajihara, Hideo Fujita, Katsuhide J Occup Health Original Articles OBJECTIVES: The aim of this study is to establish a sterilization method for cellulose nanofibers (CNFs) dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs, and to provide useful information for sample preparation in future toxicity study of CNFs. METHODS: Various preservatives were added to the phosphorylated CNF dispersions, endotoxin level and the numbers of bacteria and fungi in the CNF dispersion were analyzed. The pH values and viscosity of sterilized CNF dispersions were compared with those of control and autoclaved CNF dispersions. RESULTS: Phosphorylated CNF dispersions at a concentration of 2.0 mg/mL or lower and the addition of 10 µg/mL benzalkonium chloride alone or 250 µg/mL methyl parahydroxybenzoate and 250 µg/mL propyl parahydroxybenzoate in combination can sterilize CNF dispersions without changing the physical and chemical properties of CNFs. CONCLUSIONS: We developed sterilization method for CNF dispersions that uses multiple preservatives with different hydrophilicities without affecting the physical and chemical properties of CNFs. This sterilization method for CNFs dispersions can be applied to the safety assessment of CNF with different physicochemical properties in the future. John Wiley and Sons Inc. 2020-11-07 /pmc/articles/PMC7648261/ /pubmed/33159502 http://dx.doi.org/10.1002/1348-9585.12176 Text en © 2020 The Authors. Journal of Occupational Health published by John Wiley & Sons Australia, Ltd on behalf of The Japan Society for Occupational Health This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sai, Takafumi
Maru, Junko
Obara, Sawae
Endoh, Shigehisa
Kajihara, Hideo
Fujita, Katsuhide
Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title_full Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title_fullStr Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title_full_unstemmed Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title_short Screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
title_sort screening of preservatives and evaluation of sterilized cellulose nanofibers for toxicity studies
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648261/
https://www.ncbi.nlm.nih.gov/pubmed/33159502
http://dx.doi.org/10.1002/1348-9585.12176
work_keys_str_mv AT saitakafumi screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies
AT marujunko screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies
AT obarasawae screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies
AT endohshigehisa screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies
AT kajiharahideo screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies
AT fujitakatsuhide screeningofpreservativesandevaluationofsterilizedcellulosenanofibersfortoxicitystudies