Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells

The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropr...

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Autores principales: Kajikawa, Keita, Imaizumi, Kent, Shinozaki, Munehisa, Shibata, Shinsuke, Shindo, Tomoko, Kitagawa, Takahiro, Shibata, Reo, Kamata, Yasuhiro, Kojima, Kota, Nagoshi, Narihito, Matsumoto, Morio, Nakamura, Masaya, Okano, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650268/
https://www.ncbi.nlm.nih.gov/pubmed/32883317
http://dx.doi.org/10.1186/s13041-020-00662-w
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author Kajikawa, Keita
Imaizumi, Kent
Shinozaki, Munehisa
Shibata, Shinsuke
Shindo, Tomoko
Kitagawa, Takahiro
Shibata, Reo
Kamata, Yasuhiro
Kojima, Kota
Nagoshi, Narihito
Matsumoto, Morio
Nakamura, Masaya
Okano, Hideyuki
author_facet Kajikawa, Keita
Imaizumi, Kent
Shinozaki, Munehisa
Shibata, Shinsuke
Shindo, Tomoko
Kitagawa, Takahiro
Shibata, Reo
Kamata, Yasuhiro
Kojima, Kota
Nagoshi, Narihito
Matsumoto, Morio
Nakamura, Masaya
Okano, Hideyuki
author_sort Kajikawa, Keita
collection PubMed
description The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.
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spelling pubmed-76502682020-11-09 Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells Kajikawa, Keita Imaizumi, Kent Shinozaki, Munehisa Shibata, Shinsuke Shindo, Tomoko Kitagawa, Takahiro Shibata, Reo Kamata, Yasuhiro Kojima, Kota Nagoshi, Narihito Matsumoto, Morio Nakamura, Masaya Okano, Hideyuki Mol Brain Research The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI. BioMed Central 2020-09-03 /pmc/articles/PMC7650268/ /pubmed/32883317 http://dx.doi.org/10.1186/s13041-020-00662-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kajikawa, Keita
Imaizumi, Kent
Shinozaki, Munehisa
Shibata, Shinsuke
Shindo, Tomoko
Kitagawa, Takahiro
Shibata, Reo
Kamata, Yasuhiro
Kojima, Kota
Nagoshi, Narihito
Matsumoto, Morio
Nakamura, Masaya
Okano, Hideyuki
Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title_full Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title_fullStr Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title_full_unstemmed Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title_short Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells
title_sort cell therapy for spinal cord injury by using human ipsc-derived region-specific neural progenitor cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650268/
https://www.ncbi.nlm.nih.gov/pubmed/32883317
http://dx.doi.org/10.1186/s13041-020-00662-w
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