The smfBox is an open-source platform for single-molecule FRET

Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s...

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Detalles Bibliográficos
Autores principales: Ambrose, Benjamin, Baxter, James M., Cully, John, Willmott, Matthew, Steele, Elliot M., Bateman, Benji C., Martin-Fernandez, Marisa L., Cadby, Ashley, Shewring, Jonathan, Aaldering, Marleen, Craggs, Timothy D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648814/
https://www.ncbi.nlm.nih.gov/pubmed/33159061
http://dx.doi.org/10.1038/s41467-020-19468-4
Descripción
Sumario:Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.

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