Cargando…
Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds
Seed aging has an important effect on the germplasm preservation and industrialized production of Siberian wildrye (Elymus sibiricus) in the Qinghai-Tibet Plateau. However, so far its underlying molecular mechanisms still largely remain unknown. To shed light on this topic, one-year stored seeds of...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650541/ https://www.ncbi.nlm.nih.gov/pubmed/33076425 http://dx.doi.org/10.3390/plants9101370 |
_version_ | 1783607499721539584 |
---|---|
author | Lei, Xiong Liu, Wenhui Zhao, Junming You, Minghong Xiong, Chaohui Xiong, Yi Xiong, Yanli Yu, Qingqing Bai, Shiqie Ma, Xiao |
author_facet | Lei, Xiong Liu, Wenhui Zhao, Junming You, Minghong Xiong, Chaohui Xiong, Yi Xiong, Yanli Yu, Qingqing Bai, Shiqie Ma, Xiao |
author_sort | Lei, Xiong |
collection | PubMed |
description | Seed aging has an important effect on the germplasm preservation and industrialized production of Siberian wildrye (Elymus sibiricus) in the Qinghai-Tibet Plateau. However, so far its underlying molecular mechanisms still largely remain unknown. To shed light on this topic, one-year stored seeds of E. sibiricus were exposed to artificial aging treatments (AAT), followed by seed vigor characteristics and physiological status monitoring. Then global proteomics analysis was undertaken by the tandem mass tags (TMT) technique, and the proteins were quantified with liquid chromatography-tandem mass spectrometry on three aging time points (0 h, 36 h and 72 h). Finally, we verified the expression of related proteins by parallel reaction monitoring (PRM). Our results demonstrated that the seed vigor decreased remarkably in response to artificial aging, but the relative ion-leakage and malondialdehyde content, superoxide anion and hydrogen peroxide showed the opposite situation. Proteomic results showed that a total of 4169 proteins were identified and quantified. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that a series of key pathways including carbohydrate metabolism, lipid metabolism, and antioxidant activity were severely damaged by aging treatments. Numerous key proteins such as glyceraldehyde triphosphate glyceraldehyde dehydrogenase, succinate dehydrogenase, lipoxygenase, peroxidase, glutathione-s-transferase and late embryogenesis abundant proteins were significantly down-regulated. However, the up-regulation of the heat shock protein family has made a positive contribution to oxidative stress resistance in seeds. This study provides a useful catalog of the E. sibiricus proteomes with insights into the future genetic improvement of seed storability. |
format | Online Article Text |
id | pubmed-7650541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76505412020-11-10 Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds Lei, Xiong Liu, Wenhui Zhao, Junming You, Minghong Xiong, Chaohui Xiong, Yi Xiong, Yanli Yu, Qingqing Bai, Shiqie Ma, Xiao Plants (Basel) Article Seed aging has an important effect on the germplasm preservation and industrialized production of Siberian wildrye (Elymus sibiricus) in the Qinghai-Tibet Plateau. However, so far its underlying molecular mechanisms still largely remain unknown. To shed light on this topic, one-year stored seeds of E. sibiricus were exposed to artificial aging treatments (AAT), followed by seed vigor characteristics and physiological status monitoring. Then global proteomics analysis was undertaken by the tandem mass tags (TMT) technique, and the proteins were quantified with liquid chromatography-tandem mass spectrometry on three aging time points (0 h, 36 h and 72 h). Finally, we verified the expression of related proteins by parallel reaction monitoring (PRM). Our results demonstrated that the seed vigor decreased remarkably in response to artificial aging, but the relative ion-leakage and malondialdehyde content, superoxide anion and hydrogen peroxide showed the opposite situation. Proteomic results showed that a total of 4169 proteins were identified and quantified. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that a series of key pathways including carbohydrate metabolism, lipid metabolism, and antioxidant activity were severely damaged by aging treatments. Numerous key proteins such as glyceraldehyde triphosphate glyceraldehyde dehydrogenase, succinate dehydrogenase, lipoxygenase, peroxidase, glutathione-s-transferase and late embryogenesis abundant proteins were significantly down-regulated. However, the up-regulation of the heat shock protein family has made a positive contribution to oxidative stress resistance in seeds. This study provides a useful catalog of the E. sibiricus proteomes with insights into the future genetic improvement of seed storability. MDPI 2020-10-15 /pmc/articles/PMC7650541/ /pubmed/33076425 http://dx.doi.org/10.3390/plants9101370 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lei, Xiong Liu, Wenhui Zhao, Junming You, Minghong Xiong, Chaohui Xiong, Yi Xiong, Yanli Yu, Qingqing Bai, Shiqie Ma, Xiao Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title | Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title_full | Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title_fullStr | Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title_full_unstemmed | Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title_short | Comparative Physiological and Proteomic Analysis Reveals Different Involvement of Proteins during Artificial Aging of Siberian Wildrye Seeds |
title_sort | comparative physiological and proteomic analysis reveals different involvement of proteins during artificial aging of siberian wildrye seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7650541/ https://www.ncbi.nlm.nih.gov/pubmed/33076425 http://dx.doi.org/10.3390/plants9101370 |
work_keys_str_mv | AT leixiong comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT liuwenhui comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT zhaojunming comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT youminghong comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT xiongchaohui comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT xiongyi comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT xiongyanli comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT yuqingqing comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT baishiqie comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds AT maxiao comparativephysiologicalandproteomicanalysisrevealsdifferentinvolvementofproteinsduringartificialagingofsiberianwildryeseeds |