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Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers
OBJECTIVE: This study was conducted to investigate the effects of dietary corn resistant starch (RS) on the intestinal morphology and barrier functions of broilers. METHODS: A total of 320 one-day-old broilers were randomly allocated to 5 dietary treatments: one normal corn–soybean (NC) diet, one co...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649406/ https://www.ncbi.nlm.nih.gov/pubmed/32164060 http://dx.doi.org/10.5713/ajas.19.0967 |
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author | Zhang, Yingying Liu, Yingsen Li, Jiaolong Xing, Tong Jiang, Yun Zhang, Lin Gao, Feng |
author_facet | Zhang, Yingying Liu, Yingsen Li, Jiaolong Xing, Tong Jiang, Yun Zhang, Lin Gao, Feng |
author_sort | Zhang, Yingying |
collection | PubMed |
description | OBJECTIVE: This study was conducted to investigate the effects of dietary corn resistant starch (RS) on the intestinal morphology and barrier functions of broilers. METHODS: A total of 320 one-day-old broilers were randomly allocated to 5 dietary treatments: one normal corn–soybean (NC) diet, one corn–soybean–based diet supplementation with 20% corn starch (CS), and 3 corn–soybean–based diets supplementation with 4%, 8%, and 12% corn resistant starch (RS) (identified as 4% RS, 8% RS, and 12% RS, respectively). Each group had eight replicates with eight broilers per replicate. After 21 days feeding, one bird with a body weight (BW) close to the average BW of their replicate was selected and slaughtered. The samples of duodenum, jejunum, ileum, caecum digesta, and blood were collected. RESULTS: Birds fed 4% RS, 8% RS and 12% RS diets showed lower feed intake, BW gain, jejunal villus height (VH), duodenal crypt depth (CD), jejunal VH/CD ratio, duodenal goblet cell density as well as mucin1 mRNA expressions compared to the NC group, but showed higher concentrations of cecal acetic acid and butyric acid, percentage of jejunal proliferating cell nuclear antigen-positive cells and delta like canonical Notch ligand 4 (Dll4), and hes family bHLH transcription factor 1 mRNA expressions. However, there were no differences on the plasma diamine oxidase activity and D-lactic acid concentration among all groups. CONCLUSION: These findings suggested that RS could suppress intestinal morphology and barrier functions by activating Notch pathway and inhibiting the development of goblet cells, resulting in decreased mucins and tight junction mRNA expression. |
format | Online Article Text |
id | pubmed-7649406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-76494062020-12-01 Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers Zhang, Yingying Liu, Yingsen Li, Jiaolong Xing, Tong Jiang, Yun Zhang, Lin Gao, Feng Asian-Australas J Anim Sci Article OBJECTIVE: This study was conducted to investigate the effects of dietary corn resistant starch (RS) on the intestinal morphology and barrier functions of broilers. METHODS: A total of 320 one-day-old broilers were randomly allocated to 5 dietary treatments: one normal corn–soybean (NC) diet, one corn–soybean–based diet supplementation with 20% corn starch (CS), and 3 corn–soybean–based diets supplementation with 4%, 8%, and 12% corn resistant starch (RS) (identified as 4% RS, 8% RS, and 12% RS, respectively). Each group had eight replicates with eight broilers per replicate. After 21 days feeding, one bird with a body weight (BW) close to the average BW of their replicate was selected and slaughtered. The samples of duodenum, jejunum, ileum, caecum digesta, and blood were collected. RESULTS: Birds fed 4% RS, 8% RS and 12% RS diets showed lower feed intake, BW gain, jejunal villus height (VH), duodenal crypt depth (CD), jejunal VH/CD ratio, duodenal goblet cell density as well as mucin1 mRNA expressions compared to the NC group, but showed higher concentrations of cecal acetic acid and butyric acid, percentage of jejunal proliferating cell nuclear antigen-positive cells and delta like canonical Notch ligand 4 (Dll4), and hes family bHLH transcription factor 1 mRNA expressions. However, there were no differences on the plasma diamine oxidase activity and D-lactic acid concentration among all groups. CONCLUSION: These findings suggested that RS could suppress intestinal morphology and barrier functions by activating Notch pathway and inhibiting the development of goblet cells, resulting in decreased mucins and tight junction mRNA expression. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2020-12 2020-03-12 /pmc/articles/PMC7649406/ /pubmed/32164060 http://dx.doi.org/10.5713/ajas.19.0967 Text en Copyright © 2020 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Zhang, Yingying Liu, Yingsen Li, Jiaolong Xing, Tong Jiang, Yun Zhang, Lin Gao, Feng Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title | Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title_full | Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title_fullStr | Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title_full_unstemmed | Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title_short | Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers |
title_sort | dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the notch signaling pathway of broilers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7649406/ https://www.ncbi.nlm.nih.gov/pubmed/32164060 http://dx.doi.org/10.5713/ajas.19.0967 |
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