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Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)
OBJECTIVE: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient m...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650214/ https://www.ncbi.nlm.nih.gov/pubmed/32883361 http://dx.doi.org/10.1186/s13104-020-05257-1 |
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author | Restrepo-Osorio, Catalina Gil-Correal, Alejandro Chamorro-Gutiérrez, Lina Ramírez-Ríos, Viviana Álvarez, Javier C. Villanueva-Mejía, Diego |
author_facet | Restrepo-Osorio, Catalina Gil-Correal, Alejandro Chamorro-Gutiérrez, Lina Ramírez-Ríos, Viviana Álvarez, Javier C. Villanueva-Mejía, Diego |
author_sort | Restrepo-Osorio, Catalina |
collection | PubMed |
description | OBJECTIVE: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. RESULTS: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. |
format | Online Article Text |
id | pubmed-7650214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-76502142020-11-09 Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) Restrepo-Osorio, Catalina Gil-Correal, Alejandro Chamorro-Gutiérrez, Lina Ramírez-Ríos, Viviana Álvarez, Javier C. Villanueva-Mejía, Diego BMC Res Notes Research Note OBJECTIVE: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. RESULTS: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. BioMed Central 2020-09-03 /pmc/articles/PMC7650214/ /pubmed/32883361 http://dx.doi.org/10.1186/s13104-020-05257-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Note Restrepo-Osorio, Catalina Gil-Correal, Alejandro Chamorro-Gutiérrez, Lina Ramírez-Ríos, Viviana Álvarez, Javier C. Villanueva-Mejía, Diego Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title | Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title_full | Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title_fullStr | Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title_full_unstemmed | Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title_short | Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) |
title_sort | efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (plukenetia volubilis l.) |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650214/ https://www.ncbi.nlm.nih.gov/pubmed/32883361 http://dx.doi.org/10.1186/s13104-020-05257-1 |
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