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Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements

Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain lar...

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Autores principales: Pinilla-Redondo, Rafael, Shehreen, Saadlee, Marino, Nicole D., Fagerlund, Robert D., Brown, Chris M., Sørensen, Søren J., Fineran, Peter C., Bondy-Denomy, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648647/
https://www.ncbi.nlm.nih.gov/pubmed/33159058
http://dx.doi.org/10.1038/s41467-020-19415-3
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author Pinilla-Redondo, Rafael
Shehreen, Saadlee
Marino, Nicole D.
Fagerlund, Robert D.
Brown, Chris M.
Sørensen, Søren J.
Fineran, Peter C.
Bondy-Denomy, Joseph
author_facet Pinilla-Redondo, Rafael
Shehreen, Saadlee
Marino, Nicole D.
Fagerlund, Robert D.
Brown, Chris M.
Sørensen, Søren J.
Fineran, Peter C.
Bondy-Denomy, Joseph
author_sort Pinilla-Redondo, Rafael
collection PubMed
description Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems.
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spelling pubmed-76486472020-11-10 Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements Pinilla-Redondo, Rafael Shehreen, Saadlee Marino, Nicole D. Fagerlund, Robert D. Brown, Chris M. Sørensen, Søren J. Fineran, Peter C. Bondy-Denomy, Joseph Nat Commun Article Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic elements (MGEs). In response, some MGEs have developed strategies to bypass immunity, including anti-CRISPR (Acr) proteins; yet the diversity, distribution and spectrum of activity of this immune evasion strategy remain largely unknown. Here, we report the discovery of new Acrs by assaying candidate genes adjacent to a conserved Acr-associated (Aca) gene, aca5, against a panel of six type I systems: I–F (Pseudomonas, Pectobacterium, and Serratia), I–E (Pseudomonas and Serratia), and I–C (Pseudomonas). We uncover 11 type I–F and/or I–E anti-CRISPR genes encoded on chromosomal and extrachromosomal MGEs within Enterobacteriaceae and Pseudomonas, and an additional Aca (aca9). The acr genes not only associate with other acr genes, but also with genes encoding inhibitors of distinct bacterial defense systems. Thus, our findings highlight the potential exploitation of acr loci neighborhoods for the identification of previously undescribed anti-defense systems. Nature Publishing Group UK 2020-11-06 /pmc/articles/PMC7648647/ /pubmed/33159058 http://dx.doi.org/10.1038/s41467-020-19415-3 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pinilla-Redondo, Rafael
Shehreen, Saadlee
Marino, Nicole D.
Fagerlund, Robert D.
Brown, Chris M.
Sørensen, Søren J.
Fineran, Peter C.
Bondy-Denomy, Joseph
Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title_full Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title_fullStr Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title_full_unstemmed Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title_short Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
title_sort discovery of multiple anti-crisprs highlights anti-defense gene clustering in mobile genetic elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648647/
https://www.ncbi.nlm.nih.gov/pubmed/33159058
http://dx.doi.org/10.1038/s41467-020-19415-3
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