The smfBox is an open-source platform for single-molecule FRET

Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s...

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Autores principales: Ambrose, Benjamin, Baxter, James M., Cully, John, Willmott, Matthew, Steele, Elliot M., Bateman, Benji C., Martin-Fernandez, Marisa L., Cadby, Ashley, Shewring, Jonathan, Aaldering, Marleen, Craggs, Timothy D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648814/
https://www.ncbi.nlm.nih.gov/pubmed/33159061
http://dx.doi.org/10.1038/s41467-020-19468-4
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author Ambrose, Benjamin
Baxter, James M.
Cully, John
Willmott, Matthew
Steele, Elliot M.
Bateman, Benji C.
Martin-Fernandez, Marisa L.
Cadby, Ashley
Shewring, Jonathan
Aaldering, Marleen
Craggs, Timothy D.
author_facet Ambrose, Benjamin
Baxter, James M.
Cully, John
Willmott, Matthew
Steele, Elliot M.
Bateman, Benji C.
Martin-Fernandez, Marisa L.
Cadby, Ashley
Shewring, Jonathan
Aaldering, Marleen
Craggs, Timothy D.
author_sort Ambrose, Benjamin
collection PubMed
description Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.
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spelling pubmed-76488142020-11-10 The smfBox is an open-source platform for single-molecule FRET Ambrose, Benjamin Baxter, James M. Cully, John Willmott, Matthew Steele, Elliot M. Bateman, Benji C. Martin-Fernandez, Marisa L. Cadby, Ashley Shewring, Jonathan Aaldering, Marleen Craggs, Timothy D. Nat Commun Article Single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRET’s broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community. Nature Publishing Group UK 2020-11-06 /pmc/articles/PMC7648814/ /pubmed/33159061 http://dx.doi.org/10.1038/s41467-020-19468-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ambrose, Benjamin
Baxter, James M.
Cully, John
Willmott, Matthew
Steele, Elliot M.
Bateman, Benji C.
Martin-Fernandez, Marisa L.
Cadby, Ashley
Shewring, Jonathan
Aaldering, Marleen
Craggs, Timothy D.
The smfBox is an open-source platform for single-molecule FRET
title The smfBox is an open-source platform for single-molecule FRET
title_full The smfBox is an open-source platform for single-molecule FRET
title_fullStr The smfBox is an open-source platform for single-molecule FRET
title_full_unstemmed The smfBox is an open-source platform for single-molecule FRET
title_short The smfBox is an open-source platform for single-molecule FRET
title_sort smfbox is an open-source platform for single-molecule fret
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7648814/
https://www.ncbi.nlm.nih.gov/pubmed/33159061
http://dx.doi.org/10.1038/s41467-020-19468-4
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